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  • 1
    Electronic Resource
    Electronic Resource
    Interleukin-1β stimulates collagenase production by cultured human periodontal ligament fibroblasts (1994)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of periodontal research 29 (1994), S. 0 
    Details
    ISSN: 1600-0765
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: To examine the effects of interleukin-lβ (IL-1β) on collagenase production by human periodontal ligament fibroblasts (PLF) and gingival fibroblasts (GF) in Culture, collagenase activity in conditioned media was determined using a novel procedure that circumvented interference by enzyme inhibitors. Fibroblasts obtained from five paired periodontal ligament and gingival tissues were cultured for two weeks, and then incubated for a further 72 h in α-MEM supplemented with various concentrations of IL-1β (0 to 1250 pg/ml). The conditioned media from individual cultures were harvested and treated with dithiothreitol to inactivate TIMPs, and then with APMA, to activate the latent collagenase. Collagenase activity was measured fluorometrically using FITC-collagen as a substrate. IL-lβ induced a ∼2.4 to 5.2-fold increase in collagenase activity in PLF compared to a ∼1.4 to 2.2-fold increase in GF. These results are in contrast to previous studies in which collagenase activity was measured in the presence of TIMPs, and indicate that PLF are more sensitive to IL-1β than GF. Since both PLF and GF are present in periodontal lesions, it is possible that collagenase secretion stimulated by exposure to inflammatory cell products such as IL-lβ may participate in the destruction of collagen fibers involved in periodontal attachment.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0765.1994.tb01244.x
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  • 2
    Electronic Resource
    Electronic Resource
    Superlattice Structure in Al-Ni-Co Decagonal Quasicrystal (1994)
    s.l. ; Stafa-Zurich, Switzerland
    Materials science forum Vol. 150-151 (Jan. 1994), p. 223-234 
    Details
    ISSN: 1662-9752
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/02/00/transtech_doi~10.4028%252Fwww.scientific.net%252FMSF.150-151.223.pdf
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  • 3
    Electronic Resource
    Electronic Resource
    A novel synthetic vitamin D analogue, 2β-(3-hydroxypropoxy)1α, 25-dihydroxyvitamin D3 (ED-71), increases bone mass by stimulating the bone formation in normal and ovariectomized rats (1994)
    Springer
    Calcified tissue international 54 (1994), S. 142-149 
    Details
    ISSN: 1432-0827
    Keywords: Normal and ovariectomized rats ; Lumbar vertebra ; Bone formation ; Histomorphometry ; 2β-(3-hydroxypropoxy)-1α,25(OH)2D3
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Abstract We performed dosing experiments to evaluate the bone mass increasing action of a novel, synthetic vitamin D derivative, 2β-(3-hydroxypropoxy)-1α, 25(OH)2D3 (ED-71), in normal and estrogen-deficient rats. The first experiment consisted of 31 Sprague-Dawley rats, 28 weeks of age. The second experiment consisted of 44 animals who were ovariectomized (OVX) or sham operated at the age of 12 weeks. ED-71 was given twice a week for the duration of 12 weeks. At the end of the experiments, serum chemistries were examined and lumbar vertebrae were assessed histomorphometrically. Serum alkaline-phosphatase levels tended to decrease by ED-71 administration in the first experiment and their elevated values after ovariectomy were also depressed by ED-71 in the second experiment. Serum osteocalcin levels, however, increased by the agent. In the first experiment, cancellous bone volume (BV/TV) increased dose dependently. Bone formation rates (BFR/BS) also increased. In the second experiment, BV/TV significantly decreased by ovariectomy and it increased in ED-71-treated groups, but not in 1α-(OH)D3-treated group. BFR/BS increased by ED-71. Activation frequency did not decreased by ED-71 in either experiment. These data clearly demonstrated that ED-71 administration was capable of increasing the bone mass by stimulating bone formation in normal and estrogen-deficient rats.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00296065
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  • 4
    Electronic Resource
    Electronic Resource
    Spacio-temporal progression of demyelination in twitcher mouse: with clinico-pathological correlation (1994)
    Springer
    Acta neuropathologica 88 (1994), S. 228-236 
    Details
    ISSN: 1432-0533
