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  • Electronic Resource  (4)
  • Functioning adenoma  (2)
  • Prasinophyceae  (2)
  • 1
    ISSN: 1432-0533
    Keywords: Horseradish peroxidase ; Concanavalin A ; Human pituitary adenoma ; Functioning adenoma ; Non-functioning adenoma
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Intracellular lectin (Con A)-binding sites of human pituitary adenoma were examined by electron microscopy using the horseradish-peroxidase (HRP)-labelling technique. In this study were used 16 cases of human pituitary adenomas operated on in our clinics between 1977 and 1981: they concluded 4 each of PRL-, GH-, ACTH-producing and hormonal non-functioning adenomas. In parallel with the detection of lectin-binding sites, basal levels of their secreting hormones were determined by the radioimmunoassay technique, and their producing hormones were characterized light microscopically by the immunocytochemical HRP-labelling technique. In the present study, for hormonal functioning adenoma cells, mature or large granules of each specific type of adenoma cells had no definite Con A-binding sites. On the other hand, immature or small secretory granules of RPL- or GH-producing adenoma cells showed a positive reaction with Con A. ACTH-producing adenomas so far examined revealed no definite binding sites. Some variable results were obtained concerning non-functioning adenomas. Lectins have been and will be very useful in the detection of different subtypes of adenomas in each specific group.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-0533
    Keywords: Ferritin labeled lectin ; Human pituitary adenoma ; Functioning adenoma ; Non-functioning adenoma
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Lectin-binding sites of the human pituitary adenoma cells were examined by electron microscopy in correlation with their functional states. In this study used were 37 cases of the human pituitary adenomas which had been operated in our clinics from 1977 to 1979. They were divided into four groups: 13 cases of PRL-producing adenomas, nine of hGH-producing adenomas, three of ACTH-producing adenomas, and 12 of hormonal non-functioning adenomas. In parallel with the detection of the lectinbinding sites by means of the ferritin-labeling method, the basal levels of their secreting hormones were determined by the radioimmunoassay technique, and their producing hormones were characterized light microscopically by the immunocytochemical HRP-labeling technique. The present study clearly shows that the ferritinlabeled, lectin-binding sites of the human pituitary adenoma cells prefixed with aldehyde are different in number between PRL- or hGH-producing adenoma cells and non-functioning ones. Morphologically resemblin, non-functioning adenoma cells and mixed somatotrophic and lactotrophic adenoma cells are definitely distinguished from each other by the findings of the ferritin-labeled RCA-binding sites' distribution pattern. Lectins have been proved very useful to evaluate the membrane characteristics of the human pituitary adenoma cells in association with their functional states.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Protoplasma 148 (1989), S. 41-56 
    ISSN: 1615-6102
    Keywords: Flagellar apparatus ; Connecting fibres ; Flagellar replication ; Numbering system of flagella ; Prasinophyceae ; Pyramimonas
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The flagellar apparatus of the octoflagellate prasinophytePyramimonas octopus has been examined in detail. While basal bodies of biflagellate green algae typically are interconnected by a proximal and distal fibre, the eight flagella ofPyramimonas octopus are joined together by at least 60 fibrillar structures, in this respect the most complex algal cell examined so far. The flagellar apparatus is asymmetrical, but part of it shows 180 degress rotational symmetry. We have detected 9 thick connecting fibres, c. 45 thinner fibres, 6 proximal fibres, one of which associates with only one basal body, and a large synistosome. Four 3-stranded microtubular roots are present, one of which associates with a cross-banded fibre leading to the aperture of the scale reservoir. Six rhizoplasts have been seen, but 8 are probably present. The association of each root and connecting fibre to individual (numbered) triplets of the basal bodies is described. The basal body system has been followed during flagellar replication. Newly formed flagella occupy different positions in the cell during 3 cell generations, but finally in the fourth generation end in the same central position near the nucleus. Based on the observed changes in position during successive generations, a numbering system for flagella in general is proposed.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1615-6102
    Keywords: Green algae ; Flagellar beat ; Video analysis ; Evolution ; Swimming patterns ; Prasinophyceae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The flagellar beat and swimming patterns of flagellated cells of 22 green plants, including 17 green flagellates (volvocalean and prasinophyte algae), motile cells of three seaweeds,Bryopsis, Caulerpa, andUlva, sperms of a liverwort,Marchantia, and a fern,Athyrium, were examined using a high-speed video system. So-called breast-stroke is widely distributed in green plants, and occurs rarely in prasinophyte flagellates and ulvophycean algae; in these algal groups flagellar beat similar to that found in animal sperm is common, both during forward and backward swimming. Different types of swimming patterns were observed in prasinophytes. The results indicate evolutionary trends of flagellar beat and swimming patterns in green plants such as change from backward to forward swimming, from flagellar to ciliary beating and from uni-directional (parallel) to radial-directional (cruciate) beating. Such trends are shown in two prasinophyte groups, thePyramimonas-lineage andTetraselmis-lineage.
    Type of Medium: Electronic Resource
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