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  • Electronic Resource  (5)
  • Hyperresistance  (2)
  • MNNG  (2)
  • 3-carbethoxypsoralen  (1)
  • 4-NQO  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Hyperresistance to DNA damaging agents in yeast (1986)
    Springer
    Current genetics 11 (1986), S. 211-215 
    Details
    ISSN: 1432-0983
    Keywords: Saccharomyces cerevisiae ; Hyperresistance ; DNA damaging agents ; Genotoxic effects
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In order to study resistance to DNA damaging agents, yeast DNA segments conferring hyperresistance in this organism to such genotoxic agents were selected for among yeast cells transformed by a yeast genome library based on the multi-copy vector plasmid YEp13. Genetic variants hyperresistant to 4-nitroquinohne-N-oxide, formaldehyde, and alkylating agents were isolated and the respective hyperresistance determinants shown to co-segregate with the vector plasmid. Phenotypical characterization indicated different degrees of resistance, few cases of cross-resistance and differing structural stability of the cloned DNA. By transfer to E. coli and subsequent retransformation of yeast a number of plasmids was shown to stably carry the genetic information for hyperresistance.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00420609
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Hyperresistance to formaldehyde of Saccharomyces cerevisiae seems not to be correlated with the formation and removal of DNA protein cross-links (1988)
    Springer
    Current genetics 13 (1988), S. 125-128 
    Details
    ISSN: 1432-0983
    Keywords: Formaldehyde ; DNA-protein cross-links ; Repair ; Saccharomyces cerevisiae ; Hyperresistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The formation and removal of formaldehyde-mediated DNA protein cross-linking was measured by CsCI density gradient analysis in yeast strains of differing resistance to formaldehyde. Wild-type cells and transformants made hyperresistant to formaldehyde by a multi-copy vector containing the yeast SFA gene were specifically labeled in their DNA and incubated in the presence of formaldehyde. Treatment with formaldehyde lead to the formation of equal amounts of DNA protein cross-links; subsequent liquid holding of cells for 24 h resulted in the removal of nearly all DNA protein crosslinks regardless of the original formaldehyde resistance status of the strains.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00365646
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    The DNA repair gene PSO3 of Saccharomyces cerevisiae belongs to the RAD3 epistasis group (1992)
    Springer
    Current genetics 21 (1992), S. 85-90 
    Details
    ISSN: 1432-0983
    Keywords: pso and rad mutants ; Repair ; S. cerevisiae ; 8-methoxypsoralen ; 3-carbethoxypsoralen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The mutant allele pso3-1 of Saccharomyces cerevisiae confers sensitivity to treatment with UV365nm (UVA) light-activated mono- and bi-functional psoralens. When pso3-1 is combined in double mutants with selected rad and pso mutant alleles and subjected to 8-MOP+UVA treatment, epistatic interaction with regard to survival is observed with pso1, pso2, and rad3. With the same treatment the combination of pso3-1 with rad6 and rad52 leads to synergistic interaction. For the monofunctional agent 3-carbethoxypsoralen (3-CPs) the analysis of double mutants yields the same results as with the bifunctional 8-methoxypsoralen (8-MOP) with the exception of the pso1-1pso3-1 double mutant. Here we find an additive interaction, i.e., the sensitivities of both parental strains are summed in the double mutant, which indicates a different substrate specificity of the repair activity encoded by the PSO1 and PSO3 genes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00318660
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Isolation and characterization of additional genes influencing resistance to various mutagens in the yeast Saccharomyces cerevisiae (1992)
    Springer
    Current genetics 21 (1992), S. 319-324 
    Details
    ISSN: 1432-0983
    Keywords: Multi-copy plasmid ; Hyper-resistance ; 4-NQO ; MNNG ; Triaziquone
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Screening of a multi-copy vector-based yeast genomic library in haploid cells of wild-type Saccharomyces cerevisiae yielded transformants hyper-resistant to various chemical mutagens. Genetical analysis of the yeast insert DNAs revealed three genes SNG1, SNQ2, and SNQ3 that confer the phenotype hyper-resistance to MNNG, to 4-NQO and triaziquone, and to mutagens 4-NQO, MNNG, and triaziquone, respectively. Integration of the gene disruption-constructs into the haploid yeast genome yielded viable null-mutants with a mutagen-sensitive phenotype. Thus, copy number of these non-essential yeast genes determines the relative resistance to certain chemical mutagens, with zero copies yielding a phenotype of mutagen sensitivity and multiple copies one of mutagen hyper-resistance, respectively.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00351689
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Overexpression of the SNQ3/YAP1 gene confers hyper-resistance to nitrosoguanidine in Saccharomyces cerevisiae via a glutathione-independent mechanism (1994)
    Springer
    Current genetics 25 (1994), S. 469-471 
    Details
    ISSN: 1432-0983
    Keywords: Yeast ; GSH ; DNA alkylation ; MNNG
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The MNNG hyper-resistance of yeast transformants containing multiple copies of the SNQ3/YAP1 yeast gene is not caused by lowered MNNG activation due to depleted pools of glutathione. On the contrary, the SNQ3/YAP1-encoded protein stimulates production of GSH, apparently by promoter activation due to the AP-1 recognition element. Expression of at least one further gene, encoding a protein with a strong detoxifying activity, must also be stimulated to explain the MNNG hyper-resistance phenotype.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00351788
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    Paper (German National Licenses)
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