ISSN:
1432-1335
Keywords:
Soft-tissue sarcoma
;
MRP mRNA
;
Multidrug resistance
;
MDR1
;
RT-PCR
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract We examined the mRNA expression of the multidrug-resistance-associated protein gene (MRP) in soft-tissue sarcomas and compared it with the expression of the multidrug resistance gene (MDR1), using the reverse transcriptase/polymerase chain reaction. We investigated 39 samples from 33 cases of soft-tissue sarcomas (11 liposarcomas, 9 malignant fibrous histiocytomas, 6 leiomyosarcomas, 4 malignant schwannomas, 3 fibrosarcomas, 3 synovial sarcomas, and 3 epithelioid sarcomas) and 7 benign softtissue tumors. All samples were obtained prior to chemotherapy. An expression ofMRP mRNA was noted in 56% of soft-tissue sarcoma specimens. The co-expression ofMRP andMDR1 was recognized in 15 samples (38%) (5/11 liposarcomas, 5/9 malignant fibrous histiocytomas, 3/6 leiomyosarcomas, 2/3 fibrosarcomas) and significantly correlated with histological grade (P=0.0165). A positive and significant correlation was found betweenMRP andMDR1 expression in soft-tissue sarcomas (P=0.0013). In benign soft-tissue tumors, 1 chemodectoma and 1 neurothekeoma showed lowMRP expression; however, no case showed co-expression ofMRP andMDR1.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01366956
