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  • 1
    ISSN: 1432-055X
    Keywords: Schlüsselwörter Autologe Bluttransfusion ; Bundesrepublik Deutschland ; alte Bundesländer ; Key words Autologous blood transfusion ; Federal Republic of Germany ; “Old” federal states
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Abstract Increased perception of the risks of homologous blood transfusion has focused substantial interest on autologous blood transfusion (ABT). To evaluate the role of ABT in the Federal Republic of Germany (FRG), we conducted postal surveys of German hospitals in 1989 and 1993. In the present study, the responses of 143 hospitals in the “old” federal states (former West Germany) that replied to both of our surveys were analysed. The objective was to examine trends in ABT practice in the FRG from 1989 to 1993. The study was restricted to the “old” federal states because data for the “new” federal states (former German Democratic Republic) were not available for that period. Methods. Data presented in this study were obtained by postal surveys of German hospitals in 1989 and 1993. Because of German reunification (3 October 1990), only hospitals in the “old” federal states were surveyed in 1989, while hospitals in both the “old” and “new” states were included in 1993. Four hundred randomly selected hospitals in the “old” federal states were included in both surveys, 143 of which responded to both. The completed questionaires of these 143 hospitals were analysed. In addition, to estimate the magnitude of positive selection bias, the data provided by these 143 hospitals were compared with data provided by another set of 162 hospitals in the “old” federal states that replied to the 1993 but not to the 1989 survey. Results. There were no significant changes from 1989 to 1993 in the 143 hospitals with respect to hospital size by number of beds and spectrum of surgical specialties. The proportion of hospitals that performed preoperative autologous blood donation grew by 20% (from 74% to 89%) and the proportion that used this technique regularly (“frequently” and “mostly”) rose by 80% (from 25% to 45%). The percentage of hospitals that performed preoperative plasmapheresis had more than doubled (from 8% to 19%). The proportion that used acute isovolaemic haemodilution grew by 32% (from 62% to 82%), while those doing so regularly (“frequently” and “mostly”) increased by only 17% (from 23% to 27%). The fraction of hospitals equipped with cell-washing devices grew by 29% (from 35% to 45%). General transfusion practice, as measured by acceptance of postoperative haemoglobin levels of less than 10.0g/dl, had changed but little. Conclusions. Although the 143 hospitals are not representative on a national level, the findings of our study allow some conclusions on general trends in the “old” states of the FRG. The potential for ABT has substantially grown in the “old” states from 1989 to 1993, although more consistent advantage of this potential could be taken.
    Notes: Zusammenfassung In der Arbeit wird die jüngere Entwicklung der autologen Bluttransfusion in 143 Krankenhäusern aus den alten Bundesländern dargestellt. Die Daten stammen aus zwei in den Jahren 1989 bis 1993 von uns durchgeführten Fragebogenerhebungen. Der Anteil der Krankenhäuser, in denen eine präoperative Eigenblutspende möglich war, ist von 74% auf 89% und der Anteil der Krankenhäuser, in denen sie „häufig“ oder „meistens“ durchgeführt wurde, von 25% auf 45% angestiegen (20% bzw. 80% Zuwachs). Die Verbreitung der präoperativen Plasmapherese hat sich mehr als verdoppelt: 1989 wurde sie in 8% und 1993 in 19% der Krankenhäuser eingesetzt. Der Anteil der Krankenhäuser, in denen eine akute isovolämische Hämodilution durchgeführt wurde, ist von 62% auf 82% angestiegen (32% Zuwachs). Der Anteil der Krankenhäuser, in denen Zellseparatoren zur Verfügung standen, hat von 35% auf 45% zugenommen (29% Zuwachs). Die Transfusionspraxis hat sich, gemessen an der Akzeptanz postoperativer Hämoglobinwerte unter 10,0 g/dl, kaum gewandelt. Die Entwicklung der autologen Bluttransfusion in den 143 Krankenhäusern ist für die alten Bundesländer nicht repräsentativ. Sie gestattet aber Rückschlüsse auf die allgemeine Entwicklung der autologen Bluttransfusion in den alten Bundesländern.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Acta neuropathologica 1 (1961), S. 113-134 
