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Expression of multidrug-resistance-associated protein gene in human soft-tissue sarcomas (1996)

Oda, Yoshinao ; Schneider-Stock, Regine ; Ryś, Janusz ; [et al.]

Springer

Journal of cancer research and clinical oncology 122 (1996), S. 161-165

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ISSN: 1432-1335

Keywords: Soft-tissue sarcoma ; MRP mRNA ; Multidrug resistance ; MDR1 ; RT-PCR

Source: Springer Online Journal Archives 1860-2000

Topics: Medicine

Notes: Abstract We examined the mRNA expression of the multidrug-resistance-associated protein gene (MRP) in soft-tissue sarcomas and compared it with the expression of the multidrug resistance gene (MDR1), using the reverse transcriptase/polymerase chain reaction. We investigated 39 samples from 33 cases of soft-tissue sarcomas (11 liposarcomas, 9 malignant fibrous histiocytomas, 6 leiomyosarcomas, 4 malignant schwannomas, 3 fibrosarcomas, 3 synovial sarcomas, and 3 epithelioid sarcomas) and 7 benign softtissue tumors. All samples were obtained prior to chemotherapy. An expression ofMRP mRNA was noted in 56% of soft-tissue sarcoma specimens. The co-expression ofMRP andMDR1 was recognized in 15 samples (38%) (5/11 liposarcomas, 5/9 malignant fibrous histiocytomas, 3/6 leiomyosarcomas, 2/3 fibrosarcomas) and significantly correlated with histological grade (P=0.0165). A positive and significant correlation was found betweenMRP andMDR1 expression in soft-tissue sarcomas (P=0.0013). In benign soft-tissue tumors, 1 chemodectoma and 1 neurothekeoma showed lowMRP expression; however, no case showed co-expression ofMRP andMDR1.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF01366956

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Improved detection of P53 mutations in soft tissue tumors using new gel composition for automated nonradioactive analysis of single-strand conformation polymorphism (1997)

Schneider-Stock, Regine ; Haeckel, Carsten ; Radig, Kathrin ; [et al.]

Weinheim : Wiley-Blackwell

Electrophoresis 18 (1997), S. 2849-2851

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ISSN: 0173-0835

Keywords: Polyacrylamide gel electrophoresis ; Single-strand conformation polymorphism ; Automated sequencer ; p53 gene ; Chemistry ; Biochemistry and Biotechnology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology , Chemistry and Pharmacology

Notes: We report a new nonradioactive method to detect sequence changes, including single-base substitutions through shifts in electrophoretic mobility using an automated fluorescence sequencer (ALFexpress, Pharmacia, Biotech) connected to external cooling equipment. Single strands were identified by incorporation of fluorescein-labeled primers during amplification and subsequent laser detection at the bottom of the gel. The amplified polymerase chain reaction (PCR) products were heat-denatured and loaded onto a polyacrylamide gel under nondenaturing conditions and strict control of constant low temperature. Peak shifts in the fluorogram indicated mutations. A novel gel composition improved the detection rate for mutations considerably. Automatic analysis of single-strand conformation polymorphism (SSCP) gels saves time and costs, and is highly reproducible. The method was applied for mutation screening in exon 7 of the p53 tumor suppressor gene in DNA of freshly frozen soft tissue tumors. The mutation spectrum and frequency in exon 7 of the p53 gene are discussed with respect to oncogenesis in soft tissue sarcomas.

Additional Material: 2 Ill.