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  • 1
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A major limitation of studies on the parvovirus B 19, a causative agent of transient aplastic crisis, has been the absence of appropriate cell lines permissive for the virus. In the present study, a human erythroid leukemia cell line (JK-1) was shown to support B 19 virus DNA replication in vitro. Forty-eight hours after virus inoculation of JK-1 liquid cell cultures, the average number of B 19 genome copies was estimated at 3,000 per cell by DNA dot blot analysis. The addition of erythropoietin increased B 19 copy number to 10,000 per cell. The presence of replicative forms of the B 19 virus genome was demonstrated by Southern blot analysis. Although persistent infection of B 19 virus was not observed in JK-1 cells, this culture system will be of value in elucidating the molecular basis of the erythroid specificity of parvovirus B 19.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 70 (1991), S. 6056-6058 
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: Co81Cr19/Al multilayered films with thicknesses of Co81Cr19 layers in the range of 46–170 A(ring) and Al ones of 7 A(ring) have been prepared on glass-slide substrate at room temperature by using the facing targets sputtering (FTS) apparatus. The dependence of the magnetic and crystallographic characteristics on the thickness of Co-Cr layers have been investigated. The magnetoresistance effect of the specimen films have been also examined. The intrinsic Co81Cr19 layers were composed of hcp phase crystallites with well orientation of the c axis normal to the film plane. Both the crystallinity of Co81Cr19 layers was improved and the d spacing between the c planes was expanded by using the Al interlayers as ultrathin as 7 A(ring). The easy direction of magnetization in the films was perpendicular to the film plane. The saturation magnetization, the coercivity, the in-plane squareness ratio and the anisotropy field depended strongly on the thickness of Co81Cr19 layer. On the other hand, the magnetoresistivity ratio was negative and its absolute value was as small as 0.13%.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: Amorphous CoZrNb films have been deposited by magnetron sputtering which can greatly improve the utilization efficiency of a magnetic alloy target with high permeability. The saturation magnetization 4πMs of the CoZrNb films was about 14 kG. The easy and hard axes of magnetization of the films were orthogonal to each other in the film plane. The coercivity Hc in the easy and hard directions decreased from 0.9 to 0.2 Oe after annealing in a rotating dc magnetic field HA. However, Hc increased drastically to 20 Oe by heating at the annealing temperature TA of 400 °C. With increasing TA and HA, the anisotropy field Hk gradually decreased from 12 to 1 Oe and the resistivity ρ also decreased from 200 to 150 μΩ cm. Consequently, it was found that Hc and ρ depended strongly on TA; and that Hk had definite relationships with both TA and HA.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 88 (2000), S. 6444-6450 
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: We applied photoacoustic (PA), photoluminescence (PL), photoluminescence excitation (PLE), and atomic force microscopy (AFM) techniques on porous silicon (PS) layers to study the influence of chemical etching by low-concentration hydrofluoric acid. The chemical etching reveals the formation of PS layers of small dimensions by AFM observations, indicating the possibility of a strong quantum confinement effect. PA spectroscopy is useful to obtain the optical absorption characteristic for strongly scattering media such as PS and it helps to confirm the above speculation by indicating the blueshift of the fundamental absorption edge for the PS layer with chemical etching. PL spectroscopy also confirms the possibility of a quantum confinement effect by revealing the strong intensity and blueshift for the PS layer with chemical etching. PLE measurements suggest that the site for the radiative processes is different from that for the recombination of carriers and the PL of PS layers were dominated only by small crystallites in various size distributions. © 2000 American Institute of Physics.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    [S.l.] : American Institute of Physics (AIP)
    Physics of Plasmas 9 (2002), S. 171-176 
    ISSN: 1089-7674
    Source: AIP Digital Archive
    Topics: Physics
    Notes: An n=1 mode global motion on a field-reversed configuration (FRC) plasma is observed by means of a newly developed optical system. The deviation of the FRC from the coil axis reaches 20%–40% of the plasma radius. In order to push back the FRC to the equilibrium position, a multipole field (quadrupole or hexapole field) is applied. The n=1 motion can be easily controlled by the quadrupole field, the critical field strength of which is required to be about 15% of the confinement field. It is found that the n=2 rotational instability can also be stabilized by strength of the same order. The critical strength for the n=1 motion is theoretically obtained from a model such that the driving energy of the motion given at the formation phase balances with the work done by the multipole field. The theoretical estimation agrees within a factor of 2 with the experimental results. © 2002 American Institute of Physics.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    [S.l.] : American Institute of Physics (AIP)
