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  • Articles: DFG German National Licenses  (13)
  • Life Sciences (general)  (6)
  • 15N  (4)
  • Somatic hybrid  (3)
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  • Articles: DFG German National Licenses  (13)
Material
Years
Keywords
  • 1
    Electronic Resource
    Electronic Resource
    Mineralization of soil organic nitrogen solubilized by aqua ammonia fertilizer (1996)
    Springer
    Biology and fertility of soils 23 (1996), S. 93-98 
    Details
    ISSN: 1432-0789
    Keywords: Added nitrogen interaction ; Alkaline-hydrolysing fertilizer ; Aqua ammonia ; Gross N immobilization ; Gross N mineralization ; 15N ; Soluble organic N availability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Organic N solubilized by NH3(aq) was extracted from 15N-labelled or unlabelled soil, concentrated and added to non-extracted soil, which was incubated under aerobic conditions at 27±1°C. Gross N mineralization, gross N immobilization, and nitrification in soils with or without addition of unlabelled soluble organic N were estimated by models based on the dilution of the NH 4 + or NO inf3 sup- pools, which were labelled with 15N at the beginning of incubation. Mineralization of labelled organic N was measured by the appearance of label in the mineral N pool. Although gross N mineralization and gross N immobilization were increased in two soils between day 0 and day 7 following addition of unlabelled organic N solubilized by NH3(aq), there was no increase in net N mineralization. Solubilization of 15N-labelled organic N increased and the 15N enrichment of the soluble organic N decereased as the concentration of NH3(aq) added increased. A constant proportion of approximately one-quarter of the labelled organic N added at different rates to non-extracted soil was recovered in the mineral N pool after an incubation period of 14 days, and the availability ratios calculated from net N mineralization data were 1.1:1 and 2.1:1 for 111 and 186 mg added organic-N kg-1 soil, respectively, indicating that the mineralization of organic N was increased by solubilization.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00335824
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Biological interactions between soil nitrogen and alkaline-hydrolysing nitrogen fertilizers (1995)
    Springer
    Biology and fertility of soils 20 (1995), S. 41-48 
    Details
    ISSN: 1432-0789
    Keywords: Added nitrogen interaction ; Ammonia fixation ; 15N ; Immobilization ; Fertilizer-induced solubilization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Added N interactions were measured in four soil incubated with 15N-labelled urea or diammonium phosphate. The use of biologically active, γ-irradiated, or reinoculated γ-irradiated samples allowed us to separate added N interactions due to chemical and biological processes, and to distinguish real interactions from apparent effects. Real biologically mediated added N interactions were observed in one soil for both fertilizer sources and in three soils amended with urea. These real interactions increased with the N fertilizer rate, but did not differ significantly between N sources. Fertilizer-induced unlabelled organic N in soil extracts declined during incubation in both sterile and non-sterile samples, but the temporal decline was higher in biologically active soil. Changes in fertilizer-induced unlabelled organic N in the extracts of three soils attributed to biological processes were similar to the measured real biologically mediated added N interactions. The results are consistent with the hypothesis that real biologically mediated added N interactions arise from the mineralization of soil organic N solubilized by alkaline-hydrolysing N fertilizers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307839
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Mineralization of soil organic nitrogen solubilized by aqua ammonia fertilizer (1996)
    Springer
    Biology and fertility of soils 23 (1996), S. 93-98 
    Details
    ISSN: 1432-0789
    Keywords: Key words Added nitrogen interaction ; Alkaline-hydrolysing fertilizer ; Aqua ammonia ; Gross N immobilization ; Gross N mineralization ; 15N ; Soluble organic N availability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Organic N solubilized by NH3(aq) was extracted from 15N-labelled or unlabelled soil, concentrated and added to non-extracted soil, which was incubated under aerobic conditions at 27±1°C. Gross N mineralization, gross N immobilization, and nitrification in soils with or without addition of unlabelled soluble organic N were estimated by models based on the dilution of the NH4 + or NO3 – pools, which were labelled with 15N at the beginning of incubation. Mineralization of labelled organic N was measured by the appearance of label in the mineral N pool. Although gross N mineralization and gross N immobilization were increased in two soils between day 0 and day 7 following addition of unlabelled organic N solubilized by NH3(aq), there was no increase in net N mineralization. Solubilization of 15N-labelled organic N increased and the 15N enrichment of the soluble organic N decereased as the concentration of NH3(aq) added increased. A constant proportion of approximately one-quarter of the labelled organic N added at different rates to non-extracted soil was recovered in the mineral N pool after an incubation period of 14 days, and the availability ratios calculated from net N mineralization data were 1.1:1 and 2.1:1 for 111 and 186 mg added organic-N kg–1 soil, respectively, indicating that the mineralization of organic N was increased by solubilization.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00335824
