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  • 1
    Electronic Resource
    Electronic Resource
    High-resolution time-of-flight methods for studies on ionic fragmentation of molecules : INTERNATIONAL CONFERENCE ON SYNCHROTRON RADIATION INSTRUMENTATION (1989)
    [S.l.] : American Institute of Physics (AIP)
    Review of Scientific Instruments 60 (1989), S. 2193-2196 
    Details
    ISSN: 1089-7623
    Source: AIP Digital Archive
    Topics: Physics , Electrical Engineering, Measurement and Control Technology
    Notes: The design and operation of the time-of-flight (TOF) mass spectrometer developed for ionic photofragmentation studies of molecules is described. Characteristics are summarized for three different operation modes of the TOF spectrometer; a pulse mode, a photoelectron–photoion coincidence mode, and a photoion–photoion coincidence mode. Experimental results for SiH4 are demonstrated as an example for the performance of the apparatus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.1140819
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  • 2
    Electronic Resource
    Electronic Resource
    Binding Properties of Human Anti-DNA Antibodies to Cloned Human DNA Fragments (1989)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 30 (1989), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The DNA–anti-DNA antibody immune complexes were isolated from plasma of systemic lupus crythematosus (SLE) patients and DNA fragments separated from immune complexes were subjected to molecular cloning. The resulting recombinant DNA clones showed a molecular size of 37–79 base pairs, a high guanine and cytosine content, high frequencies of CpG dinucleotides, and palindromic sequences, and also clusters of G+C- and A+T-rich segments. These clones hybridized randomly to total human DNA. The reactivity of dsDNA antibodies, both monoclonal and polyclonal, from SLE was examined with a cloned SLE antigen DNA. A competitive inhibition assay showed that human monoclonal antibodies had at least one magnitude higher affinity to the cloned DNA than to the native DNA fragments. In order to characterize the factors that were recognized by antibodies, human G+C-rich and also A+T-rich 100 bp DNA fragments were cloned, and their base sequences determined. The antibody showed a higher affinity to the G+C-rich DNA fragment (71% G+C) than to the A+T-rich DNA fragment (46% G+C). When cytosines in CpG doublets in G+C-rich fragments were methylated (mCpG), the reactivity increased up to 100-fold. The native anti-DNA antibodies from SLE patients also showed preferential binding to G+C-rich fragments. These observations suggested that human anti-dsDNA antibodies may recognize some unique structures around the G+C regions or G+C clusters of DNA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1989.tb01188.x
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  • 3
    Electronic Resource
    Electronic Resource
    Rapid increase of prostaglandin F2-alpha in neurons after subarachnoid hemorrhage in rats: an immunohistochemical study (1989)
    Springer
    Acta neuropathologica 78 (1989), S. 621-628 
    Details
    ISSN: 1432-0533
    Keywords: Subarachnoid hemorrhage ; Prostaglandin F2-alpha ; Hippocampus ; Purkinje cell ; Intracranial hypertension
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The effects of subarachnoid hemorrhage (SAH) with various degrees of increase in intracranial pressure (ICP) on the staining of prostaglandin F2-alpha (PG F2α) were studied in rat brains. SAH was produced in 18 rats by injection of 0.18–0.20 ml of autologous arterial blood/100 g body weight into the cisterna magna. By changing the speed of injection, the ICP was transiently increased by 346±68 (mean±S.D.) mm Hg in eight rats (including three pretreated with indomethacin), by 200±42 mm Hg in five rats, and by 6±4 mm Hg in the other five. Three rats injected with the same volume of mock cerebrospinal fluid (CSF) with ICP increased by 217±67 mm Hg and five normal rats without injection served as controls. All animals were decapitated 15 min after injection. The cryosections were stained for PG F2α using an indirect immunofluorescence method. Positive staining for PG F2α was noted only in pial vessels in all normal and mock-CSF-injected rats. In SAH rats with ICP increased by 6±4 mm Hg, there was a positive reaction in hippocampal neurons and Purkinje cells as well as blood vessels. SAH rats with higher ICP showed stronger PG F2α staining in the above areas, as well as in cerebellar granule cells. All rats pretreated with indomethacin showed a smaller increase in staining. The above results indicate that subarachnoid blood clots per se produce a rapid increase of PG F2α in neurons and blood vessels of both cerebrum and cerebellum, and that this increase is augmented by intracranial hypertension.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00691289