    Review of Scientific Instruments 61 (1990), S. 3524-3527 
    ISSN: 1089-7623
    Source: AIP Digital Archive
    Topics: Physics , Electrical Engineering, Measurement and Control Technology
    Notes: A broadband reflectometric system in O-mode operation has been developed for the density profile determination, MHD and turbulent fluctuation measurement, and evaluation of the particle diffusion coefficient in the JT-60 tokamak. For the profile determination, full Ka-Q band frequencies of BWOs have been swept in 750 μs, in order to minimize the effect of doppler shift due to the fluctuation of reflection layers. The heterodyne fixed-frequency reflectometer has unveiled its effectiveness to probe MHD activities, diagnosing the m=1 tearing mode oscillations. It has also enabled the observation of the dramatic suppression of edge plasma density fluctuations at the L- to H-mode transition in the LHCD limiter plasma. Furthermore, the propagation delay of density pulses has been observed by different fixed-frequency channels during a series of sawteeth, from which the particle diffusion coefficient was evaluated, with a newly proposed method applicable to fully diffusive plasmas.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: Transconductance oscillations were observed for silicon-on-insulator metal–oxide–semiconductor field-effect transistors with 50 nm channel length and 6 nm Si-layer thickness in the temperature range of 39–50 K. By investigating the temperature dependence of the oscillations it was found that the oscillations were caused by two reasons. One reason is the roughness at the Si/insulator interface responsible for the low-gate-voltage oscillations. The roughness results in different thicknesses of the Si layer along the channel, causing different quantized energy levels, which act as barriers for carriers moving in the channel. The other reason is the tunneling through the potential barrier at the p/n junctions between the contacts and the channel, which is responsible for the high-gate-voltage oscillations. © 2002 American Institute of Physics.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Science, Ltd
    British journal of dermatology 146 (2002), S. 0 
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Summary  Background  We have previously reported that several selective protein kinase C (PKC) inhibitors, including procyanidin B-2, promote hair epithelial cell growth and stimulate anagen induction.Objectives  We discuss the hypothesis that the hair-growing activity of procyanidin B-2 is related to its downregulation or inhibition of translocation of PKC isozymes in hair epithelial cells.Methods  We examined the effect of procyanidin B-2 on the expression of PKC isozymes in cultured murine hair epithelial cells as well as PKC isozyme localization in murine dorsal skin at different stages in the hair cycle.Results  We observed that procyanidin B-2 reduces the expression of PKC-α, -βΙ, -βΙΙ and -η in cultured murine hair epithelial cells and also inhibits the translocation of these isozymes to the particulate fraction of hair epithelial cells. Our immunohistochemical analyses demonstrated that PKC-α, -βΙ, -βΙΙ and -η are specifically expressed in the outer root sheaths of both anagen and telogen hair follicles. The hair matrix at the anagen stage showed no positive staining for these PKC isozymes. Moderate to intense staining for PKC-βΙ and -βΙΙ in the epidermis and hair follicles was observed in a telogen-specific manner; however, expression of PKC-α and -η during the telogen stage was not conspicuous. Gö 6976, an inhibitor of calcium-dependent (conventional) PKC, proved to promote hair epithelial cell growth.Conclusions  These results suggest that PKC isozymes, especially PKC-βΙ and -βΙΙ, play an important role in hair cycle progression and that the hair-growing mechanisms of procyanidin B-2 are at least partially related to its downregulation of PKC isozymes or its inhibition of translocation of PKC isozymes to the particulate fraction of hair epithelial cells.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 76 (1994), S. 4761-4765 
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: Nb/Al-AlOx/Nb superconducting tunnel junctions for the application of radiation detectors were fabricated using dc magnetron sputtering. The surface morphology of polycrystalline Nb films was observed using an atomic force microscope. Junctions with various Al film thicknesses were fabricated. The leakage current of the junctions is found to decrease as the Al thickness increases, indicating an improvement of the Al film coverage over the Nb surface. X rays were detected with the junctions prepared and the low leakage property across the junction makes possible x-ray detection with various size junctions up to 200×200 μm2.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Woodbury, NY : American Institute of Physics (AIP)
    Applied Physics Letters 58 (1991), S. 881-883 
    ISSN: 1077-3118
    Source: AIP Digital Archive
    Topics: Physics
    Notes: Band-structure analysis using the tight-binding method indicates that there is a significant dependence of lasing properties of GaAs/AlGaAs quantum well lasers on substrate orientation, which suggests the importance of choosing the substrate orientation carefully for improving lasing properties. These results are mainly due to changes in the in-plane effective mass of the heavy hole.
    Type of Medium: Electronic Resource
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