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  • Articles: DFG German National Licenses  (13)
  • Cell & Developmental Biology  (12)
  • Anorectal manometry  (1)
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  • Articles: DFG German National Licenses  (13)
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Keywords
  • 1
    Electronic Resource
    Electronic Resource
    Transducer manometry and the effect of body position on anal canal pressures (1990)
    Springer
    Diseases of the colon & rectum 33 (1990), S. 469-475 
    Details
    ISSN: 1530-0358
    Keywords: Anorectal manometry ; Transducer probe ; Perfused probe ; Body position
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Anal canal manometry is performed conventionally with balloons, sleeves, perfused or nonperfused open-tipped catheters, or with multiport probes. The authors constructed a new manometer with four transducers embedded in a probe (15 mm outside diameter) and oriented radially, 90
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02052140
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Detection and location of amphiregulin and Cripto-1 expression in the developing postnatal mouse mammary gland (1995)
    New York, NY [u.a.] : Wiley-Blackwell
    Molecular Reproduction and Development 41 (1995), S. 277-286 
    Details
    ISSN: 1040-452X
    Keywords: EGF-like ligands ; Postnatal development ; RT-PCR ; Immunocytochemistry ; Immunoblotting ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Amphiregulin (Ar) and Cripto-1 (Cr-1) are growth promoting peptides that share amino acid sequence homology with epidermal growth factor (EGF). The present study examined Ar and Cr-1 mRNA and protein expression during various stages of C57BL/6 mouse mammary morphogenesis. Reverse transciption-polymerase chain reaction (RT-PCR) was used to detect transcripts for Ar and Cr-1 at all stages of mammary development. Immunocytochemical (ICC) localization demonstrated that in virgin 4-week to mature 12-week-old mouse fourth inguinal mammary gland, Ar and Cr-1 are expressed in the stromal cells, luminal epithelial cells, and myoepithelial cells of the branching ducts. Ar, and to lesser extent Cr-1, were also found in the epithelial cap cells and in the luminal epithelial cells of the advancing terminal end bud (TEB) from virgin 4-week and 6-week-old mice. Western blot analysis demonstrated that both Ar (28 and 26 kDa) and Cr-1 (90, 67, 56, and 21 kDa) proteins are expressed in virgin, 13.5 day midpregnant and in the 14 day lactating mammary gland. In addition, Ar and Cr-1 are associated with developing alveolar structures as determined by ICC. These results imply that together with EGF and transforming growth factor alpha (TGFα), Ar and Cr-1 may play salient roles as modifiers in the morphogenesis and differentiation of the mammary gland. © 1995 Wiley-Liss, Inc.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1080410302
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Colony formation in agar by adult bone marrow multipotential hemopoietic cells (1980)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 103 (1980), S. 371-383 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Erythroid colony formation in agar cultures of CBA bone marrow cells was stimulated by the addition of pokeweed mitogen-stimulated spleen conditioned medium (SCM). Optimal colony numbers were obtained when cultures contained 20% fetal calf serum and concentrated spleen conditioned medium. By 7 days of incubation, large burst or unicentric erythroid colonies occurred at a maximum frequency of 40-50 per 105 bone marrow cells. In CBA mice the cells forming erythroid colonies were also present in the spleen, peripheral blood, and within individual spleen colonies. A marked strain variation was noted with CBA mice having the highest levels of erythroid colony-forming cells.In CBA mice erythroid colony-forming cells were mainly non-cycling (12.5% reduction in colony numbers after incubation with hydroxyurea or 3H-thymidine). Erythroid colony-forming cells sedimented with a peak of 4.5 mm/hr, compared with CFU-S, which sedimented at 4.25 mm/hr. The addition of erythropoietin (up to 4 units) to cultures containing SCM did not alter the number or degree of hemoglobinisation of erythroid colonies.Analysis of the total number of erythroid colony-forming cells and CFU-S in 90 individual spleen colonies gave a correlation coefficient of r = 0.93 for these two cell types.In addition to benzidine-positive erythroid cells, up to 40% of the colonies contained, in addition, varying proportions of neutrophils, macrophages, eosinophils, and megakaryocytes. Taken together with the close correlation between the numbers of CFU-S in different adult hemopoietic tissues, including individual spleen colonies, the data indicate that the erythroid colony-forming cells expressing multiple hemopoietic differentiation are members of the hemopoietic multipotential stem cell compartment.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041030302
