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  • Digitale Medien  (13)
  • Chemistry  (9)
  • (Rb. sphaeroides)  (2)
  • Dominant markers  (2)
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Materialart
  • Digitale Medien  (13)
Erscheinungszeitraum
Schlagwörter
  • 1
    Digitale Medien
    Digitale Medien
    Is bicarbonate in Photosystem II the equivalent of the glutamate ligand to the iron atom in bacterial reaction centers?
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular 1100 (1992), S. 1-8 
    Details
    ISSN: 0167-4838
    Schlagwort(e): (Rb. sphaeroides) ; Bicarbonate effect ; Electron acceptor complex ; Electron transfer ; Photosynthesis ; Quinone ; Reaction center ; Site-directed mutagenesis
    Quelle: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Thema: Biologie , Chemie und Pharmazie , Medizin
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1016/0167-4838(92)90529-M
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  • 2
    Digitale Medien
    Digitale Medien
    Is bicarbonate in Photosystem II the equivalent of the glutamate ligand to the iron atom in bacterial reaction centers?
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/Bioenergetics 1100 (1992), S. 1-8 
    Details
    ISSN: 0005-2728
    Schlagwort(e): (Rb. sphaeroides) ; Bicarbonate effect ; Electron acceptor complex ; Electron transfer ; Photosynthesis ; Quinone ; Reaction center ; Site-directed mutagenesis
    Quelle: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Thema: Biologie , Chemie und Pharmazie , Medizin , Physik
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1016/0005-2728(92)90119-M
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  • 3
    Digitale Medien
    Digitale Medien
    TransgenicArabidopsis tester lines with dominant marker genes (1996)
    Springer
    Molecular genetics and genomics 251 (1996), S. 365-372 
    Details
    ISSN: 1617-4623
    Schlagwort(e): Arabidopsis thaliana ; Dominant markers ; Map-based cloning ; Recombinant selection ; T-DNA localization
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The map positions of a set of eight T-DNA insertions in theArabidopsis genome have been determined by using closely linked visible markers. The insertions are dispersed over four of the five chromosomes. Each T-DNA insert contains one or more of the chimeric marker genes neomycin phosphotransferase (neo), hygromycin phosphotransferase (hpt), phosphinothricin acetyltransferase (bar),β-glucuronidase (gusA) and indole-3-acetamide hydrolase (iaaH). Theneo, hpt andbar marker genes are dominant in a selective germination assay or when used as DNA markers in a polymerase chain reaction. These dominant markers will allow recombinants to be discerned in a germinating F2 population, one generation earlier than with a conventional recessive marker. The transgenic marker lines will speed up and simplify the isolation of recombinants in small genetic intervals, a rate-limiting step in positional cloning strategies. The transgenic lines containing thehpt marker will also be of interest for the isolation of deletion mutants at the T-DNA integration sites.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02172528
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  • 4
    Digitale Medien
    Digitale Medien
    Transgenic Arabidopsis tester lines with dominant marker genes (1996)
    Springer
    Molecular genetics and genomics 251 (1996), S. 365-372 
    Details
    ISSN: 1617-4623
    Schlagwort(e): Key words Arabidopsis thaliana ; Dominant markers ; Map-based cloning ; Recombinant selection ; T-DNA localization
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  The map positions of a set of eight T-DNA insertions in the Arabidopsis genome have been determined by using closely linked visible markers. The insertions are dispersed over four of the five chromosomes. Each T-DNA insert contains one or more of the chimeric marker genes neomycin phosphotransferase (neo), hygromycin phosphotransferase (hpt), phosphinothricin acetyltransferase (bar), β-glucuronidase (gusA) and indole-3-acetamide hydrolase (iaaH). The neo, hpt and bar marker genes are dominant in a selective germination assay or when used as DNA markers in a polymerase chain reaction. These dominant markers will allow recombinants to be discerned in a germinating F2 population, one generation earlier than with a conventional recessive marker. The transgenic marker lines will speed up and simplify the isolation of recombinants in small genetic intervals, a rate-limiting step in positional cloning strategies. The transgenic lines containing the hpt marker will also be of interest for the isolation of deletion mutants at the T-DNA integration sites.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02172528
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  • 5
    Digitale Medien
    Digitale Medien
    Extrudate roughness of high-density polyethylene and linear low-density polyethylene: Determination of key parameters using screening design (1994)
    Stamford, Conn. [u.a.] : Wiley-Blackwell
    Polymer Engineering and Science 34 (1994), S. 1047-1055 
    Details
    ISSN: 0032-3888
    Schlagwort(e): Chemistry ; Chemical Engineering
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie , Maschinenbau , Physik
    Notizen: A screening design is used to establish the contribution of various parameters to the roughness of cylindrical extrudates. A dimensionless response variable is proposed to characterize the extrudate roughness, and the effects of ten parameters on this roughness response were examined. Two polyethylenes (one high density and one linear low density) were investigated using a 45 mm single screw extruder. The results show that the main parameters affecting the extrudate roughness are, in order of importance, apparent shear stress at the die wall, die diameter, ratio of die length to diameter, and type of polymer. The other six parameters (the use of an additive, recycling, type of entrance adapter, die material, die temperature, melt temperature) were found to have a non-significant contribution to roughness.
