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  • Electronic Resource  (4)
  • 1995-1999  (4)
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  • Electronic Resource  (4)
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  • 1
    Electronic Resource
    Electronic Resource
    A giant nucleopore protein that binds Ran/TC4 (1995)
    [s.l.] : Nature Publishing Group
    Nature 376 (1995), S. 184-188 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] RAN/TC4 is a small nuclear G protein1 that forms a complex with the chromatin-bound guanine nucleotide release factor RCC1 (ref. 2). Loss of RCC1 causes defects in cell cycle progression3,4, RNA export5-7 and nuclear protein ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/376184a0
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  • 2
    Electronic Resource
    Electronic Resource
    Availability of colloidal ferric oxides to coastal marine phytoplankton (1995)
    Springer
    Marine biology 122 (1995), S. 1-11 
    Details
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cell growth of a coastal marine diatom, Phaeodactylum tricornutum (stock cultures), and two red tide marine flagellates, Heterosigma akashiwo and Gymnodinium mikimotoi (stock cultures), in the presence of soluble chelated Fe(III)-EDTA (1:2) and of four different phases of ferric oxide colloids were experimentally measured in culture experiments at 20°C under 3000 lux fluorescent light. Soluble Fe(III)-EDTA induced the maximal growth rates and cell yields. The short-term uptake rate of iron by H. akashiwo in Fe(III)-EDTA medium was about eight times faster than that in solid amorphous hydrous ferric oxide (Fe2O3·xH2O) medium. In culture experiments supplied with four different ferric oxide forms, the orders of cell yields are amorphous hydrous ferric oxide〉γ-FeOOH (lepidocrocite)〉Fe5O7(OH)·4H2O (hydrated ferric oxyhydroxide polymer 〉α-FeOOH (goethite). The specific growth rates (μ) at logarithmic growth phase in Fe(III)-EDTA, amorphous hydrous ferric oxide and γ-FeOOH media were significantly greater than those in Fe5O7 (OH)·4H2O and α-FeOOH media. The thermodynamically stable forms such as Fe5O7(OH)·4H2O and α-FeOOH supported a little or no phytoplankton growth. The iron solublities and/or proton-promoted iron dissolution rates of these colloidal ferric oxides in seawater at 20°C were determined by simple filtration techniques involving γ-activity measurements of 59Fe. The orders of solubilities and estimated dissolution rate constants of these ferric oxides in seawater were consistent with that of cell yields in the culture experiments. These results suggest that the availability of colloidal iron to provide a source of iron for phytoplankton is related to the thermodynamic stability and kinetic lability of the colloidal ferric oxide phases, which probably control the uptake rate of iron by phytoplankton.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00349272
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Effect of natural and synthetic organic-Fe(III) complexes in an estuarine mixing model on iron uptake and growth of a coastal marine diatom, Chaetoceros sociale (1999)
    Springer
    Marine biology 134 (1999), S. 761-769 
    Details
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cell growth of a coastal marine diatom, Chaetoceros sociale, in the presence of different premixed organic-Fe(III) complexes [EDTA-Fe(III) (100:1 and 2:1), citric-Fe(III) (100:1) and fulvic-Fe(III) (0.1, 0.2 and 1 ppm C)] and solid amorphous hydrous ferric oxide [am-Fe(III) or Fe(III) hydroxide] were experimentally measured in culture experiments at 10 °C under 3000 lux fluorescent light. Fulvic-Fe(III) (0.1 and 0.2 ppm C) and citric-Fe(III) (100:1) induced maximal cell yields of C. sociale. The order of cell yields was: fulvic-Fe(III) (0.1 and 0.2 ppm C) ≥ citric-Fe(III) (100:1) 〉 EDTA-Fe(III) (2:1) ≫ solid am-Fe(III) 〉 EDTA-Fe(III) (100:1) ≫ fulvic-Fe(III) (1 ppm C). The short-term iron uptake rates by C. sociale in fulvic-Fe(III) (0.1 and 0.2 ppm C) and citric-Fe(III) (100:1) media were about five to six times faster than those in EDTA-Fe(III) (100:1) and solid am-Fe(III) media. The dissociative precipitation rates of premixed organic-Fe(III) complexes in seawater at 10 °C were determined by simple filtration (0.025 μm) involving γ-activity measurements of 59Fe. The order of estimated initial Fe(III) dissociative precipitation rates of these organic-Fe(III) complexes in seawater were nearly consistent with those of cell yields in the culture experiments and short-term iron uptake rates by C. sociale [except for fulvic-Fe(III) (1 ppm C) medium]. In fulvic-Fe(III) (0.1 and 0.2 ppm C), citric-Fe(III) (100:1) and EDTA-Fe(III) (2:1) media, the concentrations of dissolved organic-Fe(III) complexes in initial culture experiments are prone to supersaturate under the culture conditions. The supersaturated dissolved organic-Fe(III) complex in seawater supplies biologically available inorganic Fe(III) species in culture media through its dissociation at high pH and high levels of seawater cations. Therefore, the natural dissolved organic-Fe(III) complexes supplied by riverine input may play an important role in supplying bioavailable iron in estuarine mixing system and coastal waters.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002270050593
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  • 4
    Electronic Resource
    Electronic Resource
    Bioavailable iron species in seawater measured by macroalga (Laminaria japonica) uptake (1995)
    Springer
    Marine biology 123 (1995), S. 173-178 
    Details
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The bioavailability of iron in seawater filtered through a 0.025-μm filter was investigated using 59Fe-labeled iron uptake by the macroalga Laminaria japonica (Areschoug: Phaeophyta) (collected in the northern Japan Sea 1993) as an assay. About 80% of the iron in the 0.025-μm filtered coastal seawater was soluble and/or small colloidal organically bound iron, associated with natural organic ligands forming complexes with ferric ion. After decomposition of the organic matter by ultraviolet (UV) irradiation, 55% of the iron addition [or 0.6 nM, nearly the concentration of Fe(OH) 2 + in equilibrium with amorphous hydrous ferric oxide in seawater at pH 8.0] in the 0.025-μm filtered coastal seawater was taken up by the macroalga. Since the iron concentrations in the 0.025-μm filtered coastal seawater are 0.1 to 2.0 nM and only 0.6 nM of the iron is likely available to biota over 1 to 2 d, we suggest that only small amounts of bioavailable iron exist in coastal seawater not affected by inflow from land and that a significant fraction of dissolved (〈0.025 μ) iron occurs in forms, such as organic iron complexes, other than the simple hydroxo-complex species predicted by thermodynamic models.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00350337
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