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  • 1995-1999
  • 1990-1994  (3)
  • 1990  (3)
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Years
  • 1995-1999
  • 1990-1994  (3)
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Keywords
  • 1
    Electronic Resource
    Electronic Resource
    Molecular cloning of feline leukemia provirus genomes integrated in the feline large granular lymphoma cells (1990)
    Springer
    Archives of virology 111 (1990), S. 177-185 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Feline leukemia virus (FeLV) is a horizontally transmitted agent of the domestic cat which is known to be associated with wide spectrum of diseases of the hematopoietic system. In the present study, proviral DNAs of FeLV proviruses were examined in the tumor cells of natural killer cell lineage which is very rare in cats. In the chromosomal DNA of the tumor cells, 5 distinct bands corresponding to exogenous FeLV provirus genomes were detected by digestion withEcoRI which does not cut most FeLV isolates. Five clones of pLC1, pLC2, pLC3, pLC4, and pLC5 obtained from the 5 respective bands were analysed by restriction endonuclease mapping and Southern blot hybridization using gene-specific probes of FeLV. The results have clearly demonstrated that pLC4 and pLC5 contained large deletions in thepol and part ofgag regions, while the full-length proviruses could be observed in pLC1 and pLC2. Furthermore, pLC3 contained part of a variant FeLV genome having anEcoRI site in itsgag region. The molecular clones of defective and variant FeLV in this study may be useful for the further examination of tumorigenesis of large granular lymphoma in the cat.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01311052
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Effect of carbamylcholine on Harderian gland morphology in rats (1990)
    Springer
    Cell & tissue research 261 (1990), S. 451-459 
    Details
    ISSN: 1432-0878
    Keywords: Harderian gland ; Ultrastructure ; Morphometry ; Carbamylcholine ; Secretion ; Rat (Slc: SD)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary To determine the effect of cholinergic secretagogue on the Harderian gland of rats, several light- and electron-microscopic parameters were morphometrically assessed at different time intervals after carbamylcholine injection. In controls, two types of glandular cells (type A cells having 40–55 large vacuoles per cell profile and type B cells containing 30–38 smaller vacuoles per cell profile) and myoepithelial cells were recognized. At 5 min after injection of carbamylcholine, when rats secreted “bloody tears”, many alveoli showing narrower lumina and exocytotic figures in both types of cells were observed. Some vacuoles, which were covered by thin cytoplasmic sheets, protruded into the alveolar lumina. However, there was no evidence of apocrine or holocrine secretion. At 30 min and 120 min after injection, most of the alveolar lumina were dilated, and a pronounced decrease in the number of vacuoles in the glandular cells was observed. At 300 min after injection, the secretory vacuoles in both cell types reaccumulated. Transitional forms between the two cell types were not observed. The two types of Harderian gland cells can therefore be considered independent populations rather than different secretory stages of the same cell. It appears that the secretory process of the Harderian gland of rat is affected by cholinergic stimulation of the two types of glandular cells and of myoepithelial cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00313523
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    A cation-exchange study of stability constants of complexes formed between bismuth/III/ and nitrate or chloride ions (1990)
    Springer
    Journal of radioanalytical and nuclear chemistry 145 (1990), S. 167-173 
    Details
    ISSN: 1588-2780
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract A cation-exchange method was used for determining the stability constants of complexes formed between Bi/III/, present in tracer concentrations /ca. 10−11M Bi/, and nitrate ion or chloride ion in 1.OM H/ClO4, NO3/ or 1.OM H/Cl, ClO4/ solutions, respectively. The successive formation constants were calculated using the distribution data. The resulting 1g β1, 1g β2, and 1g β3 values were 0.74, 1.22, and 1.54 for 1.OM H/ClO4, NO3/ solutions, and 2.36, 3.61, and 4.95 for 1.OM H/Cl, ClO4/ solutions, respectively.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02202021
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    Paper (German National Licenses)
    Fulltext
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