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Somatic hybrids between Brassica juncea (L). Czern. and Diplotaxis harra (Forsk.) Boiss and the generation of backcross progenies (1995)
    Springer
    Theoretical and applied genetics 91 (1995), S. 1167-1172 
    Details
    ISSN: 1432-2242
    Keywords: Protoplast fusion ; Somatic hybrid ; Brassica juncea ; Diplotaxis harra ; Wild gene transfer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract An attempt to transfer genes from droughttolerant Diplotaxis harra, a wild relative of Brassica species, to an elite oil-yielding cultivar, B-85, of mustard (Brassica juncea) was made through protoplast fusion, as the two plant systems are sexually incompatible. By following the standard protocol for PEG-mediated protoplast fusion followed by high pH, high Ca++, DMSO treatment and appropriate cell-culture technique, 16 presumptive somatic hybrid plants could be regenerated. Chromosomal analysis of four such somatic hybrids revealed that three of them were asymmetric. Analysis of morphological characters, meiotic chromosomes, and esterase isoenzyme pattern revealed that all the somatic hybrids were different from each other. Furthermore four chromosomes of each genome could undergo homoeologous pairing at meiosis indicating the possibilities for genetic recombination and chromosomal rearrangements. Irregular distribution of chromosomes at anaphase-II at meiosis has been a consistent feature of these plants. Eventually, pollen of all the somatic hybrids showed complete infertility preventing the recovery of any selfed seed. Nevertheless, ovule fertility of one somatic hybrid was not totally impaired as it had set some seeds upon backcrossing with the B. juncea parent. The esterase isoenzyme banding pattern of 24 individual progeny plants of this backcross provided evidence for their recombinant nature. It was thus confirmed that a transfer of genetic traits from Diplotaxis harra to B. juncea had indeed taken place. Furthermore, it was conceptualised that a transfer of alien genes through the protoplast-fusion technique is primarily possible in situations where meiotic pairing of the chromosomes of the two participating genomes generates recombinant gametocytes which can pass through subsequent filial generations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00223936
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    “Erussica”, the intergeneric fertile somatic hybrid developed through protoplast fusion between Eruca sativa Lam. and Brassica juncea (L.) Czern. (1990)
    Springer
    Theoretical and applied genetics 79 (1990), S. 561-567 
    Details
    ISSN: 1432-2242
    Keywords: Intergeneric somatic hybridization ; Brassica + Eruca ; Somatic hybrid ; Partial somatic hybrid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Hypocotyl calli-derived protoplasts of two cultivars of Brassica juncea (2n=36), a major oil-seed crop, were fused with normal as well as γ-irradiated mesophyll protoplasts of Eruca sativa (2n=22). The irradiation of the Eruca fusion partner increased the plating efficiency as well as the morphogenic potentiality of the fusion products over the normal fusion. Fertile plants could be regenerated from such fusion products. Analysis of 63 out of 181 plants regenerated showed that, indeed, 11 somatic hybrids (2n=58) and 10 partial somatic hybrids (chromosome number ranged between 50 and 56) had been obtained. Pollen viability (0%–82.9%) and seed set (0%–50%) of the hybrids indicated them to be useful for future studies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00226168
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    Intergeneric somatic hybrid production through protoplast fusion between Brassica juncea and Diplotaxis muralis (1988)
    Springer
    Theoretical and applied genetics 76 (1988), S. 915-922 
    Details
    ISSN: 1432-2242
    Keywords: Intergeneric somatic hybridisation ; Brassica ; Diplotaxis ; Somatic hybrid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The need to transfer genetic traits from Diplotaxis muralis (2n=42) to Brassica juncea (2n=36), a major oil seed crop of the tropical world, was realised. Since the two plant types are sexually incompatible, attempts were made to evolve parasexual hybrids as the result of protoplast fusion. Protoplasts of hypocotyl-derived calli of two cultivars of B. juncea were fused with normal and γ-irradiated mesophyll protoplasts of Diplotaxis muralis. Regeneration of 110 plants from the fused products was successfully achieved. Upon analysis of some of them, we realised that true somatic hybrids and partial somatic hybrids had been generated. Thus the primary goal of evolving intergeneric hybridisation products between these two plant types was fulfilled.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00273681
