ISSN:
1432-1424
Keywords:
cell potential
;
amiloride
;
sodium transport
;
reversal potential
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Summary Knowledge of the voltage dependencies of apical and basolateral conductances is important in determining the factors that regulate transcellular transport. To gain this knowledge it is necessary to distinguish between cellular and paracellular currents and conductances. This is generally done by sequentially measuring transepithelial current/voltage (I t /V t ) and conductance/voltage (g t /V t ) relationships before and after the abolition of cellular sodium transport with amiloride. Often, however, there are variable time-dependent and voltage-dependent responses to voltage perturbation both in the absence and presence of amiloride, pointing to effects on the paracellular pathway. We have here investigated these phenomena systematically and found that the difficulties were significantly lessened by the use of an intermittent technique, measuringI t andg t before and after brief (〈10 sec) exposure to amiloride at each setting ofV t .I/V relationships were characterized by these means in frog skins (Rana pipiens, Northern variety, andRana temporaria). Cellular current,I c , decreased with hyperpolarization (larger serosa positive clamps) ofV t . DerivedI c /V t relationships betweenV t =0 and 175 mV (serosa positive) were slightly concave upwards. Because values of cell conductance,g c , remained finite, it was possible to demonstrate reversal ofI c . Values of the reversal potentialV' averaged 156±14 (sd,n=18) mV. Simultaneous microelectrode measurements permitted also the calculation of apical and basolateral conductances,g a andg b . The apical conductance decreased monotonically with increasing positivity ofV t (andV a ). In contrast, in the range in which the basolateral conductance could be evaluated adequately (V t 〈125 mV),g b increased with more positive values ofV t (andV b ). That is, there was an inverse relation betweeng b and cellular current at the quasi-steady state, 10–30 sec after the transepithelial voltage step.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01871763
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