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  • 1
    Electronic Resource
    Electronic Resource
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 8 (1988), S. 349-355 
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The aim of the investigations was to obtain  -  from the parent strain Aspergillus niger A.n. 33  -  selectants with an increased ability of cellulolytic enzymes biosynthesis. Own selection methods allowed to receive two selectants A.n. 33/2 and A.n. 33/20 characterized by enhanced activities of saccharifying cellulase (respectively 0.11 and 0.14 FPU/cm3), endo-beta-1,4-glucanase (15.4 and 21.8 U/cm3) and cellobiase (0.6 and 1.4 IU/cm3) as compared with the parent strain (FPA  -  0.09 IU, CMC  -  8.2 U and CB  -  0.1 IU/cm3). Moreover, the selectants differed in shape and size of conidial heads, in shape and colour of conidia, as well as in structure and shape of hyphae. Enzyme preparations obtained after ultrafiltration of liquid cultures were characterized by following activites: FPA-4-16 IU, CMC  -  900-1800 U, CB  -  60-120 IU and xylanase  -  250-280 IU/cm3.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The influence of long-term storage on the stability of the cellulolytic enzyme and protein biosynthesis ability, was determined.Microorganisms belonging to genera Aspergillus, Fusarium, Penicillium, Sporotrichum, Allescheria and Mucor were used. The strains were stored on wort agar slants, on SAUNDER'Smedium and on a medium according to MANDELS-WEBER, under paraffin oil or without it. After 3 years storage no influence of the storage method on the biosynthesis of cellulolytic enzymes (strain A. niger 33/2 and 33/20) was found. A systematic increase in the celluloslytic activity was exhibited by strains A.n. 33/2 and A.n. 33/20 on wort agar (40 and 98%, respectively) and on SAUNDER'Smedium (79 and 66%, respectively), as well as by the new strain CEL 2 on Saunder's medium without paraffin oil.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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