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  • 1
    Electronic Resource
    Electronic Resource
    Decolorization of Brown Pigments in Foods by Immobilized Mycelia of Coriolus versicolor IFO 30340 and Paecilomyces canadensis NC-1 (2000)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 65 (2000), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Three microorganisms were cultured to investigate their decolorizing activity on mixtures of 2 types of model brown pigment and 2 types of browned food. The mixture of model brown pigments of the Maillard reaction type and phenol type was not decolorized by cultivation with Coriolus versicolor IFO 30340, but it was decolorized by Paecilomyces canadensis NC-1 and by Streptomyces werraensis TT 14. The immobilized mycelia of C. versicolor IFO 30340 and P. canadensis NC-1 were incubated simultaneously or successively with each mixture of 2 types of browned food. It was found that the mixed culture of these 2 mycelia showed a higher decolorization rate than their successive culture.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.2000.tb13602.x
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  • 2
    Electronic Resource
    Electronic Resource
    Changes in Free Amino Acids and Creatine Contents in Yellowtail (Seriola quinqueradiata) Muscle during Ice Storage (1982)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 47 (1982), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Free amino acids (FAA) and creatine contents of white and dark muscles of yellowtail (Seriola quinqueradiata) were measured and compared during ice storage. White muscle contained a large quantity of free histidine (1,160 mg/100g), while an equally large amount of taurine (1,150 mg/100g) was present in the dark muscle. Little change in the levels of most FAA occurred in the white muscle during ice storage for over 40 days but in the dark muscle the levels of almost all FAA except taurine increased significantly over a period of 33 days. Creatine was present in higher concentration in the white than in the dark muscle (510 vs 170 mg/100g), while its level did not change in either muscle during the entire storage period.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.1982.tb05006.x
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  • 3
    Electronic Resource
    Electronic Resource
    Studies on components 1' (modified monomer) and 2 (dimer) formed during heat-treatment of bovine serum albumin (1984)
    Springer
    Colloid & polymer science 262 (1984), S. 470-476 
    Details
    ISSN: 1435-1536
    Keywords: Bovine serum albumin ; Heat denaturation ; Isoelectric focusing ; Hydrogen ion titration curve ; Circular dichroism ; Fluorescence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract When the solution of bovine serum albumin at pH 9 is incubated at 65 °C, new components 1' (modified monomer), 2 (dimer) and 3 (probably trimer) are formed. The isoelectric focusing indicated that the isoelectric points of components 1', 2 and 3 were pH 5.9. The hydrogen ion titration curve for the native albumin had well known abnormal steepness at pH near 4, but that for component 1' had no abnormal steepness. The titration curve for component 1' located right side of that for the native albumin. Circular dichroism measurements indicated that some unfolding occurred, when the native albumin was changed to component 1'. The contents ofα-helix were 74 and 52 % for the native albumin and for component 1', respectively. The contents of β-structure were 19 and 23 % for the native albumin and for component 1', respectively. The wavelength of maximum intensity of tryptophan fluorescence shifted to lower wavelength, when the native albumin was changed to component 1'. This suggests that tryptophan residue(s) is transferred to a more hydrophobic environment. The hydrogen ion titration curves, circular dichroism and fluorescence measurements, all supported the possibility that the component 2 is formed by the dimerization of component 1'. Further, it has been found that component 2' (dimer impurity in commercial bovine serum albumin preparations) is formed by the direct dimerization of native bovine serum albumin without conformational change.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01412043
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  • 4
    Electronic Resource
    Electronic Resource
    Can Heart Rate Variability Predict Sudden Death? A Case of Sudden Death in a Child with Severe Coronary Sequelae of Kawasaki Disease (2000)
    Springer
    Pediatric cardiology 21 (2000), S. 403-406 
    Details
    ISSN: 1432-1971
    Keywords: Key words: Fractal scaling — Heart rate variability — Sudden death — Kawasaki disease
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. We report the heart rate variability (HRV) of a child aged 11 years. Arrhythmia was the suspected cause of sudden death after 10 years of therapy for Kawasaki disease. The linear methods failed to show any features of the HRV that could have predicted the patient's sudden death, but the fractal scaling as a nonlinear method had suddenly decreased from 5.3 to 4.1 1 year before his death.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002460010094
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  • 5
    Electronic Resource
    Electronic Resource
    The genome structure of kyuri green mottle mosaic tobamovirus and its comparison with that of cucumber green mottle mosaic tobamovirus (2000)
    Springer