    Zusätzliches Material: 9 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/pen.760341303
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  • 6
    Digitale Medien
    Digitale Medien
    On the gel effect in the presence of a chain transfer agent in methyl methacrylate polymerization and its copolymerization with various acrylates (1993)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 49 (1993), S. 2179-2188 
    Details
    ISSN: 0021-8995
    Schlagwort(e): Chemistry ; Polymer and Materials Science
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie , Maschinenbau , Physik
    Notizen: The kinetics of methyl methacrylate (MMA) polymerization, and of its copolymerization with various acrylates, at high conversions in the presence of a chain transfer agent, are investigated with a dilatometer over the entire course of reaction. The displacement to higher conversions of the onset of the gel effect in the MMA homopolymerization, in the presence of a chain transfer agent, was determined. Similar information is also provided for the MMA-acrylate copolymerization systems. An increase in polymerization temperature slightly delays the onset of the gel effect in the MMA-acrylate copolymerization, but considerably increases the final conversion. The final conversion in copolymerization for a constant concentration of the chain transfer agent is independent of the initiator concentration, but is a function of the polymerization temperature. The reaction time for reaching the limiting conversion in copolymerization is increased with an increasing amount of the second monomer, as well as with an increasing number of carbon atoms in the acrylate used as the second monomer. © 1993 John Wiley & Sons, Inc.
    Zusätzliches Material: 9 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/app.1993.070491214
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  • 7
    Digitale Medien
    Digitale Medien
    Convective wall-to-suspension heat transfer in circulating fluidized bed risers (1996)
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 42 (1996), S. 2112-2117 
    Details
    ISSN: 0001-1541
    Schlagwort(e): Chemistry ; Chemical Engineering
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: A mechanistic model, which incorporates recent findings on the fluid dynamics in the riser of the circulating fluidized bed (CFB), is developed for predicting the suspension-to-wall heat-transfer coefficient in the riser. It is assumed that heat transfer between the gas-particle suspension and the riser wall takes place by the contact of both particle packets and an emulsion phase on the wall. A characteristic length (L), that is, a sliding distance of the emulsion phase along the heat-transfer surface, is introduced in the model, enabling the effect of the length of heat-transfer surface to be evaluated. It is found that the heat-transfer coefficient decreases with increasing L, but becomes increasingly insensitive to L when L is larger than 1 m. Agreement between model prediction and measurement is encouraging over a range of operating conditions, heat-transfer surface length, and riser diameters.
