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  • 1
    Electronic Resource
    Electronic Resource
    Secretion of plasminogen activator inhibitor by cultured human epidermal cells (1986)
    Oxford, UK : Blackwell Publishing Ltd
    British journal of dermatology 115 (1986), S. 0 
    Details
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: A conditioned medium from cultured human epidermal cells was observed to inhibit the activity of exogenous urokinase. By reverse fibrin autography after SDS polyacrylamide gel electrophoresis, a plasminogen activator inhibitor was detected with a molecular weight of 46000. Using Mr- 33000 [125I]-labelled urokinase we observed the formation of an enzyme-ligand complex. The molecular weight of this complex was 79000. These results indicate that cultured human epidermal cells secrete a plasminogen activator inhibitor (urokinase inhibitor) with a molecular weight of 46000.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2133.1986.tb05708.x
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  • 2
    Electronic Resource
    Electronic Resource
    Plasminogen activator secreted by cultured human melanocytes (1986)
    Oxford, UK : Blackwell Publishing Ltd
    British journal of dermatology 115 (1986), S. 0 
    Details
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: A plasminogen activator (PA), Mr 72 000, was detected in conditioned medium from human melanocyte cultures by fibrin autography. The electrophoretic mobility was identical to that of tissue PA produced by Bowes melanoma cells. PA activity in human melanocyte culture medium was inhibited by anti-tissue PA IgG, but not by anti-urokinase IgG. Our results are the first to show that normal human melanocytes in culture secrete tissue plasminogen activator.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2133.1986.tb05719.x
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  • 3
    Electronic Resource
    Electronic Resource
    The value of blood group-specific lectin and endothelial associated antibodies in the diagnosis of vascular proliferations (1986)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of cutaneous pathology 13 (1986), S. 0 
    Details
    ISSN: 1600-0560
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Blood vascular and lymphatic tumors were evaluated immunohistochemically by studying a spectrum of endothelial associated antigens. (1) UEA-I lectin reacted with the tumor cells of one patient with malignant angioendothelioma in the non-metastatic stage. However, when metastasis occurred, the binding sites of this lectin completely disappeared from the surface of the tumor cells in both original and metastatic lesions, suggesting the loss of blood group H antigen from the tumor cells could be used as an indicator of metastasis in this tumor. (2) Reaction with anti-HLA-A, B, C, intense in normal blood vessels, remained intensely positive in pyogenic granuloma and Kaposi's sarcoma, whereas it did not react with normal lymphatics and lymphangioma. This indicates that anti-HLA-A, B, C is useful in differentiating blood vascular structures from lymphatic structures in both normal and pathological conditions. (3) OKM5 reacted intensely with benign hyperplasius in pyogenic granuloma, while barely reacting with proliferating parts in Kaposi's sarcoma, suggesting the difference in staining patterns can be used to distinguish vascular proliferation or malignancy. (4) Reaction with anti-Type IV collagen and anti-laminin was intense in normal blood vessels, pyogenic granuloma and Kaposi's sarcoma, whereas reaction with these antibodies in normal lymphatics was patchy and irregular in its thickness.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0560.1986.tb01084.x
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  • 4
    Electronic Resource
    Electronic Resource
    Variation of the distribution pattern of SH groups and SS linkages in the human epidermis (1986)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of cutaneous pathology 13 (1986), S. 0 
    Details
    ISSN: 1600-0560
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Normal epidermis of various sites of human body was examined by N-(7-dimethyl-amino-4-methyl-3-coumarinyl)maleimidc (DACM) method for SH groups and SS linkages. The horny cells in the palm and sole showed a gradual occurrence of a SH membranous pattern, while those in other sites revealed an abrupt formation of the SH membranous pattern just above the granular layer. In the palm and sole epidermis the number of layers which exhibit membranous pattern of SH-positivity was thicker than that in the other epidermis; the low- through mid-layers of horny cells in the former sites seemed to contain not only SS-positive but also SH-positive substance at cell peripheries. The DACM staining for SS linkages showed the presence of a granular fluorescence in the cytoplasm of the granular cells of the epidermis in the human palm and sole, but not in other sites. The granular cells in human lip displayed a granular fluorescence in the cytoplasm by cither SH or SS DACM staining. All the SH- or SS-positive granular fluorescence was related to keratohyaline granules. These findings indicate that there may be some variations of the distribution pattern of the epidermal cellular proteins with SH and/or SS and a difference in chemical composition of keratohyaline granules between different anatomical sites of human body.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0560.1986.tb01524.x
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  • 5
    Electronic Resource
    Electronic Resource
    Elimination of Neutrophils by Apoptosis During the Resolution of Acute Pulmonary Inflammation in Rats (1998)
    Springer
    Lung 176 (1998), S. 89-98 
    Details
    ISSN: 1432-1750
