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  • Electronic Resource  (2)
  • Electrical Activity  (1)
  • Glomerulosklerose  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Pflügers Archiv 351 (1974), S. 195-206 
    ISSN: 1432-2013
    Keywords: Pancreatic Islets ; β-cells ; Electrical Activity ; Insulin Secretion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Intracellular microelectrode techniques have been used to investigate the membrane potential and electrical activity of β-cells of Langerhans islets of mice. In Krebs-Henseleit solution containing substimulatory glucose concentrations, i.e. concentrations which do not produce an insulin release, β-cells showed no electrical activity. In this concentration range an increase of glucose concentration depolarized the membrane without inducing spike activity. The β-cells became electrically active in glucose concentrations known to produce insulin secretion. The type of electrical activity depended on glucose concentration. Between 5.5 and 16.6 mM glucose it generally occured in bursts. Each was initiated by a depolarization of about 10 mV to a plateau level from which irregular spikes with frequencies up to 12/sec arose. The burst ended with a repolarization phase to the original potential level. With increasing glucose concentration the duration of the bursts increased and the intervals between the bursts shortened. Above 16.6 mM glucose the burst activity usually changed into continuous activity. If the relative duration of burst activity is plotted against glucose concentration there is a sigmoid relationship similar to that known to exist between insulin release and glucose concentration. TTX had no effect on burst activity. Removal of [Ca]o inhibited burst activity. Occasionally at zero [Ca]o action potential-like spikes were observed. Re-introduction of [Ca]o initially increased the frequency of these action potential-like spikes and then led to the usual glucose induced burst activity. The significance of the results is discussed in relation to the mechanism of insulin release.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Journal of molecular medicine 49 (1971), S. 1267-1271 
    ISSN: 1432-1440
    Keywords: non diabetic hyperinsulinism ; Kimmelstiel-Wilson's glomerulosclerosis ; Diabetes mellitus ; Glomerulosklerose ; Kimmelstiel-Wilson ; Glomerulonephritis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Es wird über den Krankheitsverlauf einer chronischen Glomerulonephritis mit nephrotischer Verlaufsform und schwerer Hypertonie berichtet, die histologisch die typischen Veränderungen einer intercapillären Glomerulosklerose Kimmelstiel-Wilson aufzeigte. Eine Glucosetoleranzstörung war bei dem Patienten mit Sicherheit ausgeschlossen. Es bestanden jedoch deutlich erhöhte Insulin-Konzentrationen. Dabei wird festgestellt, daß die für die Glomerulosklerose Kimmelstiel-Wilson als charakteristisch angesehenen Veränderungen auch ohne manifeste diabetische Stoffwechselstörung auftreten können. Es muß abgeklärt werden, ob dabei dem Insulin eine besondere Rolle zukommt oder ob es sich um unspezifische histologische Veränderungen handelt.
    Notes: Summary In a patient suffering of chronic glomerulonephritis with malignant hypertension, necropsy revealed histological changes considered to be typical for Kimmelstiel-Wilson's glomerulosclerosis. A disturbance of carbohydrate metabolism had been clearly excluded. However, the patient showed uremic hyperinsulinism. The findings suggest that histological changes regarded as typical for Kimmelstiel-Wilson's glomerulosclerosis may as well be found without any disturbances of carbohydrate metabolism. Experimental and clinical data suggest that insulin may be of special importance in the development of hyaline masses as seen in “diabetic glomerulosclerosis”.
    Type of Medium: Electronic Resource
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