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Production by spleen and lymph node cells of conditioned medium with erythroid and other hemopoietic colony-stimulating activityThis work was supported by the Carden Fellowship Fund of the Anti-Cancer Council of Victoria, the National Health and Medical Research Council, Canberra and the National Cancer Institute. Bethesda, Contract NOI-CB-74148. (1978)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 96 (1978), S. 31-42 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Pokeweed mitogen-stimulated suspension cultures of mouse spleen cells produced conditioned medium able to stimulate granulocyte-macrophage, eosinophil and megakaryocyte colony formation in agar cultures of C57BL marrow cells and granulocyte-macrophage and erythroid colony formation in agar cultures of CBA fetal liver cells. Medium conditioned by other mouse tissues stimulated only granulocyte-macrophage colony formation and this activity was not increased by the addition of pokeweed mitogen. Spleen cells stimulated by mixed leucocyte culture or concanavalin-A had a weak capacity to stimulate erythroid colony formation.Production of the factors stimulating the four types of hemopoiesis was T-lymphocyte dependent and nu/nu spleen cells were inactive. Factor production was also dependent on adherent cells but evidence from rat-mouse combinations suggested that the T-lymphocytes actually produced the active factors.Production of the four types of colony stimulating factors was radiosensitive (D0120-238 rads) and spleen cell populations of lighter buoyant density than 1.075 g/cm3 and sedimenting at 3.5-5.0 mm/hour were able to produce active conditioned media. Fractionation experiments failed to segregate spleen populations able to produce only one of the four stimulating factors.Production of factors stimulating hemopoiesis by mitogen-stimulated lymphoid populations could be a process contributing to the control of hemopoiesis in vivo.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1040960105
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  • 5
    Electronic Resource
    Electronic Resource
    Factors affecting the generation of mast cells from multipotential colony-forming cells (1984)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 121 (1984), S. 167-173 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The cells responsible for the long-term in vitro generation of murine mast cells have been examined. Sequential analysis of all colony types obtained from cultures of spleen or bone marrow cells showed that only colonies derived from multipotential cells (mixed-erythroid colonies) or mast cell progenitors, contained cells responsible for mast cell generation in liquid cultures. Primary colony growth and subsequent maintenance of mast cells in liquid cultures was dependent upon pokeweed mitogen-stimulated spleen cell-conditioned medium (SCM). Mixed-erythroid colonies from 14-day cultures of spleen cells had the greatest capacity for mast cell generation. Analysis by clone splitting and transfer to high (20%) and low (2.5%) concentrations of SCM showed that the concentration of SCM used in either the primary colony culture or subsequent liquid culture phase altered both the proliferative capacity of the mast cells generated and the frequency of mast cell progenitors within individual mixed-erythroid colonies. Thus, mixed-erythroid colonies stimulated with 2.5% SCM contained the highest proportion of mast cell progenitors (34% of colonies) and when stimulated with 20% SCM, approximately fourfold higher numbers of mast cells were produced at weekly intervals from liquid cultures maintained in 2.5% SCM compared to parallel liquid cultures containing 20% SCM. These studies confirm the hemopoietic origin of mast cells and demonstrate that a factor(s) in SCM is able to modulate their proliferative potential.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041210121
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  • 6
    Electronic Resource
    Electronic Resource
