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  • 1985-1989  (3)
  • 1987  (3)
Material
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  • 1985-1989  (3)
Year
  • 1
    Electronic Resource
    Electronic Resource
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 7 (1987), S. 315-322 
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The object of the presented studies was to determine the conditions of protein biosynthesis from lignocellulosic raw material using filamentous fungi.It was found that on beet pulp + 50% wheat straw, strain A. niger (A.n./61) shows the highest effectivity of protein biosynthesis. Using this strain, after 48-hour incubation the following results were obtained: increase in protein 4.43 g/100 g·DM, daily protein increase coeffizient 2.22 g/100 g DM x 24 h, protein yield (based on the decrease in dry matter) 22.3% and cellulose utilization 37%.Upon use of a new strain CEL 2 and milled wheat straw as the substrate, the best results obtained after 74-hour incubation were as follows: increase in protein 1.82 g/100 g DM, daily protein increase coefficient 0.61 g/100 g DM x 24 h, protein yield 15,8% and cellulose utilization 22.0%.For rape straw and strain Sporotrichum pulverulentum, after 192-hour cultivation the following results were obtained: increase in protein 5.4 g/100 g DM, daily protein increase coefficient 0.68 g/100 g DM x 24 h, protein yield 14% and cellulose utilization 50.2%.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The influence of long-term storage on the stability of the cellulolytic enzyme and protein biosynthesis ability, was determined.Microorganisms belonging to genera Aspergillus, Fusarium, Penicillium, Sporotrichum, Allescheria and Mucor were used. The strains were stored on wort agar slants, on SAUNDER'Smedium and on a medium according to MANDELS-WEBER, under paraffin oil or without it. After 3 years storage no influence of the storage method on the biosynthesis of cellulolytic enzymes (strain A. niger 33/2 and 33/20) was found. A systematic increase in the celluloslytic activity was exhibited by strains A.n. 33/2 and A.n. 33/20 on wort agar (40 and 98%, respectively) and on SAUNDER'Smedium (79 and 66%, respectively), as well as by the new strain CEL 2 on Saunder's medium without paraffin oil.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 7 (1987), S. 327-330 
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Growth kinetics of Aspergillus niger strain A.n./61 on a solid medium containing beet pulp were examined.Solid state fermentation was carried out in laboratory microfermenter (aerated packed columns). During the experiments the CO2 evolution, protein biosynthesis, as well as cellulose and sucrose utilization were determined.It was found that CO2 evolution evidently increased in the logarithmic phase, together with the protein biosynthesis. Between 30-32 h of culture CO2 evolution and protein content in product were the greatest. Subsequently, CO2 evolution decreased.During 28 h of culture, cellulose was slightly utilized, the strain using mainly sucrose. Intense utilization of cellulose was paralleled by a drop in CO2 evolution.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
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