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  • Artikel: DFG Deutsche Nationallizenzen  (3)
  • 1985-1989  (3)
  • CLIMSIM  (1)
  • Immunoglobulins  (1)
  • MAT 13762 cells  (1)
Datenquelle
  • Artikel: DFG Deutsche Nationallizenzen  (3)
Materialart
Erscheinungszeitraum
  • 1985-1989  (3)
Jahr
Schlagwörter
  • 1
    Digitale Medien
    Digitale Medien
    A simple method for elution of IgA deposits from the skin of patients with dermatitis herpetiformis (1989)
    Springer
    Archives of dermatological research 281 (1989), S. 406-410 
    Details
    ISSN: 1432-069X
    Schlagwort(e): Dermatitis herpetiformis ; Immunoglobulins ; Complement components ; Elution
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary To better understand the role of autoimmunity in the pathogenesis of dermatitis herpetiformis, linear IgA bullous dermatosis or other skin disorders, the antigenic specificity of the immune reactants bound in vivo in the skin must be identified. In order to do so, one must first be able to elute these immune reactants from the skin. We describe here a simple method of eluting not only specifically bound IgG, but also IgA and other immunoglobulins and complement components from skin biopsy material. The method involves cutaneous washing of the entrapped serum proteins in PBS pH 7 and pH 5 buffers followed by specific immunoglobulin elutions at pH 3 and 2. The IgA deposits which could not be removed by this treatment were eluted by a combination of low pH (0.5 M citrate pH 2) and a chaotropic agent (2 M NaCl). The relative concentration of IgA in eluates when quantitated by fluoroimmunoassay were three-to five-fold higher in dermatitis herpetiformis skin biopsy specimens, than in eluates of bullous pemphigoid or normal skin biopsy specimens.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00455326
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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    Artikel (Nationallizenzen)
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  • 2
    Digitale Medien
    Digitale Medien
    Cellular and secreted tumor plasminogen activator: The effects of NaCl (1986)
    Springer
    Cellular and molecular life sciences 42 (1986), S. 433-435 
    Details
    ISSN: 1420-9071
    Schlagwort(e): Plasminogen activator ; NaCl ; metastasis ; MAT 13762 cells
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Plasminogen activator, secreted by metastatic tumor cells, was strongly inhibited in buffer or tissue culture medium containing physiological concentrations of NaCl. Intact cells, however, expressed strong activity under similar conditions. Thus, if plasminogen activator is involved in invasion and metastasis, the cellular activity, acting as an ectoenzyme, may be more important than secreted enzyme under physiological conditions.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02118645
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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    Artikel (Nationallizenzen)
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  • 3
    Digitale Medien
    Digitale Medien
    The determination ofin situ rock thermal properties and the simulation of climate in an underground mine (1986)
    Springer
    Geotechnical and geological engineering 4 (1986), S. 197-216 
    Details
    ISSN: 1573-1529
    Schlagwort(e): In situ rock conductivity ; in situ rock diffusivity ; mine climate simulation ; CLIMSIM ; diesel heat output
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Geologie und Paläontologie
    Notizen: Summary This paper describes the results from a continuing research project started in 1984, which is investigating improved methods for predicting the underground climate in hot mines. The Homestake Gold Mine in South Dakota was the site for testing methods, based on radial heat flow theory, to measurein situ values of the thermal conductivity and diffusivity of rock. The methods used proved to be quite within the capability of mine staff to carry out for themselves. The results were acceptable and verified thatin situ thermal rock properties can differ considerably from laboratory determined values, in this case by a factor of approximately two. A mine climate simulation program, CLIMSIM, was applied to case studies from the Homestake mine using the measured thermal rock parameters. The results showed good correlation between the measured and simulated values.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01560716
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