    ISSN: 1432-0533
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary Three cases of a familial syndrome exhibiting dwarfism, testicular atrophy of male members, tapeto-retinal degeneration and a pathologic neurological constellation with extra-pyramidal and pyramidal disturbances beginning after puberty and showing a chronic, progressive development are described. The histo-pathological findings showed a diffuse, patchy demyelinization extending over the white matter of the hemispheres with involvement of white matter fibers of the cortex and the central grey masses. The products of the demyelinization, in the form of pre-lipoids or metachromatic substances were not demonstrable; the axial cylinders in the demyelinated areas remained, for the most part, unaffected. Rarification of the glia and emphasis of oligodendroglia in the demyelinated patches with occurrence of nuclear inclusion bodies in the glia are presented as new findings in the sense of formal pathological changes of the cellular apparatus whereby the ganglion cells remain, for the most part, intact. Keeping in mind the degenerative glial changes whose formal manifestations are evoluated upon the occurrence of nuclear inclusion bodies, the Pelizaeus-Merzbacher disease is seen as the expression of a special glial reaction form and is assigned to the other leuco-dystrophies which are classified according to the same pathophysiological points of view.
    Notes: Zusammenfassung Es wird bei drei Fällen ein familiäres Syndrom mit Zwergwuchs, bei den männlichen Gliedern mit Hodenatrophie, tapetoretinaler Degeneration und einem neurologischen Krankheitsbild mit extrapyramidal-pyramidalen Störungen mit Beginn nach der Pubertät und chronisch-progredientem Verlauf beschrieben. Der histopathologische Befund weist eine diffus-fleckförmig sich über das ganze Hemisphärenmark ausdehnende Entmarkung mit Miteinbezug der Markfasern der Rinde und der zentralen grauen Massen auf, wobei Myelinabbaustoffe in Form von Praelipoiden oder metachromatischer Substanz nicht nachweisbar und die Achsencylinder in den entmarkten Zonen weitgehend verschont sind. Das Krankheitsbild wird unter Zugrundelegung dieser wesentlichen morphologischen Kriterien derPelizaeus-Merzbacherschen Krankheit zugereiht. Als neuer Befund im Sinne formaler pathologischer Veränderungen des Zellapparates werden bei weitgehend erhaltenem Ganglienzellbestand die Rarefizierung der Glia und betont der Oligodendroglia in den entmarkten Flecken und das Inerscheinungstreten von Kerneinschlüssen in der Glia herausgestellt. Unter Berufung auf die zur Geltung kommenden degenerativen Gliaveränderungen, als deren formale Äußerung die Kerneinschlüsse gewertet werden, wird die Pelizaeus-Merzbachersche Krankheit als Ausdruck einer besonderen gliösen Reaktionsform den andern, nach gleichen pathophysiologischen Gesichtspunkten klassifizierten Leukodystrophien zugeordnet.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Acta neuropathologica 15 (1970), S. 142-155 
    ISSN: 1432-0533
    Keywords: Tri-Ortho-Cresyl Phosphate Poisoning ; Axonal Degeneration ; Synaptic Pathology ; Spinal Cord Biochemical Lesion ; EM CNS Pathology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Elektronenmikroskopische Untersuchungen des Rückenmarks von erwachsenen Hühnern, die mit TOCP (Tri-Ortho-Cresyl-Phosphat) vergiftet wurden, zeigen frühestens 1 Tag nach Auftreten der klinischen Symptome pathologische Veränderungen. Diese besitzen dieselbe Qualität wie im peripheren Nervensystem und betreffen die Nervenfasern der weißen Substanz in Form einer primären Axonerkrankung mit Proliferation des endoplasmatischen Reticulums und Desintegration der Neurofilamente. In der grauen Substanz der Vorderhörner sieht man besonders schwere strukturelle Veränderungen in den boutons terminaux, während die Motoneurone kaum verändert sind. Der auffallendste Befund schon im Frühstadium ist eine hochgradige Schwellung der sphärischen synaptischen Vesikel in den boutons desS-Typs, die den axosomatischen Synapsen zugehören.
