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  • Electronic Resource  (7)
  • Human tissue  (2)
  • Nicotiana  (2)
  • Tobacco BY-2 cells  (2)
  • 1-decanethiol  (1)
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  • Electronic Resource  (7)
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Keywords
  • 1
    Electronic Resource
    Electronic Resource
    A Rydberg-atom ionization source for negative ion mass spectrometry
    Amsterdam : Elsevier
    International Journal of Mass Spectrometry and Ion Processes 123 (1993), S. 217-223 
    Details
    ISSN: 0168-1176
    Keywords: 1-decanethiol ; 3-nitrobenzyl-alcohol ; Rydberg-atom ionization ; negative ion mass spectrometry ; o-nitrophenyl octyl ether.
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0168-1176(93)87100-7
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  • 2
    Electronic Resource
    Electronic Resource
    Enrichment of heterokaryocytes between mesophyll and epidermis protoplasts by density gradient centrifugation after electric fusion (1987)
    Springer
    Theoretical and applied genetics 75 (1987), S. 26-29 
    Details
    ISSN: 1432-2242
    Keywords: Density gradient centrifugation ; Electric fusion ; Epidermis protoplasts ; Heterokaryocyte enrichment ; Mesophyll protoplasts ; Nicotiana
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Mesophyll protoplasts from Nicotiana glauca were fused with epidermal protoplasts from N. langsdorffii by an electric pulse. After the fusion products were centrifuged on stepwise density gradient centrifugation using Percoll and sea water, somatic hybrids were observed at 70%–80% in the fraction recovered from the intermediate specific gravity fraction between epidermis and mesophyll protoplasts. From offsprings of these somatic hybrids, teratomatous plants were regenerated. Since the difference of specific gravity between mesophyll and epidermis protoplasts is inherent, this procedure can be essentially applied to obtain somatic hybrids between any combination of plants. The significance of this study is discussed in relation to obtaining somatic hybrids between plant materials without any appropriate genetic markers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00249137
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  • 3
    Electronic Resource
    Electronic Resource
    Microtubule organizing centers in plant cells: localization of a 49 kDa protein that is immunologically cross-reactive to a 51 kDa protein from sea urchin centrosomes in synchronized tobacco BY-2 cells (1993)
    Springer
    Protoplasma 176 (1993), S. 64-74 
    Details
    ISSN: 1615-6102
    Keywords: Cell cycle ; Microtubule ; Microtubule organizing center ; Synchronization ; Tobacco BY-2 cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A 49 kDa protein in tobacco BY-2 cells has been found to be cross-reactive with antibodies raised against a 51 kDa protein that was isolated from sea urchin centrosomes and identified as a microtubule-organizing center (MTOC) in animal cells. Tracing the fate of the 49 kDa protein during progression of the cell cycle in highly synchronized tobacco BY-2 cells revealed that this protein was colocalized with plant microtubules (MTs): the location of the 49 kDa protein coincided with preprophase bands (PPBs), mitotic spindles and phragmoplasts. Furthermore, between the M and G1 phases, the 49 kDa protein was observed in the perinuclear regions, in which the initials of MTs are organizing to form cortical MTs. At the G1 phase the location of the 49 kDa protein in the cell cortex coincided with that of the cortical MTs. It appeared that the 49 kDa protein in the cell cortex was transported as granules from the perinuclear regions. Thus, it is highly probable that the 49 kDa protein, which reacts with antibodies against the 51 kDa protein in sea urchin centrosomes, plays the role of an MTOC in plant cells. Thus, the mechanisms for organizing MTs in higher organisms appear to share a common protein, even though the organization of MTs is superficially very different in plant and animal cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01378940
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  • 4
    Electronic Resource
    Electronic Resource
    Organization of heterogeneous mitochondrial DNA molecules in mitochondrial nuclei of cultured tobacco cells (1993)
    Springer
    Protoplasma 175 (1993), S. 112-120 
    Details
    ISSN: 1615-6102
    Keywords: Cell culture ; DNA content ; Mitochondrial DNA ; Mitochondrial genome ; Nicotiana
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The molecular size of mitochondrial DNA (mtDNA) molecules and the number of copies of mtDNA per mitochondrion were evaluated from cultured cells of the tobacco BY-2 line derived fromNicotiana tabacum L. cv. Bright Yellow-2. To determine the DNA content per mitochondrion, protoplasts of cultured cells were stained with 4′,6-diamidino-2-phenylindole (DAPI), and the intensity of the fluorescence emitted from the mitochondrial nuclei (mt-nuclei) was measured with a video-intensified photon counting microscope system (VIM system). Each mitochondrion except for those undergoing a division contained one mt-nucleus. The most frequently measured size of the DNA in the mitochondria was between 120 and 200 kilobase pairs (kbp) throughout the course of culture of the tobacco cells. Mitochondria containing more than 200 kbp of DNA increased significantly in number 24 h after transfer of the cells into fresh medium but their number fell as the culture continued. Because division of mitochondria began soon after transfer of the cells into fresh medium and continued for 3 days, the change of the DNA content per mitochondrion during the culture must correspond to DNA synthesis of mitochondria in the course of mitochondrial division. By contrast, the analyses of products of digestion by restriction endonucleases indicated that the genome size of the mtDNA was at least 270 kbp. Electron microscopy revealed that mtDNAs were circular molecules and their length ranged from 1 to 35 μm, and 60% of them ranged from 7 to 11 μrn. These results indicate that the mitochondrial genome in tobacco cells consists of multiple species of mtDNA molecules, and mitochondria do not contain all the mtDNA species. Therefore, mitochondria are heterogeneous in mtDNA composition.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01385008