    Nature of cells forming erythroid colonies in agar after stimulation by spleen conditioned mediumThis work was supported by the Carden Fellowship Fund of the Anti-Cancer Council of Victoria, The National Health and Medical Research Council, Camberra and the National Cancer Institute, Bethesda, Contract NOI-CB-33854. (1978)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 94 (1978), S. 243-252 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Erythroid colony formation in agar cultures of CBA cells was stimulated by the addition of pokeweed mitogen-stimulated C57BL spleen conditioned medium. Both 48-hour colonies (“48-hour benzidine-positive aggregates”) and day 7 large burst or unicentric erythroid colonies (“erythroid colonies”) developed, together with many neutrophil and/or macrophage colonies.In CBA mice, the cells forming erythroid colonies occurred with maximum frequency (650/105 cells) in 10- to 11-day-old yolk sac and fetal liver but were present also in fetal blood, spleen and bone marrow. The frequency of these cells fell sharply with increasing age and only occasional cells (2/105 cells) were present in adult marrow. A marked strain variation was noted, CBA mice having the highest levels of erythroid colony-forming cells.The erythroid colony-forming cells in 12-day CBA fetal liver were radiosensitive (Do 110-125 rads), mainly in cycle and were non-adherent, light density, cells sedimenting with a peak velocity of 6-9 mm/hr. These properties are similar to those of other hemopoietic progenitor cells in fetal tissues. The relationship of these apparently erythropoietin-independent erythroid colony-forming cells to those forming similar colonies after stimulation by erythropoietin remains to be determined.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1040940302
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  • 7
    Electronic Resource
    Electronic Resource
    Interactions between purified GM-CSF, purified erythropoietin and spleen conditioned medium on hempoietic colony formation in vitroThis work was supported by the Carden Fellowship Fund of the Anti-Cancer Council of Victoria, the National Health and Medical Research Council, Canberra and the National Cancer Institute, Washington, Contract No. NOIC74148. (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 99 (1979), S. 159-174 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Preincubation of C57BL adult marrow cells or CBA fetal liver cells with a 250-fold excess concentration of purified GM-CSF failed to reduce the frequency of cells forming eosinophil, megakaryocyte or erythroid colonies in subsequent agar cultures. When excess concentrations of purified GM-CSF were added to agar cultures stimulated by pokeweed mitogen-stimulated spleen conditioned medium (SCM), no reduction was observed in the frequency of eosinophil, megakaryocyte or erythroid colonies. Addition of 4 units of purified erythropoietin (EPO) to cultures of fetal liver or adult marrow cells stimulated by SCM increased the number of erythroid colonies but did not reduce the number of non-erythroid colonies or the non-erythroid content of mixed erythroid colonies.Although neither GM-CSF nor EPO alone was able to stimulate erythroid colony formation in agar cultures of fetal liver cells, small numbers of large erythroid colonies were stimulated to develop in cultures containing both purified regulators. Purified GM-CSF was also able to support the survival in vitro of a small proportion of erythroid colony-forming cells in fetal liver populations cultured initially in the absence of SCM and the survival of some eosinophil and megakaryocyte colony-forming cells in similar cultures of adult marrow cells.The results do not support the hypothesis that GM-CSF and EPO compete for a common pool of uncommitted progenitor cells. On the contrary, the data indicate that GM-CSF and EPO are able to collaborate in stimulating the proliferation of some erythropoietic cells. Furthermore, purified GM-CSF appears to be able to support temporarily the survival and/or initial proliferation of at least some cells forming erythroid, eosinophil and megakaryocyte colonies, even though GM-CSF is unable to stimulate the formation of colonies of these types.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1040990202
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  • 8
    Electronic Resource
    Electronic Resource