    Notes: Summary Electron-microscopic investigations of the spinal cord in adult chickens poisoned with TOCP (Tri-Ortho-Cresyl Phosphate) show pathological alterations as early as the first day after appearance of the clinical symptoms. Of the same qualitative order as in the peripheral nervous system, they refer to the nerve fibres in the white matter in form of primarily diseased axons, with proliferation of the endoplasmic reticulum and disintegration of the neurofilaments. In the grey substance of the anterior horn, in particular, one sees severe structural alterations in the boutons terminaux while the motoneurons are hardly modified. The most striking finding already in the early stage is a massive swelling of the spherical synaptic vesicles in the boutons of theS-type, attributed to axo-somatic synapses.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Acta neuropathologica 39 (1977), S. 211-218 
    ISSN: 1432-0533
    Keywords: Thiamine deficiency ; Starvation ; Axonal degeneration ; Dying-back neuropathy ; Mitochondrial increase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Electron microscopic investigations on sciatic and plantar nerves of thiamine deficient and starved rats show a distally pronounced axonal degeneration. The changes are present in starved and thiamine deficient animals, but the deficient animals are more severely affected. The earliest alterations consist of an increase of mitochondria and a proliferation of vesicular elements of the endoplasmic reticulum. They are followed by loop formations of the axon membrane, clustering and disintegration of neurotubules and neurofilaments, axonal shrinkage and finally myelin disruption. The distal accentuation of the early changes indicates a dying-back mechanism of axonal degeneration.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1432-0533
    Keywords: Mucopolysaccharidosis II ; Prenatal diagnosis ; Electron microscopy ; Lysosomal storage
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Light and electron microscopic findings in the nervous system of a 23-week-old fetus are reported, in which MPS II was diagnosed prenatally. The degrees of myelination and neuronal differentiation were similar as in a normal fetus of the same age. A storage of mucopolysaccharides in typical vacuolar inclusion bodies was present throughout the peripheral and central nervous system, mainly in cells of mesenchymal origin. “Zebra” bodies and granulo-membranous bodies, which are thought to represent secondary ganglioside accumulation were only found in the well developed neurons of the spinal cord and spinal ganglia, but not in the poorly developed neurons of the cerebellar and cerebral cortex. Mucopolysaccharide storage in endothelial cells of cerebral blood vessels precedes the appearance of lipid storage in cerebral neurons.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Acta neuropathologica 8 (1967), S. 292-308 
    ISSN: 1432-0533
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary The case of a girl aged 3 is presented. Autopsy revealed a combination of amaurotic idiocy and metachromatic leucodystrophy. Histochemistry in the necroptic material showed storage of a glycolipid in the balloned cortical nerve cells and of granular material in the white matter. The latter reacts histochemically like a sulfatide, i. e. an acid gylcolipid different from the one in the cortex. It is associated with phospholipids and simple fats. Electron microscopy of a biopsy from the patient's sural nerve demonstrates the involvement of the peripheral nervous system. There is an accumulation of at least three different substances in the cytoplasm of the Schwann cells, two of which contain lipids. The membranous cytoplasmic bodies characteristic of amaurotic idiocy are not found. Therefore, in the peripheral nervous system, there are only the changes seen in metachromatic leucodystrophy. We conclude from these facts that there is a genetically induced combination of two different forms of lipidosis. There are also signs of an abnormal chemical structure of the otherwise normal myelin sheath revealed by an alteration of the lamellated myelin structure.