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    Paper (German National Licenses)
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  • 7
    Electronic Resource
    Electronic Resource
    Solubilization of soil organic N by alkaline-hydrolysing N fertilizers (1994)
    Springer
    Nutrient cycling in agroecosystems 38 (1994), S. 131-139 
    Details
    ISSN: 1573-0867
    Keywords: aqua ammonia ; di-ammonium phosphate ; gamma-irradiated soil ; 15N ; organic matter solubility ; urea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Interactions between15N-labelled fertilizers applied at concentrations representative of the fertilizer microsite and the solubility of the nitrogenous component of soil organic matter were investigated in laboratory experiments. Soil organic N was solubilized in aγ-irradiated soil due to addition of NH3(aq), and the fertilizer-induced loss of unlabelled total N in the extracted soil (ΔTUs) increased with increasing N fertilizer concentration and soil pH. ΔTUs was linearly correlated with ammoniacal-N concentration and the pH of the fertilized soil within the range of 7.5-10 (r = 0.94). Total organic N in the soil extract (OTe) increased rapidly up to day 14 following addition of 2000 mg urea-N kg−1 soil, but was then stable up to day 28. OTe of a range of soils increased from between 5 and 148 to between 15 and 368 mg N kg−1 soil after application of 1045 mg NH3-N kg−1 soil. While up to 25% of the organic N was solubilized by the fertilizer in nine soils, the change in total organic N in the extracts (ΔOTe) of three soils was not significant. The highest ΔOTe of 399 mg N kg−1 soil (35.4% of soil organic N) was measured after application of 2000 mg NH3-N kg−1 soil. pH and ΔOTe decreased in the order of NH3(aq) 〉 urea 〉 di-ammonium phosphate 〉 ammonium sulphate at equivalent rates of N addition. A negative ΔOTe was measured following application of ammonium sulphate. ΔOTe was correlated with the pH of the fertilized soil but not ammoniacal-N concentration for different N fertilizer sources.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00748773
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  • 8
    Electronic Resource
    Electronic Resource
    Influence of antibiotics and trace salts on lysine production by arthrobacter globiformis (1985)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 5 (1985), S. 215-218 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: A hydrocarbon utilizing strain of Arthrobacter globiformis Lb isolated from local soil has been found to yield lysine 3.4 g l-1, keeping the medium optimal for pH, C- and N-sources. Addition of antibiotics and micronutrients to that optimal media stimulated cell growth and enhanced lysine yield.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370050223
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    Paper (German National Licenses)
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  • 9
    Electronic Resource
    Electronic Resource
    Extracellular amylase synthesis by Bacillus globisporus BH-1b (1994)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 14 (1994), S. 97-104 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Despite the prevalence of starch-hydrolyzing enzymes in bacilli, relatively few have been studied in detail. In an attempt to isolate an effective α-amylase-producing strain, Bacillus globisporus BH-1b has been isolated. The strain requires few nutritional supplements and shows induction in the presence of galactose. 2% potassium nitrate and pH 7.2 emerged as optimum for the fermentation medium. The durability of the enzyme has also been tested at a low pH and a high temperature.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370140116
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  • 10
    Electronic Resource
    Electronic Resource
    Activity of diaminopimelic acid decarboxylase in bacteria producing lysine (1983)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 3 (1983), S. 217-220 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Activity of DAP-decarboxylase in lysine overproducing strains of Micrococcus luteus, M. varians and Arthrobacter globiformisLb reached the highest level during the end of the exponetial phase of growth and remained at the same high level during the stationary phase of growth when major bulk of lysine was accumulated. In comparison in a lysine non-producing strain of Arthrobacter globiformis I 4 the activity of the same enzyme was low. DAP-decarboxylase of these three lysine overproducers has two specialities, persistence during the stationary phase and insensivity to repression by exogenous lysine.
    Additional Material: 2 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370030303
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