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  • Electronic Resource  (3)
  • 1995-1999  (3)
  • Achlorophyllous alga  (1)
  • Acidosomes  (1)
  • Keywords Type II diabetes mellitus  (1)
  • Calcium mobilization
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Material
  • Electronic Resource  (3)
Years
  • 1995-1999  (3)
Year
Keywords
  • 1
    Electronic Resource
    Electronic Resource
    Suppression of transforming growth factor beta and vascular endothelial growth factor in diabetic nephropathy in rats by a novel advanced glycation end product inhibitor, OPB-9195 (1999)
    Springer
    Diabetologia 42 (1999), S. 579-588 
    Details
    ISSN: 1432-0428
    Keywords: Keywords Type II diabetes mellitus ; extracellular matrix ; glomerulosclerosis ; type IV collagen ; Otsuka-Long-Evans-Tokushima-Fatty rat.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Aims/hypothesis. Advanced glycation end products (AGEs) participate in the pathogenesis of diabetic nephropathy. We reported earlier that OPB-9195, a synthetic thiazolidine derivative and novel inhibitor of advanced glycation, prevented progression of diabetic glomerulosclerosis by lowering serum concentrations of advanced glycation end products and reducing their deposition in the glomeruli. Here, we examined their contribution and that of growth factors, such as transforming growth factor-β (TGF-β) and vascular endothelial growth factor (VEGF), to the progression of diabetic nephropathy. We also investigated the expression of type IV collagen in the kidneys of Otsuka-Long-Evans-Tokushima-Fatty (OLETF) rats, a Type II (non-insulin-dependent) diabetes mellitus model, after treatment with OPB-9195. Methods. Using northern blots and immunohistochemical techniques, we determined the renal expression of TGF-β and type IV collagen mRNAs and proteins in OLETF rats. We also examined OPB-9195's effects on renal expression of VEGF mRNA and protein. Results. Concomitant increases in TGF-β and type IV collagen expression were observed at each point in time in OLETF rats not given OPB-9195. In contrast, OPB-9195 treatment greatly suppressed the renal expression of TGF-β, VEGF and type IV collagen mRNAs and proteins to that seen in non-diabetic rats. Conclusion/interpretation. Since OPB-9195, an AGE-inhibitor, prevented the progression of diabetic nephropathy by blocking type IV collagen production and suppressing overproduction of two growth factors, TGF-β and VEGF, in diabetic rats, this compound warrants further investigation. [Diabetologia (1999) 42: 579–588]
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001250051198
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Acidification of the young phagosomes ofParamecium is mediated by proton pumps derived from the acidosomes (1997)
    Springer
    Protoplasma 196 (1997), S. 12-20 
    Details
    ISSN: 1615-6102
    Keywords: Acidosomes ; Concanamycin B ; Phagosomes ; Proton pumps ; Paramecium ; Vacuolar ATPase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Although it is generally accepted that phagosome acidification is induced through the activity of a vacuolar proton pump (V-ATPase) present on the phagosome membrane, exactly how these pumps are delivered to the phagosomes is not well understood. To study this question inParamecium, it was necessary to first show that an authentic V-ATPase was present on their phagosomal membranes. Three antibodies raised against V-ATPases or their subunits were each found to label one or two large digestive vacuoles (DVs) inParamecium multimicronucleatum when immunofluorescence microscopy was used. Using horseradish peroxidase immunocytochemistry to increase sensitivity, about 10 DVs were shown to contain a V-ATPase. In high magnification images and cryoultramicrotomy these proton pumps were found to be located on the acidosomes, suggesting the vacuolar proton pumps on the DVs originate from the acidosomes. The authenticity of the V-ATPase was further confirmed by its sensitivity to cold temperature and to the V-ATPase specific inhibitor, concanamycin B, which at 10 nM doubled the t1/2 for vacuole acidification. Thus, we conclude that (1) acidosomes and some DVs ofParamecium have a bona-fide concanamycin B-sensitive and cold-sensitive V-ATPase, (2) the V-ATPase is delivered to the young DVs during acidosome fusion, and (3) the V-ATPase is involved in vacuole acidification. Finally, we have now determined thatParamecium has two immunologically related V-ATPases that are involved in two very different functions, (1) the acidification of phagosomes and (2) fluid segregation in the contractile vacuole complexes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01281054
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Effects of some factors on protoplast formation of a microalga, Prototheca zopfii (1997)
    Springer
    World journal of microbiology and biotechnology 13 (1997), S. 355-356 
    Details
    ISSN: 1573-0972
    Keywords: Achlorophyllous alga ; enzymatic degradation ; protoplast formation ; Prototheca.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The effects of experimental factors on protoplast formation of Prototheca zopfii Kru¨ger in 0.85 m NaCl using Macerozyme R-200 were studied based on a fractional factorial experimental design. The rate of protoplast formation was mainly affected by the incubation temperature and the age of algal cells. The optimal condition for the maximum protoplast yield was determined based on a response surface model. These were: mid-logarithmic phase cells and Macerozyme concentration of 4% at a temperature of 35°C.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018555628895
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    Paper (German National Licenses)
    Fulltext
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