    Keywords: Key words Globoid cell leukodystrophy ; Myelination ; Luxol fast blue-PAS ; Myelin basic protein ; Glial fibrillary acidic protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The twitcher (twi/twi) is an authentic murine model of human globoid cell leukodystrophy (GLD), caused by a deficiency of galactosylceramidase. Similar to human GLD, the twitcher shows progressive deterioration of neurological function and its neuropathology is characterized by a collection of periodic acid-Schift stain (PAS)-positive macrophages in the areas of demyelination. However, there are some differences in the clinico-pathological aspects between human and murine GLD. We investigated the spacio-temporal progression of neuropathology in the twitcher from postnatal day (PND) 10 to 45. No clinical symptoms or neuropathological changes were apparent in twi/twi until PND 15. Generally, infiltration of macrophages, concomitant with myelin degeneration, was recognized in the cerebellar white matter and the brain stem after PND 20, then in cerebral white matter after PND 25, and in cerebral and cerebellar gray matter after PND 30. The demyelination was very severe in the radix of the 8th and the 5th cranial nerves. The neurological symptoms such as tremor, spasticity and cranial nerve dysfunction were well correlated with the progression of pathological changes. Demyelination progressed in an orderly fashion such that myelin degeneration began 10 to 20 days after the commencement of myelination in any of the given nerve fiber tracts. This suggests that there are no significant differences in the metabolism of galactocerebroside in the myelin and myelin-forming cells in individual nerve fiber tracts throughout the murine brain. Over-expression of glial fibrillary acidic protein was already present before the initiation of obvious demyelination. In addition to the areas of demyelination, focal clustering of PAS-positive cells were seen in close association with neurons in the basal ganglia and hippocampus in this murine GLD twitcher, whereas in human GLD, PAS-positive cells tended to be limited within the white matter. Understanding of these orderly patterns of neuropathological features is of essential importance for evaluating the results of the forthcoming gene therapy.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00293398
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  • 5
    Electronic Resource
    Electronic Resource
    The role of islet secretory function in the development of diabetes in the GK Wistar rat (1994)
    Springer
    Diabetologia 37 (1994), S. 863-870 
    Details
    ISSN: 1432-0428
    Keywords: Key words Non-insulin-dependent diabetes mellitus ; pancreatic islet ; insulin secretion ; glucose metabolism.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Insulin secretion and glucose metabolism were compared in islets isolated from GK Wistar rats (a non-obese, spontaneous model of non-insulin-dependent diabetes mellitus) and control Wistars aged 8 and 14 weeks. By 8 weeks of age, GK Wistar rats were clearly diabetic as indicated by non-fasting plasma glucose concentrations and impaired glucose tolerance. Islet insulin content was not significantly different to controls at either age. In islets from 14-week-old GK Wistar rats glucose-stimulated insulin release (6–16 mmol/l glucose) was significantly reduced to 25–50 % of controls in static incubations (p 〈 0.001). In perifusion, glucose-stimulated insulin release was reduced by 90 % for first phase (p 〈 0.01) and by 75 % for second phase (p 〈 0.05). The responses to arginine and 2α Ketoisocaproate in islets were similar to those in controls. In contrast, islets isolated from 8-week-old GK Wistar rats exhibited no significant reduction in glucose-stimulated insulin secretion in static incubations. In perifusion, although both first and second phases of glucose-stimulated insulin release were slightly reduced, these were not significantly different to controls. Islets from 8-week-old GK Wistar rats failed however to respond to stimulation by glyceraldehyde. Raising the medium glucose concentration to 16 mmol/l significantly increased rates of glucose utilisation ([3H] H2O production from 5-[3H] glucose) and oxidation ([14C] CO2 production from U-[14C] glucose) in islets isolated from 8-week-old control and GK Wistar rats, respectively. The rates of oxidation were not significantly different at stimulatory glucose concentrations whereas the rates of utilisation were significantly higher in islets from the diabetic animals (p 〈 0.05). Production of [3H] H2O from 2-[3H] glycerol metabolism was increased (p 〈 0.05) at 2 mmol/l glucose but was not significantly different to controls at 16 mmol/l glucose in islets from 8-week-old GK Wistar rats. This data would suggest that abnormalities in islet function are present in 8-week-old diabetic animals although these do not seriously impair glucose-stimulated insulin release from isolated islets. This in turn would indicate that a defect in the glucose signalling pathway in beta cells is not a primary cause of the diabetes of GK Wistar rats and that deterioration of the secretory response is the consequence of some factor associated with the diabetic condition. [Diabetologia (1994) 37: 863–870]