    Keywords: Key Words: Neutrophils—Apoptosis—Macrophages—Ozone—TUNEL.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. We evaluated the apoptosis of neutrophils during the resolution of acute pulmonary inflammation induced by exposure to ozone. The inflammatory response was assessed in rat lungs 0, 1, 3, and 7 days after 4-h exposure to air or 2 ppm ozone. Analysis of bronchoalveolar lavage fluid demonstrated significant increases in albumin concentrations on days 0 and 1 and in the number of lavageable neutrophils on days 0, 1, and 3, indicating the presence of acute pulmonary inflammation. These parameters returned to control values on day 7, which suggests that the acute pulmonary inflammation induced by ozone was reversible. On days 1 and 3, but not on day 0, the neutrophils showed morphologic evidence of apoptosis. Based on morphologic analysis, the proportion of apoptotic neutrophils was 23.3 ± 2.2% on day 1 and 55.7 ± 3.2% on day 3. Terminal deoxynucleotidyl transferase-mediated dUTP end labeling (TUNEL), in contrast, revealed that the proportion of apoptotic cells was 59.7 ± 9.1% on day 1 and 68.0 ± 4.3% on day 3. On day 3, light microscopy and electron microscopy demonstrated engulfment of the neutrophils by macrophages. These findings indicate that the apoptosis of neutrophils followed by their engulfment by macrophages contributes to the clearance of neutrophils from the sites of inflammation. Moreover, TUNEL detected apoptotic neutrophils with greater sensitivity compared with morphologic assessment.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/PL00007597
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  • 6
    Electronic Resource
    Electronic Resource
    Preparation and Sintering of SiC Particulate Dispersed Al〈sub〉2〈/sub〉O〈sub〉3〈/sub〉 Fine Powders by Spray Pyrolysis Method (1998)
    s.l. ; Stafa-Zurich, Switzerland
    Key engineering materials Vol. 161-163 (July 1998), p. 407-410 
    Details
    ISSN: 1013-9826
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/45/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.161-163.407.pdf
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  • 7
    Electronic Resource
    Electronic Resource
    Perivascular T cells are infected with HTLV-I in the spinal cord lesions with HTLV-I-associated myelopathy/tropical spastic paraparesis: double staining of immunohistochemistry and polymerase chain reaction in situ hybridization (1998)
    Springer
    Acta neuropathologica 96 (1998), S. 340-346 
    Details
    ISSN: 1432-0533
    Keywords: Key words Immunohistochemistry ; Polymerase chain ; reaction in situ hybridization ; HTLV-I-associated ; myelopathy/tropical spastic paraparesis ; Double ; staining ; Fresh frozen sections
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract HTLV-I-infected cells play an important role in pathogenesis HTLV-I-associated myelopathy/tropical spastic paraparesis (HAM/TSP). Our previous studies of quantitative polymerase chain reaction (PCR) and in situ PCR suggested that T cells infiltrating in the spinal cord lesion were infected with HTLV-I. To elucidate the localization of HTLV-I proviral DNA directly, we performed double staining using immunohistochemistry and PCR in situ hybridization (PCR-ISH). Fresh frozen sections of the spinal cord from four HAM patients taken at autopsy were first immunostained with antibodies to pan T cells (UCHL-1), macrophages (KP-1) and helper/inducer T cells (OPD4). Then PCR-ISH was carried out with specific primers and probe for the HTLV-I pX region. UCHL-1-positive cells were noted around perivascular areas and, to some extent, in the parenchyma. Of the UCHL-1-positive cells, 9.4% (case 1), 9.6% (case 2), 1.1% (case 3) and 6.7% (case 4) became positive in HTLV-I PCR-ISH. UCHL-1-negative cells were HTLV-I PCR-ISH negative and almost all KP-1-positive cells were HTLV-I negative. HTLV-I was localized to OPD4-positive cells in examined lesions of cases 2 and 4. These data are a direct demonstration of HTLV-I proviral DNA localizing to infiltrated T cells in HAM/ TSP spinal cord lesions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004010050903
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  • 8
    Electronic Resource
    Electronic Resource
    Bioactive marine metabolites. VIII. Isolation of an antimicrobial blue pigment from the bryozoanBugula dentata (1986)
    Springer
    Cellular and molecular life sciences 42 (1986), S. 84-84 
    Details
    ISSN: 1420-9071
    Keywords: Bugula dentata ; bryozoa ; antimicrobial ; tetrapyrrole
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary An antimicrobial blue pigment, which is identical with a tetrapyrrole from a bacterium, was isolated from the bryozoanBugula dentata.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01975907
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  • 9
    Electronic Resource
    Electronic Resource
    Okinonellins A and B, two novel furanosesterterpenes, which inhibit cell division of fertilized starfish eggs, from the marine spongeSpongionella sp (1986)
    Springer
    Cellular and molecular life sciences 42 (1986), S. 1299-1300 
    Details
    ISSN: 1420-9071
    Keywords: Spongionella sp. ; porifera ; cell division inhibitor ; starfish embryos ; furanosesterterpene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Two novel furanosesterterpenes, okinonellins A (1) and B (2), have been isolated from the spongeSpongionella sp. Both compounds inhibit cell division of starfish embryos.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01946432
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  • 10
    Electronic Resource
    Electronic Resource
    AFM observation of surface reconstruction of C60 single crystals (1998)
    Springer
    Applied physics 66 (1998), S. S763 
    Details
    ISSN: 1432-0630
    Keywords: PACS: 61.48.+c; 68.35.-p; 68.35.Bs
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: 60 single crystals. The (111) and the (001) faces of C60 single crystals were illuminated with photons with energies below the band gap (generation of Frenkel-type excitons) or above the band gap. In the case of sub-band-gap illumination, reconstructed surfaces formed on the crystals. The reconstruction was represented by fringes running in the [11 ] and [0 0] directions on the (111) and (001) surfaces, respectively. As a result of supra-band-gap illumination, however, there appeared only cracks, without any fringes, which were oriented in directions characteristic of each face. The results suggest that the photo-induced surface reconstruction of C60 single crystals is induced via the recombination of Frenkel-type excitons.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003390051238
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