    Archives of virology 145 (2000), S. 1067-1079 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary.  The genome of the Y strain of kyuri green mottle mosaic virus (KGMMV-Y) has been completely sequenced. Its genomic structure and sequence show it to be a typical tobamovirus, that is closest to, but distinct from, that of cucumber green mottle mosaic tobamovirus (CGMMV). The genomic sequence of KGMMV-Y was compared in detail with that of the SH strain of CGMMV. The sequences of their 5′- and 3′-untranslated regions were 74% and 63% identical. The amino acid sequences of the shorter and longer (read through) RNA replicase components, movement protein (MP) and coat protein (CP) were 58, 58. 60 and 46% identical, respectively. The KGMMV-Y genome sequence was also compared partly to that of another strain of KGMMV, KGMMV-C. The CP sequences of KGMMV-Y and KGMMV-C differed by 20 amino acid residues, suggesting that their relationship is more distant than the relationship between CGMMV-SH and CGMMV-W whose CP sequences are identical. The MPs of KGMMV-Y and KGMMV-C, however, differ only by one amino acid residue, although three amino acid substitutions are present in the MPs between CGMMV-SH and CGMMV-W. Two long stretches, one in the RNA replicase and the other in the MP, were highly conserved in KGMMV and CGMMV.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s007050070110
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  • 6
    Electronic Resource
    Electronic Resource
    Molecular characterization of the SCAR markers tightly linked to the Tm-2 locus of the genus Lycopersicon (2000)
    Springer
    Theoretical and applied genetics 101 (2000), S. 64-69 
    Details
    ISSN: 1432-2242
    Keywords: Key words Tomato ; Random amplified polymorphic DNA (RAPD) ; Sequence characterized amplified region (SCAR) ; Tm-2 gene ; Tomato mosaic virus (ToMV) resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The Tm-2 gene and its alleles conferring tomato mosaic virus resistance in tomato originate from Lycopersicon peruvianum, a wild relative of tomato. DNA fragments of several RAPD markers tightly linked with the Tm-2 locus in tomato were successfully cloned and sequenced. Subsequently, the 24-mer oligonucleotide primer pairs of the SCAR markers corresponding to the RAPD markers were designed based on the 5’-endmost sequences. A fragment of the same size as that of a SCAR marker was amplified in the ToMV-susceptible tomato line with no Tm-2, but the digests of the PCR fragments by AccI exhibited polymorphism in fragment length between the two lines. We chose three SCAR markers and three RAPD markers tightly linked with the Tm-2 locus, and examined whether the same-sized fragments corresponding to these markers were also present in three other lines carrying Tm-2a or one of the other Tm-2 alleles. The fragments corresponding to the three SCAR markers were present in all of the three lines, but the other markers (three RAPDs ) were absent in one or two lines, suggesting that the three SCAR markers are closer to Tm-2 than the other markers. Comparison of the nucleotide sequences of these fragments revealed that they are all homologous to the corresponding SCAR markers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051450
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  • 7
    Electronic Resource
    Electronic Resource
    Chromosome damage induced by artificial seed aging in barley (1984)
    Springer
    Theoretical and applied genetics 67 (1984), S. 161-170 
    Details
    ISSN: 1432-2242
    Keywords: Artificial seed aging ; Barley ; Mitotic and meiotic aberrations ; Pollen and seed fertilities ; Transmission of chromosomal aberrations
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Barley (Hordeum vulgare L. ‘Himalaya’) seeds were artificially aged under two storage conditions (32 °C/12% moisture content (m.c.) and 38 °C/18% m.c.) to study the behavior of induced chromosomal aberrations during plant growth. The frequencies of aberrant anaphases at first mitosis in root tips were correlated with loss of germinability. However, after 3 and 5 weeks' growth, aberration frequency declined. In plants grown from artificially aged seeds, the frequency of aberrant anaphases appeared to be stabilized at about 1% after 5 weeks' growth, in spite of the large differences in the frequencies at first mitosis. This suggests that because of their genetic imbalance, cells with chromosomal aberrations induced by seed aging were being excluded during plant growth. Meiotic chromosome configurations at MI were normal (7 II) in all plants studied, although a few precocious separations were found. Meiotic aberrations were found at AI-TI, AII-TII and the tetrad stages in the pollen mother cells of plants grown from the control and artificially aged seeds. However, there were no clear differences among the control and the two aging treatments. It was obvious that some cells with meiotic chromosomal aberrations were lost between the AI-TI and AII-TII stages, and still more between the AII-TII and tetrad stages. The frequency of tetrads with micronuclei in plants produced from artificially aged seeds was the same as in the control. The plants grown from artificially aged seeds showed high pollen fertility (95.2 to 97.0%) and seed fertility (90.1 to 97.2%) which was comparable to the control values (97.4 and 97.9%) respectively, indicating no special effects of seed aging. Anaphase cells of the first mitosis in the next (A2) generation were analyzed to study the transmission of chromosomal aberrations through mitotic and meiotic cell divisions in the A1 generation. Aberrant anaphases in the progeny from the artificially aged seeds were not higher than those of the control progeny. This indicates that the chromosomal aberrations induced by seed aging are not transmitted to the next generation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00317025
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