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  • Articles: DFG German National Licenses  (4)
  • Stiff-man syndrome  (2)
  • B-cell volume  (1)
  • Bezieziehungen zu psychoorganischen Veränderungen  (1)
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  • Articles: DFG German National Licenses  (4)
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  • 1
    ISSN: 1432-0428
    Keywords: Pregnancy ; B-cell volume ; insulin ; Wistar rats ; streptozotocin administration ; islets ; DNA synthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The effect of pregnancy on pancreatic insulin content and relative B-cell volume has been studied in normoglycaemic Wistar rats treated with streptozotocin 14 days before mating. A single intravenous injection of streptozotocin (30 mg/kg body weight) caused a significant reduction of pancreatic insulin content and B-cell volume. The islet insulin content was 60% of control values. However, pregnancy-associated adaptation was preserved in these streptozotocin-treated animals. Plasma insulin levels, pancreatic insulin and B-cell volume were significantly enhanced compared with non-pregnant rats investigated on the same date. The incorporation of [3H]-thymidine into islets from pregnant rats (day 10.5) was higher than that in islets isolated from non-pregnant animals. After delivery insulin content and B-cell volume returned to pre-pregnant values. Also during a longer period after streptozotocin treatment (156 days), no measurable enhancement of B-cell volume and pancreatic insulin content was observed indicating the unresponsiveness of residual B cells to compensate spontaneously for the loss despite persisting normoglycaemia.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    European archives of psychiatry and clinical neuroscience 223 (1977), S. 131-138 
    ISSN: 1433-8491
    Keywords: Sleep EEG ; Circulation Disturbances ; Correlation to Psycho-organic Impairment ; Schlaf-EEG ; cerebrale Durchblutungsstörungen ; Bezieziehungen zu psychoorganischen Veränderungen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Bei 30 bewußtseinsklaren Patienten (24 Männer, 6 Frauen) im Alter von 30–74 Jahren mit ischämischen Großhirnhemisphärenläsionen wurden durchschnittlich 45 Tage nach Krankheitsbeginn polygraphische Nachtschlafregistrierungen durchgeführt. Die Schlaf-EEG-Veränderungen waren gekennzeichnet durch eine Verlängerung der Einschlaf- und Wachzeiten sowie eine Abnahme des tiefen synchronen Schlafes. Korrelationsstatistische Untersuchungen zeigen, daß ältere Patienten längere Wachzeiten haben, ebenso wie Kranke mit hohen komplexen Reaktionszeiten und einer euphorischen Stimmungslage, die als Ausdruck einer hirnorganischen Leistungsbeeinträchtigung interpretiert werden können. Demgegenüber tritt die Schwere des klinisch-neurologischen Syndroms für Art und Schwere der Schlafstörung zurück.
    Notes: Summary In 30 conscious patients (24 men, 6 women) aged 30–74 years suffering from ischemic lesions in the cerebral hemispheres, polygraphic night sleep recordings were performed about 45 days after the beginning of the illness. The alterations in sleep were characterized by a delay in the onset of sleep, prolonged waking periods, and a reduction of deep synchronous sleep. Statistical analysis revealed a relationship between prolonged waking time with higher age as well as with high complex reaction time and euphoric state of mind, which can be considered as a sign of psycho-organic impairment. In contrast thereto, the degree of the neurological symptomatology is of less importance for the kind and extent of the sleep disturbances.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-5233
    Keywords: Murine monoclonal glutamate decarboxylase antibodies ; Autoantibodies ; Type 1 diabetes mellitus ; Stiff-man syndrome
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract To study the immune response to glutamic acid decarboxylase (GAD) in insulin-dependent diabetes mellitus, monoclonal GAD antibodies after fusion of splenocytes from a nondiabetes-susceptible BALB/c mouse immunized with human recombinant GAD65 were generated. Of the 44 monoclonals, 35 are specific for the GAD65 isoform, whereas 9 also react with GAD67. Some 37 monoclonals, including all GAD65/67 reactive antibodies, react with GAD by Western blot analysis. The remaining 7 GAD65 monoclonals bind GAD only in an immunoprecipitation assay, which implies that they target epitopes dependent on the conformation of the GAD molecule. The125I-GAD binding of the GAD65 monoclonals reactive on Western blotting was significantly diminished by all 3 sera from Stiff-man syndrome patients but only by 3/30 (10%) sera from type 1 diabetic patients. In contrast, the 7 monoclonal antibodies reactive with a conformation-dependent GAD epitope were competitive with 83% of GAD-autoantibody-positive sera from these diabetic patients. Using chimeric GAD65/67 proteins, the epitope region targeted by these monoclonals was mapped to the middle of GAD65 (amino acids 221–442). This central conformation-dependent GAD region was also targeted by sera from patients with type 1 diabetes. In conclusion, our data show that evne after common immunization of a nondiabetes-susceptible mouse strain, monoclonals were obtained which preferentially react with the GAD65 linear amino-terminus (amino acids 4–17) and a conformation-dependent region located in the middle of GAD targeted by autoantibodies, indicating that this GAD region is not restricted to the autoimmune response associated with the Stiff-man syndrome and the bete-cell destruction in type 1 diabetes mellitus.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1432-5233
    Keywords: Key words Murine monoclonal glutamate decarboxylase antibodies ; Autoantibodies ; Type 1 diabetes mellitus ; Stiff-man syndrome
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract To study the immune response to glutamic acid decarboxylase (GAD) in insulin-dependent diabetes mellitus, monoclonal GAD antibodies after fusion of splenocytes from a nondiabetes-susceptible BALB/c mouse immunized with human recombinant GAD65 were generated. Of the 44 monoclonals, 35 are specific for the GAD65 isoform, whereas 9 also react with GAD67. Some 37 monoclonals, including all GAD65/67 reactive antibodies, react with GAD by Western blot analysis. The remaining 7 GAD65 monoclonals bind GAD only in an immunoprecipitation assay, which implies that they target epitopes dependent on the conformation of the GAD molecule. The 125I-GAD binding of the GAD65 monoclonals reactive on Western blotting was significantly diminished by all 3 sera from Stiff-man syndrome patients but only by 3/30 (10%) sera from type 1 diabetic patients. In contrast, the 7 monoclonal antibodies reactive with a conformation-dependent GAD epitope were competitive with 83% of GAD-autoantibody-positive sera from these diabetic patients. Using chimeric GAD65/67 proteins, the epitope region targeted by these monoclonals was mapped to the middle of GAD65 (amino acids 221–442). This central conformation-dependent GAD region was also targeted by sera from patients with type 1 diabetes. In conclusion, our data show that even after common immunization of a nondiabetes-susceptible mouse strain, monoclonals were obtained which preferentially react with the GAD65 linear amino-terminus (amino acids 4–17) and a conformation-dependent region located in the middle of GAD targeted by autoantibodies, indicating that this GAD region is not restricted to the autoimmune response associated with the Stiff-man syndrome and the beta-cell destruction in type 1 diabetes mellitus.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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