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00400940
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  • 6
    Electronic Resource
    Electronic Resource
    The role of islet secretory function in the development of diabetes in the GK Wistar rat (1994)
    Springer
    Diabetologia 37 (1994), S. 863-870 
    Details
    ISSN: 1432-0428
    Keywords: Non-insulin-dependent diabetes mellitus ; pancreatic islet ; insulin secretion ; glucose metabolism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Insulin secretion and glucose metabolism were compared in islets isolated from GK Wistar rats (a non-obese, spontaneous model of non-insulin-dependent diabetes mellitus) and control Wistars aged 8 and 14 weeks. By 8 weeks of age, GK Wistar rats were clearly diabetic as indicated by non-fasting plasma glucose concentrations and impaired glucose tolerance. Islet insulin content was not significantly different to controls at either age. In islets from 14-week-old GK Wistar rats glucose-stimulated insulin release (6–16 mmol/l glucose) was significantly reduced to 25–50% of controls in static incubations (p〈0.001). In perifusion, glucose-stimulated insulin release was reduced by 90% for first phase (p〈0.01) and by 75% for second phase (p〈0.05). The responses to arginine and 2α Ketoisocaproate in islets were similar to those in controls. In contrast, islets isolated from 8-week-old GK Wistar rats exhibited no significant reduction in glucose-stimulated insulin secretion in static incubations. In perifusion, although both first and second phases of glucose-stimulated insulin release were slightly reduced, these were not significantly different to controls. Islets from 8-week-old GK Wistar rats failed however to respond to stimulation by glyceraldehyde. Raising the medium glucose concentration to 16 mmol/l significantly increased rates of glucose utilisation ([3H] H2O production from 5-[3H] glucose) and oxidation ([14C] CO2 production from U-[14C] glucose) in islets isolated from 8-week-old control and GK Wistar rats, respectively. The rates of oxidation were not significantly different at stimulatory glucose concentrations whereas the rates of utilisation were significantly higher in islets from the diabetic animals (p〈0.05). Production of [3H] H2O from 2-[3H] glycerol metabolism was increased (p〈0.05) at 2 mmol/l glucose but was not significantly different to controls at 16 mmol/l glucose in islets from 8-week-old GK Wistar rats. This data would suggest that abnormalities in islet function are present in 8-week-old diabetic animals although these do not seriously impair glucose-stimulated insulin release from isolated islets. This in turn would indicate that a defect in the glucose signalling pathway in beta cells is not a primary cause of the diabetes of GK Wistar rats and that deterioration of the secretory response is the consequence of some factor associated with the diabetic condition.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00400940
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  • 7
    Electronic Resource
    Electronic Resource
    Spin alignment of43Sc produced in the fragmentation of 500 MeV/u46Ti (1994)
    Springer
    The European physical journal 350 (1994), S. 215-219 
    Details
    ISSN: 1434-601X
    Keywords: 25.70.Np ; 23.20.En ; 21.60.-n
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract In the fragmentation of 500 MeV/u46Ti the spin alignment of43mSc (I=19/2−, T1/2=473 ns) fragments was observed by the Time Dependent Perturbed Angular Distribution (TDPAD) method. The measurement was performed for different cuts in the longitudinal momentum distribution. A positive spin alignment of about 35% was observed in the center and a negative alignment of about 15% in the wing of the distribution. For the different cuts the relative production of the 19/2− state was measured. In the wing of the distribution the isomeric ratio N(19/2−)/total43Sc is about 15 times larger than in the center. The results of this pilot experiment are discussed in the frame of a shell-model.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01289585
