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  • 2000-2004  (5)
  • 1995-1999  (2)
  • 1990-1994
  • 1880-1889
  • 2000  (5)
  • 1996  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Gonococcal lipooligosaccharide is a ligand for the asialoglycoprotein receptor on human sperm (2000)
    Oxford, UK : Blackwell Science Ltd
    Molecular microbiology 36 (2000), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: In the present study, we show that Neisseria gonorrhoeae lipooligosaccharide (LOS) can bind to the asialoglycoprotein receptor (ASGP-R) on human sperm. This work demonstrates the presence of ASGP-R on human sperm. Binding of purified ASGP-R ligand decreased in the presence of gonococci. Binding of purified iodinated gonococcal LOS identified a protein of molecular weight corresponding to that of human ASGP-R. The presence of excess unlabelled LOS blocked binding of iodinated gonococcal LOS. Binding of wild-type gonococcal LOS to sperm was higher than that of mutant LOS lacking the galactose ligand for ASGP-R. These data suggest that the ASGP-R on human sperm cells recognizes and binds wild-type gonococcal LOS. This interaction may contribute to the transmission of gonorrhea from infected males to their sexual partners.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2958.2000.01938.x
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  • 2
    Electronic Resource
    Electronic Resource
    A first estimate of organic carbon storage in Holocene lake sediments in Alberta, Canada (2000)
    Springer
    Journal of paleolimnology 24 (2000), S. 395-400 
    Details
    ISSN: 1573-0417
    Keywords: carbon storage ; lake sediment ; Holocene ; Canada ; climate change ; organic matter
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences
    Notes: Abstract This paper reports a first estimate of the Holocene lake sediment carbon pool in Alberta, Canada. The organic matter content of lake sediment does not appear to depend strongly on lake size or other limnological parameters, allowing a simple first estimate in which we assume all Alberta lake sediment to have the same organic matter content. Alberta lake sediments sequester about 15 g C m-2 yr-1, for a provincial total of 0.23 Tg C yr-1, or 2.3 Pg C over the Holocene. Alberta lakes may represent as much as 1/1700 of total global, annual permanent carbon sequestration.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008103605817
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Particle-mediated gene transfer to rat neurons in primary culture (2000)
    Springer
    Pflügers Archiv 439 (2000), S. 730-738 
    Details
    ISSN: 1432-2013
    Keywords: Dorsal root ganglion neurons Gene gun Green fluorescent protein Hippocampal neurons Particle-mediated gene transfer Primary tissue culture Transfection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. Gene transfer into neuronal cells provides an important approach to study their function. Particle-mediated gene delivery was used to transfect rat dorsal root ganglion (DRG) and hippocampal neurons in primary culture with the genes for the enhanced blue and green fluorescent proteins (EBFP and EGFP) under control of the cytomegalovirus promoter. Quantitative analysis of marker protein fluorescence detected expression at 3 h that continued to increase for 48 h. For DRG neurons the optimal expression efficiency of 8±2% was obtained 24 h following transfection. In contrast, approximately 2±1% of hippocampal neurons in culture expressed EGFP at 3 h which subsequently declined. Co-transfection of DRG cultures with two plasmids produced reliable expression of both genes. Transfected DRG neurons exhibited normal electrophysiological properties, and resting and stimulated intracellular Ca2+ concentrations were unchanged. After transfection, 44% of hippocampal neurons remained in functional synaptic networks as indicated by glutamatergic Ca2+ spiking activity. Particle-mediated gene delivery provided a straightforward, reproducible and efficient method for transfection of neurons in primary culture. Transfected cells were easily identified by EGFP fluorescence, enabling subsequent physiological analysis. Biolistic particle bombardment was well tolerated by peripheral neurons, although caution was required when this method was applied to CNS cultures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004249900240
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Minimal Presentations and Efficiency of Semigroups (2000)
    Springer
    Semigroup forum 60 (2000), S. 231-242 
    Details
    ISSN: 1432-2137
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: S is said to be efficient if it can be defined by a presentation (A | R) with |R| -|A|=rank(H 2(S)). In this paper we demonstrate certain infinite classes of both efficient and inefficient semigroups. Thus, finite abelian groups, dihedral groups D 2 n with n even, and finite rectangular bands are efficient semigroups. By way of contrast we show that finite zero semigroups and free semilattices are never efficient. These results are compared with some well-known results on the efficiency of groups.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002339910016
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Microbial Biomass and Community Structure in a Sequence of Soils with Increasing Fertility and Changing Land Use (2000)
