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  • Electronic Resource  (2)
  • 1990-1994  (2)
  • Disk cathode  (1)
  • Endotoxin  (1)
  • 1
    ISSN: 1434-0879
    Keywords: Endotoxin ; Urinary tract infection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Endotoxin is a component of the outer membrane of gram-negative rods (GNR). Since GNR are responsible for the majority of urinary tract infection (UTI), we measured the concentration of endotoxin in urine using chromogenic endotoxin-specific assay and examined its diagnostic utility in patients with suspected UTI. In all 18 urine samples with an endotoxin concentration exceeding 350 pg/ml and 2 samples with 10–350 pg/ml of endotoxin concentration, GNR were detected at a count of 104 cfu/ml. Negative for endotoxin were 3 samples of culture positive for grampositive cocci (GPC), 2 samples containing various bacterial contaminants and all 37 samples with no growth on culture. Two urine samples collected 5 h after antibiotic dosage showed negative culture for GNR but a significant concentration of endotoxin. In an in vitro experiment, a residual concentration of antibiotic in urine inhibited bacterial growth, leading to a falsenegative culture. These results suggest that chromogenic endotoxin assay is a reliable method for diagnosing UTI caused by GNR and detecting false-negative culture of GNR.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Medical & biological engineering & computing 31 (1993), S. S37 
    ISSN: 1741-0444
    Keywords: Cold-cathode diode ; Disk cathode ; Flash X-rays ; Photon energy control ; Pulsed X-rays ; Tube current control
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract Fundamental studies of a repetitive flash X-ray generator using a diskcathode radiation tube are described. The high-voltage pulser employed a modified two-stage surge-Marx circuit. The two condensers in the pulser were charged from 40 to 60 kV, and the electric charges were discharged to the X-ray tube repetitively to generate flash X-rays. The total capacity during the main discharge was 425 pF, and the maximum output voltage from the pulser was about 1·9 times the charged voltage. The flash X-ray tube of the demountable-diode type and was composed of a rodshaped anode tip made of tungsten, a disk cathode made of graphite and a tube body made of polymethylmethacrylate. The peak tube voltage was primarily determined by the anode-cathode (A-C) space, and the peak tube current was less than 0·5 kA. Thus the maximum photon energy could be easily controlled by varying the A-C space, and the tube current roughly increased according to increases in the charged voltage. The pulse width ranged from 40 to 100 ns, and the X-ray intensity was less than 1·1 μC kg−1 at 0·5 m per pulse. The repetition rate was less than 50 Hz, and the effective focal spot size was equivalent to the anode diameter.
    Type of Medium: Electronic Resource
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