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  • 5
    Electronic Resource
    Electronic Resource
    Sites of microtubule reorganization in tobacco BY-2 cells during cell-cycle progression (1997)
    Springer
    Protoplasma 198 (1997), S. 202-209 
    Details
    ISSN: 1615-6102
    Keywords: Cell cycle ; Elongation factor 1α ; Microtubule reorganization ; Microfilament ; Tobacco BY-2 cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The sites of microtubule (MT) reorganization were examined in synchronized tobacco BY-2 cells. The MTs of these cells were completely destroyed by a combined cold and drug treatment at 0 °C with 100 μM propyzamide for 3 h. After the cells were washed and cultured at 30 °C, the reorganization of MTs was observed in detail. Sites for MT reorganization at each stage of the cell cycle were identified on the cell cortex and nuclei, the mitotic apparatus, the nuclei (or the nuclei and cell cortex), and the cell cortex in the S-G2 phase, M phase, M/G1 interface, and g1 phase, respectively. The polypeptide synthesis elongation factor (EF)-1α is co-localized with these sites of MT reorganization. At some stages, microfilaments (MFs) were found to be involved in the reorganization of MTs. Based on these results, the mode of MT reorganization during cell cycle progression is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01287569
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  • 6
    Electronic Resource
    Electronic Resource
    A case of drowning linked to ingested sulfides — a report with animal experiments (1996)
    Springer
    International journal of legal medicine 109 (1996), S. 42-44 
    Details
    ISSN: 1437-1596
    Keywords: Toxicology ; Drowning ; Sulfides ; Human tissue ; Distribution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Law
    Notes: Abstract An adult male was found dead beneath a pool of sewage in the pump room of a fish market. Autopsy revealed the cause of death to be suffocation after aspirating sewage into the respiratory tract. Since hydrogen sulfide gas was detected in the atmosphere at the scene of the accident, gas poisoning was suspected and toxicological analysis of sulfides in body tissues was performed. The concentrations of sulfides in the blood, lung and kidney were 0.95 μmol/ml, 0.22 and 0.38 μmol/g, respectively. These values were remarkably higher than those in previously reported cases involving exposure to hydrogen sulfide gas. Therefore, oral intake of sulfides was assumed and the distribution of sulfides in tissues following oral administration of sodium sulfide solution was examined by means of animal experiments using rats. The concentration of sulfides in the blood from rats following oral intake was much higher than that seen following gas exposure. Based on these results, we concluded that the victim had been exposed to hydrogen sulfide gas and had then collapse into a pool of sewage containing sulfides. The sulfides which were distributed throughout the body tissues had mainly issued from the alimentary tract prior to death by drowning.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01369601
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  • 7
    Electronic Resource
    Electronic Resource
    Forensic analysis of triazolam in human tissues using capillary gas chromatography (1991)
    Springer
    International journal of legal medicine 104 (1991), S. 67-69 
    Details
    ISSN: 1437-1596
    Keywords: Triazolam ; Estazolam ; Capillary GC/NPD ; Human tissue ; Forensic toxicology ; Triazolam ; Estazolam ; Kapillar-Gaschromatographie/NPD ; Menschliches Gewebe ; Forensische Toxikologie
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Law
    Description / Table of Contents: Zusammenfassung Eine zuverlässige und empfindliche Methode wurde entwickelt, um die Konzentrationen des hypnotischen Medikaments Triazolam in menschlichen Geweben, einschließlich fauler Gewebe bestimmen zu können. Die Methode besteht aus einer dreistufigen Flüssig-Flüssig-Extraktion, einem Clean up an Kieselgel und Gaschromatographie mit einem Stickstoff-PhosphorDetektor und einer Kapillarsäule. Als interner Standard wurde Estazolam benutzt. Die Eichkurve war im Konzentrationsbereich zwischen 1 ng/g und 1 gg/g linear, und die untere Nachweisgrenze betrug 0.5 ng/g. Es wurde eine forensische Untersuchung über die toxikologischen Effekte von Triazolam an faulem Gewebe durchgeführt.
    Notes: Summary A reliable and sensitive method has been developed to assess the concentrations of the hypnotic drug triazolam in human tissues, including putrefied tissues. The method involves a 3-step solvent extraction, cleanup on a silica gel column and gas chromatography using a nitrogen phosphorus detector and a capillary column. Estazolam was used as an internal standard. The calibration curve was linear over the concentration range 1 ng/g1 gm/g and the lower limit of detection was 0.5 ng/g. A forensic study was performed on the toxicological effects of triazolam using putrefied tissues.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01626033
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