    Springer
    Microbial ecology 40 (2000), S. 223-237 
    Details
    ISSN: 1432-184X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The microbial biomass and community structure of eight Chinese red soils with different fertility and land use history was investigated. Two community based microbiological measurements, namely, community level physiological profiling (CLPP) using Biolog sole C source utilization tests and phospholipid fatty acid (PLFA) profiles, were used to investigate the microbial ecology of these soils and to determine how land use alters microbial community structure. Microbial biomass-C and total PLFAs were closely correlated to organic carbon and total nitrogen, indicating that these soil microbial measures are potentially good indices of soil fertility in these highly weathered soils. Metabolic quotients and C source utilization were not correlated with organic carbon or microbial biomass. Multivariate analysis of sole carbon source utilization patterns and PLFAs demonstrated that land use history and plant cover type had a significant impact on microbial community structure. PLFAs showed these differences more than CLPP methods. Consequently, PLFA analysis was a better method for assessing broad-spectrum community differences and at the same time attempting to correlate changes with soil fertility. Soils from tea orchards were particularly distinctive in their CLPP. A modified CLPP method, using absorbance readings at 405 nm and different culture media at pH values of 4.7 and 7.0, showed that the discrimination obtained can be influenced by the culture conditions. This method was used to show that the distinctive microbial community structure in tea orchard soils was not, however, due to differences in pH alone.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002480000053
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    Critical behavior of liquid4He at negative pressures (1996)
    Springer
    Journal of low temperature physics 105 (1996), S. 13-36 
    Details
    ISSN: 1573-7357
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract We examine the equation of state of liquid4He at negative pressures close to the spinodal density ϱs where the hydrodynamic speed of sound vanishes. The non-analytic behavior of the equation of state and the speed of sound in the vicinity of the spinodal density are calculated in two and in three dimensions; we find for the speed of sound the non-analytic behavior mc s 2 ∼ (ϱ — ϱs)2/5 in three dimensions and mc s 2 ∼[(ϱ-ϱs)/¦ln(ϱ-ϱs)¦]1/2 in two dimensions. We then examine the low density regime numerically, using a semi-analytic microscopic theory. It is found that non-analytic exponents are visible only in a negligible density regime around the spinodal point. Estimates for the spinodal densities, and the range of critical fluctuations are provided.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00754626
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  • 7
    Electronic Resource
    Electronic Resource
    Inheritance of neurotoxin (ODAP) content, flower and seed coat colour in grass pea (Lathyrus sativus L.) (1996)
    Springer
    Euphytica 91 (1996), S. 195-203 
    Details
    ISSN: 1573-5060
    Keywords: flower colour ; grass pea ; inheritance ; Lathyrus sativus ; neurotoxin (ODAP or BOAA)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A strong epidemiological association is known to exist between the consumption of grass pea and lathyrism. A neurotoxin, β-N-Oxalyl-L-α, β-diaminopropanoic acid (ODAP) has been identified as the causative principle. This study was undertaken to investigate the mode of inheritance of the neurotoxin ODAP, flower and seed coat colour in grass pea. Five grass pea lines with low to high ODAP concentration were inter-crossed in all possible combinations to study the inheritance of the neurotoxin. Parents, F1 and F2 progenies were evaluated under field condition and ODAP analyzed by an ortho-phthalaldehyde spectrophotometric method. Many of the progenies of low x low ODAP crosses were found to be low in ODAP concentration indicating the low ODAP lines shared some genes in common for seed ODAP content. The F1 progenies of the low ODAP x high ODAP crosses were intermediate in ODAP concentration and the F2 progenies segregated covering the entire parental range. This continuous variation, together with very close to normal distribution of the F2 population both of low x low and low x high ODAP crosses indicated that ODAP content was quantitatively inherited. Reciprocal crosses, in some cases, produced different results indicating a maternal effect on ODAP concentration. Blue and white flower coloured lines of grass pea were inter-crossed to study the inheritance of flower colour. Blue flower colour was dominant over the white. The F2 progenies segregated in a 13:3 ratio indicating involvement of two genes with inhibiting gene interactions. The gene symbol LB for blue flower colour and LW for white flower colour is proposed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00021070
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    Paper (German National Licenses)
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