    Notes: Zusammenfassung Es wird der Fall eines dreijährigen Kindes vorgelegt, bei dem die Autopsie das Vorliegen einer Kombination von A.I. und MLD ergab. Die histochemische Analyse des Autopsiematerials bestätigt die Speicherung eines Glykolipids in den ballonisierten corticalen Ganglienzellen und daneben eines granulären Materials in der weißen Substanz, das sich als ein saures Glykolipid anderer Art als in der grauen Substanz in Gesellschaft von Phospholipid und niederen Lipoiden erweist. Die elektronenmikroskopische Untersuchung der Suralisbiopsie des Patienten zeigt den Befall des peripheren Nervensystems mit akkumulation mindestens dreier verschiedener, wovon zweier Lipoid-haltiger Substanzen im Cytoplasma der Schwannschen Zelle. Die für die A.I. typischen speicherkörper (MCB) finden sich hier nicht, so daß das periphere Nervensystem nur die Veränderungen wie bei der MLD erleidet. Es wird daraus auf eine genbedingte Kombination zweier verschiedener Lipoidosen geschlossen. Daneben bieten sich Anhaltspunkte für den abnormen chemischen Aufbau auch der sonst weitgehend unversehrt wirkenden markscheiden in Gestalt einer veränderten Markscheiden-Lamellenstruktur.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Acta neuropathologica 45 (1979), S. 221-229 
    ISSN: 1432-0533
    Keywords: Mouse mutant Jimpy ; Optic nerve ; Lipid ultrastructure ; Phagolysosomes ; Phagocytes ; Histochemistry ; Freeze-etching ; Thin sections
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Optic nerves of the mouse mutant Jimpy and controls were studied in different developmental stages. The results cast some light on the nature and ultrastructure of intracellular lipids, which are a striking feature in the CNS of these affected mice. Light Microscopy. In the premyelination period the activity of acid phosphatase is quite similar in the lysosomes of both animal groups. A much higher increase of reaction product was found in Jimpy compared to controls during the advanced myelination stages. The normal glial cell arrangement in rows is absent in leucodystrophic animals. Electron Microscopy. Replicas of freeze-etched Jimpy optic nerves show lipid loaded cells exhibiting a plasma membrane different from that of astrocytes and oligodendrocytes, thus suggesting a relationship to phagocyting microglia. Furthermore, the rare myelin membranes are almost particle free. Three types of structurally different lipid inclusions could be observed. Most of the lipidic bodies are surrounded by a membrane studded with particles. The freeze-fractured morphology of the lipid inclusions was compared with the appearance in thin sections, incubated for acid phosphatase demonstration. Acid phosphatase reaction product within all three types of lipid containing vesicles indicate the lysosomal nature of these structures, which may represent myelin breakdown products and/or myelin precursors.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Journal of molecular medicine 46 (1968), S. 666-671 
    ISSN: 1432-1440
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary A sural nerve biopsy was taken from a patient aged 28 suffering from Fabry's disease (Angiokeratoma corporis diffusum universale). It demonstrated that the peripheral nervous system is involved in a characteristic manner. Lipids were stored in the endothelial cells and in the pericytes of capillaris as well as in the cells of the perineural connective tissue. This lipid uniformly exhibited a cristalline structure characterized by a periodicity of 56–60 Å. Furthermore there were degenerated nonmyelinated axons. This latter finding is interpreted as a degenerative disease of the autonomic nervous system which would explain the sudorimotor insufficiency and the pains predominating the clinical picture.