    Zusätzliches Material: 6 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/aic.690420803
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  • 8
    Digitale Medien
    Digitale Medien
    Lipase immobilized on hydrophobic porous polymer supports prepared by concentrated emulsion polymerization and their activity in the hydrolysis of triacylglycerides (1993)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 42 (1993), S. 821-828 
    Details
    ISSN: 0006-3592
    Schlagwort(e): lipase ; polymer supports ; emulsion polymerization ; immobilized enzyme ; hydrolysis ; triacylglycerides ; Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Microporous polymer supports for the immobilization of lipase have been prepared by the polymerization of a concentrated emulsion precursor. The concentrated emulsion consists of a mixture of styrene and divinyl-benzene containing a suitable surfactant and an initiator as the continuous phase and water as the dispersed phase. The volume fraction of the latter phase was greater than 0.74, which is the volume fraction of the dispersed phase for the most compact arrangement of spheres of equal radius. The lipase from Candida rugosa has been immobilized on the internal surface of the hydrophobic microporous poly(styrene-divinyl benzene) supports and used as biocatalysts for the hydrolysis of triacylglycerides. The effects of the amount of surfactant, of the molar ratio of divinylbenzene/styrene in the continuous phase, and of the aquaphilicity of the supports on the adsorption, activity, and stability of the immobilized lipase have been investigated. The microporous poly(styrene-divinylbenzene) adsorbents constitute excellent supports for lipase because both the amount adsorbed is large and the rate of enzymatic reaction per molecule of lipase is higher for the immobilized enzyme than for the free one. © 1993 John Wiley & Sons, Inc.
    Zusätzliches Material: 8 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/bit.260420706
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  • 9
    Digitale Medien
    Digitale Medien
    Porous-coated titanium implant impregnated with a biodegradable protein delivery system (1997)
    Hoboken, NJ : Wiley-Blackwell
    Journal of Biomedical Materials Research 36 (1997), S. 516-521 
    Details
    ISSN: 0021-9304
    Schlagwort(e): porous-coated ; titanium ; PLA-PGA ; protein release ; degradation ; Chemistry ; Polymer and Materials Science
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Medizin , Technik allgemein
    Notizen: Tissue ingrowth into porous-coated orthopedic and dental implants is commonly used as a means to achieve long-term fixation of these prostheses. However, the degree of tissue ingrowth is often inadequate and inconsistent. If the pores of these implants are impregnated with a controlled drug release system delivering relevant growth factors, then it might be possible to stimulate more tissue ingrowth. The present study introduces such a system based on biodegradable polymers and investigates its protein release profile and polymer degradation characteristics. Porous coated titanium implants were impregnated with a mixture of a 50%-50% polylactic acid-polyglycolic acid copolymer and a model protein, soybean trypsin inhibitor. Control implants contained only the polymer and no protein. The implants were subjected to hydrolytic degradation in phosphate buffered saline at 37°C for periods of 3, 6, and 11 weeks. The protein release and the mass and molecular weight of the polymer were monitored. The results indicate that the protein is released in three distinct phases and the polymer loses almost all its mass and molecular weight by 11 weeks. There was a significant difference in the polymer degradation characteristics between the control and test implants, which might be the result of some complex polymer-protein interactions. © 1997 John Wiley & Sons, Inc. J Biomed Mater Res, 36, 516-521, 1997.
    Zusätzliches Material: 5 Ill.
    Materialart: Digitale Medien
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  • 10
    Digitale Medien
    Digitale Medien
    XPS analysis of hydroxide ion surface reactions on reactions on CeF3 and LaF3 fluoride ion-selective electrodes (1997)
    Weinheim : Wiley-Blackwell
    Electroanalysis 9 (1997), S. 917-921 
    Details
    ISSN: 1040-0397
    Schlagwort(e): XPS analysis ; Fluoride ; Ion selective electrode ; Surface analysis ; Hydroxide interference ; Chemistry ; Polymer and Materials Science
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie
    Notizen: Lanthanum fluoride and cerium fluoride single crystals, used as the sensing membranes of the fluoride ion-selective electrode (F-ISE), were investigated for their hydroxide interference and surface reactions with OH- in high pH conditions. While these membranes show fast response and excellent Nernstian behavior over a wide concertration range in buffered F- solutions, they deviate from the theoretical slope at high pH. CeF3, in particular, exhibits a much larger deviation from the Nernstian slope and a substantially slower response to a F- activity change compared to LaF3. This larger deviation is due to more extensive and faster formation of hydroxo-complexes, with the release of a greater amount of the fluoride ion into the hydrated gel layer. The slower response of the CeF3 membrane after contact with OH- is due to the formation of CeIV oxide on the surface, which acts as a blocking layer to the exchange of F-.
    Zusätzliches Material: 4 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/elan.1140091210
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