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  • Electronic Resource  (5)
  • 1990-1994  (5)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Mycopathologia 118 (1992), S. 147-152 
    ISSN: 1573-0832
    Keywords: Fungi ; cystine ; sulfitolysis ; sulfate ; thiosulfate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Twenty-six different species of keratinophilic fungi were examined to determine their ability to utilize free cystine. Of the fungi tested, the majority metabolized free L-cystine in a glucose-peptone culture medium. Cystine was used as source of sulfur, and carbon and nitrogen as well. Excess sulfur was excreted into the culture fluid, as thiosulfate and sulfate, following oxidation. The rate of cystine oxidation varied with the different fungal strains, but was maximal for Graphium penicilloideus (88.5%). Low quantities of thiols were found in the medium. Cystine oxidation and inorganic thiosulfate excretion were found to correlate significantly (r = 0.94).
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-0832
    Keywords: Fungi ; keratin degradation ; sulfhydryl compounds ; sulfate ; thiosulfate ; keratinase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The ability of five keratinophilic fungi, i.e., Chrysosporium indicum, Geotrichum candidum, Gymnoascoideus petalosporus, Scopulariopsis brevicaulis, and Talaromyces trachyspermus, to digest human hair keratin in stationary culture has been studied. Degradation of human scalp hair was studied by determination of cysteine, cystine, inorganic sulfate, thiosulfate, total protein, keratinase and change in alkalinity of culture filtrate. Gymnoascoideus petalosporus showed maximum degradation as compared to remaining isolates when grown on human scalp hair as the sole source of nutrients ‘in vitro’.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1573-0832
    Keywords: Fungi ; keratinase ; regulation ; synthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Chrysosporium queenslandicum, Graphium penicilloideus andScopulariopsis brevicaulis were grown on various supplemented basal salts media to compare keratinase induction, activity and repression. All three fungi can utilize keratin as a sole source of carbon, nitrogen and sulfur. Total keratinase activity inC. queenslandicum andS. brevicaulis, was not repressed by supplementation of keratin-containing medium with glucose, ammonium or sulfate. The production of keratinase activity was not derepressible in keratin-free media. Keratin utilization commenced before the detection of significant extracellular keratinase activity which was always associated with mycelial growth.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1573-0832
    Keywords: Keratinolysis ; fungi ; inorganic sulphate ; thiosulphate ; keratinase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Absidia cylindrospora and Rhizomucor pusillus, causal agents of phycomycoses, were cultured on sterile natural keratins in a mineral solution and the keratin degradation products analyzed. The excess of sulphur was removed by oxidation to inorganic sulphate and thiosulphate, which were the main products of sulphitolysis of keratin. The proteolytic activity of the two fungi depended on the nature of the keratin substrate. Human scalp hair was the most favoured keratin substrate by both the fungi.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1573-0832
    Keywords: S. brevicaulis ; keratinase purification ; partial characterization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Two extracellular keratinases of Scopulariopsis brevicaulis were purified and partially characterized. The enzymes were isolated by the techniques of gel filtration chromatography and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). These keratinases (K I & K II) were purified approximately 33 and 29 fold, respectively. SDS-PAGE of the products of gel filtration chromatography (K I & II) produced only one band each, suggesting homogeneity. The optimum pH for both keratinases was 7.8, while the optimum temperatures were 40°C (K I) and 35°C (K II). Estimated molecular weights were 40–45 KDa and 24–29 KDa for K I & K II respectively. Both keratinases were inhibited by phenylmethylsulfonyl fluoride which suggests a serine residue at or near an active site.
    Type of Medium: Electronic Resource
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