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  • Electronic Resource  (3)
  • Freeze-etching  (2)
  • Collagen fibrils  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Acta neuropathologica 45 (1979), S. 221-229 
    ISSN: 1432-0533
    Keywords: Mouse mutant Jimpy ; Optic nerve ; Lipid ultrastructure ; Phagolysosomes ; Phagocytes ; Histochemistry ; Freeze-etching ; Thin sections
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Optic nerves of the mouse mutant Jimpy and controls were studied in different developmental stages. The results cast some light on the nature and ultrastructure of intracellular lipids, which are a striking feature in the CNS of these affected mice. Light Microscopy. In the premyelination period the activity of acid phosphatase is quite similar in the lysosomes of both animal groups. A much higher increase of reaction product was found in Jimpy compared to controls during the advanced myelination stages. The normal glial cell arrangement in rows is absent in leucodystrophic animals. Electron Microscopy. Replicas of freeze-etched Jimpy optic nerves show lipid loaded cells exhibiting a plasma membrane different from that of astrocytes and oligodendrocytes, thus suggesting a relationship to phagocyting microglia. Furthermore, the rare myelin membranes are almost particle free. Three types of structurally different lipid inclusions could be observed. Most of the lipidic bodies are surrounded by a membrane studded with particles. The freeze-fractured morphology of the lipid inclusions was compared with the appearance in thin sections, incubated for acid phosphatase demonstration. Acid phosphatase reaction product within all three types of lipid containing vesicles indicate the lysosomal nature of these structures, which may represent myelin breakdown products and/or myelin precursors.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 146 (1973), S. 473-489 
    ISSN: 1432-0878
    Keywords: Pecten oculi ; Chicken ; Capillaries ; Transmission and scanning electron microscopy ; Freeze-etching
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Der Pecten oculi des Haushuhns wurde licht-, scanning- und transmissions-elektronenmikroskopisch sowie mit der Gefrierätz-Technik unter besonderer Berücksichtigung seiner Kapillarstruktur untersucht. Die Kapillaren bilden ausgedehnte anastomosierende Netze. Ihre Endothelzellen besitzen sowohl apikal (luminal) als auch basal längsorientierte Mikrofalten. Es wird angenommen, daß die Ausbildung der apikalen endothelialen Oberflächendifferenzierung haemodynamisch bedingt ist. Hierbei wird gleichzeitig ausreichend Platz für membrangebundene Fermente geschaffen, die für aktive energie-verbrauchende transzelluläre Transportprozesse benötigt werden. Die Gefrierätzbefunde lassen unterschiedlich strukturierte Membranoberflächen an den Mikrofalten erkennen, die im Zusammenhang mit Transportfunktionen der Kapillarendothelzellen des Pectenoculi diskutiert werden. Vermutlich spielt der Pecten eine wichtige Rolle für die Ernährung von Retina und Glaskörper.
    Notes: Summary The pecten oculi of the domestic chicken was examined with light, scanning and transmission electron microscopy and with freeze-etching techniques. Particular attention has been given to the capillary structure. The capillaries form an extensive anastomotic network. Their endothelial cells have apical (luminal), as well as basal, longitudinally oriented microfolds. It is assumed that the formation of apical differentiations of the endothelial surface is due to haemodynamic influences. Thus, sufficient surface area for membrane bound enzymes is achieved. These enzymes are necessary for active transcellular transport processes that require energy. In freeze-etched material, two different structures of the membrane surface of microfolds can be recognized. These results are discussed in relation to transport functions through capillary endothelial cells of the pecten. It is assumed that the pecten plays an important role in the nourishment of the retina and vitreous body.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Hoboken, NJ [u.a.] : Wiley-Blackwell
    Journal of Orthopaedic Research 4 (1986), S. 452-457 
    ISSN: 0736-0266
    Keywords: Spine ; Osteochondrosis ; Scheuermann's Disease ; Vertebra ; Electron microscopy ; Collagen fibers ; Collagen fibrils ; Life and Medical Sciences
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: In osteochondrosis juvenilis Scheuermann, foci of various sizes in the cartilaginous end plates of the vertebral bodies display a loosening or complete interruption of the collagen fibers. These findings, together with an alteration and occasional absence of the growth zone, may result in the typical deformation of the vertebral bodies. Electron micrographs of the areas with optically absent collagen fibers reveal collagen fibrils. They are arranged in an irregular pattern. We conclude that a disturbance of collagen or ground substance biosynthesis is of importance in the pathogenesis of juvenile osteochondrosis.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
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