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  • 8
    Electronic Resource
    Electronic Resource
    Effects of verapamil on myocardial stunning in xanthineoxidase deficient hearts: Pre-treatment vs. post-ischemic treatment (1994)
    Springer
    Basic research in cardiology 89 (1994), S. 16-28 
    Details
    ISSN: 1435-1803
    Keywords: ATP ; myocardial stunning ; rabbit ; verapamil ; xanthine oxidase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The role of free radicals and the protective action of calcium antagonists have been established in myocardial stunning in canine hearts, which contain a considerable level of xanthine oxidase, a free radical producing enzyme. However, myocardial stunning in hearts which lack xanthine oxidase and its modification by calcium antagonistsin vivo remain uncharacterized. The present study examined this issue using open-chest anesthetized rabbits. Myocardial stunning was induced by a 10-min coronary occlusion and reperfusion. Regional systolic thickening fraction (TF) was determined using an epicardial Doppler sensor, together with other hemodynamic parameters. In untreated control rabbits, recovery of TF from the 10 min transient ischemia was 43±3% of the baseline at 30 min after reperfusion. Administration of verapamil (200 μg/kg bolus plus 40 μg/kg/min), which was started before the onset of ischemia and continued until 20 min after reperfusion, significantly improved the recovery of TF to 74±6% (p〈0.05). A similar improvement in post-ischemic contractile function (TF=77±10%) was observed when verapamil was injected at the same rate, but the infusion was discontinued 1 min after the coronary occlusion. Myocardial ATP depletion after the 10 min ischemia was significantly less in the verapamil-pretreated rabbits compared with untreated controls (10.1±1.0 vs 6.2±0.7 μmol/g dry wt., p〈0.05). The difference in TF between the rabbits with and without verapamil treatment could not be explained by afterload reduction. When verapamil (100 μg/kg bolus plus 20 μg/kg/min) was given during the reperfusion period alone, TF recovery was poorer (TF=22±8%) than the control value. Thus, it was concluded that verapamil attenuates myocardial stunning in the hearts with trace levels of xanthine oxidase, and that the beneficial effect is achieved only by pretreatment, not by post-ischemic treatment with verapamil.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00788674
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  • 9
    Electronic Resource
    Electronic Resource
    Microstructure characterization of sol-gel derived PZT films (1994)
    Springer
    Journal of materials science 29 (1994), S. 436-441 
    Details
    ISSN: 1573-4803
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract The crystallization of sol-gel derived amorphous PZT films deposited on a MgO single-crystal substrate and a SiO2 glass substrate was examined. The pyrochlore crystallites, 5 nm in size, were homogeneously nucleated in the amorphous films at 350 °C. The nucleation temperature of pyrochlore did not depend on the type of substrate. Fine pyrochlore grains were stable even during annealing at high temperatures up to 600 °C. The perovskite formation temperature was dependent on the substrate, and was about 550 °C on the MgO single-crystal substrate and about 750 °C on the SiO2 glass substrate. The perovskite was heterogeneously nucleated preferentially at the substrate-film interface. Perovskite nucleation was more difficult at the SiO2 glass-film interface than at the MgO single crystal-film interface. The ease of nucleation reflected the perovskite formation temperature. Perovskite crystals grew fairly rapidly, once they were nucleated in the films. In the multiple-coated films, the interface between successive layers of PZT films was a favourable nucleation site of perovskite, and the columnar perovskite grains passing through the interface were often developed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01162503
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  • 10
    Electronic Resource
    Electronic Resource
    Molecular genetics of Tay-Sachs disease in Japan (1994)
    Springer
    Journal of inherited metabolic disease 17 (1994), S. 593-600 
    Details
    ISSN: 1573-2665
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00711597
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