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  • 4
    Electronic Resource
    Electronic Resource
    Plakorin, a potent Ca2+-ATPase activator from the Okinawan marine spongePlakortis sp. (1989)
    Springer
    Cellular and molecular life sciences 45 (1989), S. 898-899 
    Details
    ISSN: 1420-9071
    Keywords: Sponge ; plakorin ; Plakortis sp. ; cyclic peroxide ; Ca2+-ATPase activator
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A new cyclic peroxide, plakorin, which is a potent sarcoplasmic reticulum (SR) Caa+-ATPase activator has been isolated from the Okinawan marine spongePlakortis sp., its structure was elucidated on the basis of spectral data.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01954069
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  • 5
    Electronic Resource
    Electronic Resource
    Effect of hypoxia on endothelium-dependent relaxation of canine and rabbit basilar arteries (1989)
    Springer
    Acta neurochirurgica 97 (1989), S. 77-82 
    Details
    ISSN: 0942-0940
    Keywords: Endothelium-dependent relaxation ; hypoxia ; subarachnoid haemorrhage ; vasospasm
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary An important role of endothelium-dependent relaxation in the local regulation of vascular tone has been suggested. In the present study, the effect of hypoxia on endothelium-dependent relaxation was investigated in canine and rabbit basilar and in rabbit common carotid arteriesin vitro, using an isometric tension recording method. Hypoxia was introduced by changing the gas mixture in thein vitro chamber from 95% O2-5% CO2 to 95% N2-5% CO2. Thrombin and acetylcholine were used to induce endothelium-dependent relaxation. Thrombin at 0.1 and 1.0U/ml, respectively, caused dose-dependent relaxation of the canine basilar artery precontracted by 10−6M prostaglandin F2α. Acetylcholine also evoked dose-dependent relaxation of rabbit basilar and common carotid arteries precontracted by serotonin. Under hypoxic conditions, the relaxing effect of thrombin or acetylcholine decreased both in canine and in rabbit arteries, although it was not significant in rabbit basilar arteries. It has been postulated that following subarachnoid haemorrhage, diffusion of oxygen to the walls of the major cerebral arteries might be impaired by the subarachnoid clot. This could cause hypoxia of the arteries and contribute to vasospasm by suppressing endothelium-dependent relaxation, as well as by enhancing the contractile responses of the cerebral arteries to the vasoconstrictor agents in the bloody cerebrospinal fluid.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01577744
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  • 6
    Electronic Resource
    Electronic Resource
    Time course of the blood-arterial wall barrier disruption following experimental subarachnoid haemorrhage (1989)
    Springer
    Acta neurochirurgica 98 (1989), S. 176-183 
    Details
    ISSN: 0942-0940
    Keywords: Cerebral vasospasm ; subarachnoid haemorrhage ; vascular permeability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The time course of the blood-arterial wall barrier disruption following experimental subarachnoid haemorrhage (SAH) was studied in 24 rabbits. Animals with SAH received two successive blood injections through the cisterna magna. Horseradish peroxidase (HRP) was given intravenously 30 minutes before sacrifice to assess the integrity of the barrier. In the basilar arteries taken from animals that were sacrificed 4 days after the first SAH, HRP-reaction products were diffusely observed in the subendothelial space. Three weeks following the first SAH, permeation of HRP was still observed in half of the animals. However, in animals sacrificed 7 weeks after the first SAH, no permeation of HRP into the subendothelial space was noted. Opening of the interendothelial space seemed to be the major mechanism for HRP permeation into the subendothelial space rather than transendothelial vesicular transport. Disruption of the bloodarterial wall barrier in the major cerebral arteries following SAH may play a role in the pathogenesis of vasospasm.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01407345
