ISSN:
1617-4623
Keywords:
5-Azacytidine
;
Fission yeast
;
DNA repair
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract We have recently demonstrated thatSchizosaccharomyces pombe cells treated with the nucleoside analogue 5-azacytidine (5-azaC) require previously characterised G2 checkpoint mechanisms for survival. Here we present a survey of known DNA repair mutations which defines those genes required for survival in the presence of 5-azaC. Using a combination of single-mutant and epistasis analyses we find that the excision, mismatch and recombinational repair pathways are all required in some degree for the repair of 5-azaC-mediated DNA damage. There are distinct differences in the epistatic interactions of several of the repair mutations with respect to 5-azaC-mediated DNA damage relative to UV-mediated DNA damage.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02172377
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