    Characterization of two populations of CFU-S fractionated from mouse fetal liver by fluorescence-activated cell sorting (1984)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 118 (1984), S. 45-52 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The properties of spleen colony forming units (CFU-S) contained in two murine fetal liver cell populations (pre-colony-forming cell (CFC) and CFC fractions) obtained after fluorescence-activated cell sorting fractionation on the basis of pokeweed mitogen binding have been analyzed. Individual spleen colonies generated by both populations were examined to determine cellularity, differential cell morphology, as well as CFU-S and in vitro CFC content. Spleen colonies from both populations contained approximately equal numbers of cells 7 days after transplantation; at 12 days, however, CFU-S from the pre-CFC fraction produced four times as many cells per colony than colonies from the CFC fraction. Although the frequency of spleen colonies containing CFU-S was similar, in comparison to the CFC fraction, spleen colonies obtained from the pre-CFC fraction contained higher absolute numbers of CFU-S. This was most evident with CFU-S assayed 12 days after transplantation of 12-day spleen colony cells (pre-CFC fraction, 84% of colonies contained CFU-S, mean of 527 per colony; CFC fraction, 77% of colonies contained CFU-S, mean of 41 per colony). Similarly, the in vitro CFC content of 7- or 12-day spleen colonies was higher in colonies produced by pre-CFC fraction CFU-S. No significant differences were observed in either the proportion of CFU-S in S-phase or the morphology of spleen colonies produced by either cell population.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041180110
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  • 9
    Electronic Resource
    Electronic Resource
    In vitro actions on hemopoietic cells of recombinant murine GM-CSF purified after production in Escherichia coli: Comparison with purified native GM-CSF (1986)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 128 (1986), S. 421-431 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Recombinant murine GM-CSF produced in Escherichia coli was purified to homogeneity and tested in parallel with purified native GM-CSF. Both recombinant and native GM-CSF stimulated granulocyte and/or macrophage colony formation by adult and fetalmouse progenitor cells, and with adult marrow cells the specific activity of the recombinant GM-CSF (25 × 108 U/mg) was similar to that of the native form (15 × 108 U/mg). At high concentrations (〉 200 U/ml), both forms of GM-CSF also stimulated eosinophil colony formation by adult marrow cells and, at very high concentrations (〉 800 U/ml), megakaryocyte and some erythroid and mixed-erythroid colony formation. Recombinant GM-CSF was as effective in stimulating the proliferation of the GM-CSF-dependent cell line FD as the native molecule. Both recombinant and native GM-CSF were able to induce partial differentiation in colonies of WEHI-3B myeloid leukemic cells. Recombinant GM-CSF competed effectively for the binding of 125l-labeled native GM-CSF to hemopoietic cells, and anti-serum to recombinant GM-CSF also neutralized the biological activity of native GM-CSF. The bacterially synthesized GM-CSF was a slightly more effective stimulus for megakayocyte colony formation than then native molecule. The demonstration that purified bacterially synthesized GM-CSF is biologically active in vitro now permits studies to be undertaken on the in vivo effects of this material.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041280311
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  • 10
    Electronic Resource
    Electronic Resource
    Macrophage cell lines transformed by the malignant histiocytosis sarcoma virus: Increase of CSF receptors suggests a model for transformation (1987)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 132 (1987), S. 22-32 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The malignant histiocytosis sarcoma virus (MHSV) contains Ha-v-ras-related oncogenic sequences and rapidly transforms myeloid cells in vivo and in vitro. Myeloid cell lines can be derived which do not require growth factor for continued proliferation. We initiated this work to define the process of transformation leading to autonomy of cell growth in transformed myeloid cells. Five established cell lines were examined. All express macrophagespecific cell-surface antigens and exhibit several other properties typical for mature macrophages. Growth properties, growth factor release, and growth factor receptor presentation were examined: Release of growth factors is not a consistent feature. All cell lines show cell-density-independent colony formation and do not release self-stimulating factors, thus excluding autocrine stimulation as a model leading to transformation. All cell lines express unusually high levels of granulocyte-macrophage (GM)-and multi-CSF receptors and, except for one M-CSF receptors. The high increase in GM-CSF and other growth factor receptors may be causally related to the transformed state of the cells. MHSV can be used as a tool to easily derive cell lines of the macrophage pathway as a model to study myeloid transformation, differentiation, and macrophage function.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041320104
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