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  • 7
    Electronic Resource
    Electronic Resource
    Blood-arterial wall barrier disruption to various sized tracers following subarachnoid haemorrhage (1989)
    Springer
    Acta neurochirurgica 99 (1989), S. 76-84 
    Details
    ISSN: 0942-0940
    Keywords: Subarachnoid haemorrhage ; cerebral vasospasm ; vascular permeability ; FITC-dextran
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Disruption of the blood-arterial wall barrier in the major cerebral arteries occurs following subarachnoid haemorrhage (SAH) and may be related to the pathogenesis of cerebral vasospasm. Using FITC dextrans of various sizes, the present study was undertaken to determine if the barrier disruption shortly after SAH occurs equally to various sized tracers. Forty-two Sprague-Dawley rats were divided into 5 groups. Four groups were injected with FITC-dextrans of differing molecular weights (MW): FD4 (MW=4,080), FD40 (MW=40,500), FD 70 (MW=71,400), and FD 150 (MW=156,900). One group was injected with horseradish peroxidase (HRP: MW=40,000). Each group was further divided into two subgroups: with or without SAH. SAH was induced by injecting arterial blood into the cisterna magna. To assess the integrity of the blood-arterial wall barrier by transmission electron microscope, the tracers were intravenously injected prior to sacrificing the animals. The groups without SAH showed no permeability of tracers into the subendothelial spaces of the basilar arteries. In contrast, with the exception of FD 150, FITC-dextrans (FD 4, FD 40, FD 70) were noticed in the subendothelial spaces. The distribution of FITC-dextrans in the elastic lamina was similar to that of HRP. These results suggest that barrier disruption occurs with a wide range of molecular sizes of FITC-dextrans, although there seems to be some limitation to the permeation of the larger molecules. The present study suggests that the mechanism of barrier disruption of the major cerebral arteries in the acute stage following SAH may be vesicular rather than by separation of tight junctions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01407780
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  • 8
    Electronic Resource
    Electronic Resource
    Operation for the stiff elbow (1989)
    Springer
    International orthopaedics 13 (1989), S. 263-268 
    Details
    ISSN: 1432-5195
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Résumé Quarante-deux cas de raideur du coude ont été traités chirurgicalement. Parmi cux, 33 cas, etaient post-traumatiques, 7 cas étaient dûs à une ossification ectopique (OE) après quadriplégie et 2 cas étaient une OE importante après un coma prolongé dû à un traumatisme crânien. Deux incisions, l'une postéro-interne ou interne, l'autre antérieure ont été utilisées dans la plupart des cas. Dans les cas post-traumatiques, l'élément pathologique le plus caractéristique était la présence de fibres épaisses, obliques postérieures, au niveau du ligament latéral interne (LLI). Les ossifications ectopiques étaient localisées dans la plupart des cas dans la région postéro-interne du coude. La résection de ces LLI et/ou de l'ossification ectopique était le procédé le plus efficace pour améliorer la flexion du coude. L'amélioration moyenne de la mobilité a été de 58,8° dans les cas post-traumatiques, de 72,1° dans les cas d'OE après quadriplégie, et de 127,5° dans les cas d'OE due au coma.
    Notes: Summary Forty two cases of stiff elbow were treated by operation. Thirty three were subsequent to trauma, seven were due to ectopic ossification after quadriplegia, and two were associated with massive ectopic ossification after long term coma following head injury. Two separate skin incisions, postero-medial or medial and anterior, were used in most cases. In the post-traumatic cases, scarred, thickened posterior oblique fibres of the medial collateral ligament were usually found. Ectopic ossification was frequently situated on the postero-medial aspect of the stiff elbows. Resection of the thickened fibres or the ectopic ossification was the most effective procedure to improve elbow flexion. The average improvement in range of motion was 59° in post-traumatic cases, 72° in ectopic ossification after quadriplegia and 127° in ectopic ossification following coma.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00268509
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