    Notes: Zusammenfassung Die elektronenmikroskopische Untersuchung eines durch Biopsie gewonnenen Nervenstückes von einem an der Fabryschen Krankheit (Angiokeratoma corporis diffusum universale) leidenden 28jährigen Patienten zeigt, daß das periphere Nervensystem bei dieser Lipoidstoffwechselkrankheit in charakteristischer Weise mitbetroffen ist. Die Speicherung des Lipoids stellt sich elektiv in den Capillarendothelien und -pericyten sowie den Bindegewebszellen der Perineuralscheide dar. Das intracytoplasmatisch angereicherte Material weist dabei eine kristalline Binnenstruktur mit einer regelmäßigen Periodik von 56–60 Å auf. Daneben finden sich degenerierte unbemarkte Axone. Der letzte Befund, in Zusammen. hang mit dem klinischen Bild gebracht, muß als das morphologische Substrat des bestehenden Schweiß-Sekretionsdefektes und Schmerzsyndroms verstanden werden.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    European journal of clinical pharmacology 27 (1985), S. 721-725 
    ISSN: 1432-1041
    Keywords: alizapride ; cancer ; chemotherapy ; substituted benzamide ; nausea ; vomiting ; side-effects ; antiemetic therapy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Medicine
    Notes: Summary In early clinical trials alizapride showed a better antiemetic activity with fewer side effects than metoclopramide. Alizapride has now been evaluated in an open dose — ranging study in 24 patients receiving strongly emetic chemotherapy. Alizapride 4–8 mg/kg was given as a 15 min infusion 0.5 h before and 1.5, 3.5, 5.5 and 8.5 h after the chemotherapy. At the dose levels of 6 and 8 mg/kg × 5, respectively 6 out-of 9 and 4 of 4 patients experienced side effects (hypotension, dizziness, profuse sweating, general malaise and diarrhoea). At 4 mg/kg × 54 of 15 patients experienced side effects due to alizapride (dyspnoea 1, diarrhoea 2, extrapyramidal syndrome 1 patient). Overall, 9 of 24 patients were partially or completely protected from nausea and vomiting. Based on this experience alizapride has antiemetic activity and few side effects in the dose of 4 mg/kg × 5.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Acta neurochirurgica 9 (1961), S. 510-524 
    ISSN: 0942-0940
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary In material obtained from 90 cases of brain tumors verified histologically, the probability of finding tumor cells by means of an improved cytological diagnostic procedure of the liquor which employs a liquor sedimentation device was determined. Tumor cells were demonstrable in the liquor sediment in 32 cases. The greatest probability of finding tumor cells occurs with secundary brain tumors; amongst neuroepithelial tumors, Dest results were achieved with medulloblastomas and glioblastomas. The difficulties involved in the comparative cytological diagnosis of tumor cells, the limitations imposed by the procedure used, as well as the known biological principles governing tumor cell metastases in the liquor are discussed.
    Abstract: Résumé Dans 90 cas de tumeurs cérébrales histologiquement prouvées, une méthode améliorée de cytodiagnostic dans le liquide céphalo-rachidien (LCR) augmente de façon certaine la probabilité de révéler des cellules tumorales. Dans ce but on s'est servi d'un apparail spécial de sédimentation du LCR. Chez 32 patients des cellules tumorales ont pu être ainsi démontrées dans le sédiment du LCR. Ce sont les tumeurs secondaires du cerveau qui offrent les meilleures chances de rencontrer des cellules tumorales, et parmi les tumeurs neuro-épithéliales ce sont le médulloblastome et le glioblastome. Les difficultés du cytodiagnostic tumoral par comparaison, les limites qu'impose la méthode préconisée, et les principes biologiques qui régissent la dissémination de cellules tumorales dans le LCR sont discutées à la fin de ce travail.
    Notes: Zusammenfassung An einem histopathologisch gesicherten Krankengut von 90 Hirntumorfällen wurde mittels einer verbesserten liquorcytodiagnostischen Methode unter Anwendung eines Liquorsedimentiergerätes die Trefferwahrscheinlichkeit einer gezielten Tumorzellsuche bestimmt. Bei 32 der Fälle konnten Tumorzellen im Liquorsediment nachgewiesen werden. Die Trefferwahrscheinlichkeit erwies sich als am größten für sekundäre Hirngeschwülste. Unter den neuroepithelialen Tumoren war sie am beträchtlichsten für Medulloblastome und Glioblastome. Es werden die Schwierigkeiten der vergleichenden Tumorcytodiagnostik, die durch die Methodik bedingten Einschränkungen und die bekannten biologischen Grundlagen der Tumorzellmetastasierung in den Liquor diskutiert.
    Type of Medium: Electronic Resource
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