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  • 1
    Electronic Resource
    Electronic Resource
    Congenital extension contracture of metacarpophalangeal joints (1997)
    Springer
    Archives of orthopaedic and trauma surgery 116 (1997), S. 426-428 
    Details
    ISSN: 1434-3916
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract We report a case of congenital extension contracture of the fifth metacarpophalangeal joints in a 15-year-old boy who had no associated anomalies and was successfully treated by surgery. Congenital extension contracture of bilateral metacarpophalangeal joints has not been reported previously, and the entity can be considered to be a new subgroup of distal arthrogryposis with congenital distal limb contracture.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004020050154
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Congenital extension contracture of metacarpophalangeal joints (1997)
    Springer
    Archives of orthopaedic and trauma surgery 116 (1997), S. 426-428 
    Details
    ISSN: 1434-3916
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract We report a case of congenital extension contracture of the fifth metacarpophalangeal joints in a 15-year-old boy who had no associated anomalies and was successfully treated by surgery. Congenital extension contracture of bilateral metacarpophalangeal joints has not been reported previously, and the entity can be considered to be a new subgroup of distal arthrogryposis with congenital distal limb contracture.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00434006
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Cryopreservation of zygotic embryos of a Japanese terrestrial orchid (Bletilla striata) by vitrification (1997)
    Springer
    Plant cell reports 16 (1997), S. 754-757 
    Details
    ISSN: 1432-203X
    Keywords: Key wordsBletilla striata ; Cryopreservation ; Embryo ; Orchid ; Vitrification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The seeds of a Japanese terrestrial orchid (Bletilla striata Rchb.f.) were germinated and cultured on solidified new Dogashima (ND) medium for 10 days. These embryos were then precultured on ND medium supplemented with 0.3 m sucrose for 3 days at 25°C in continuous dark. The embryos were then overlaid with a mixture of 2 m glycerol and 0.4 m sucrose for 15 min at 25°C and finally dehydrated with highly concentrated vitrification solution (PVS2) for 3 h at 0°C prior to immersion into liquid nitrogen for 30 min. After rapid warming, the embryos were washed with liquid ND medium supplemented with 1.2 m sucrose for 20 min and then plated on ND medium. Successfully vitrified and warmed embryos developed into normal plantlets. The rate of plant regeneration amounted to about 60%. This vitrification method appears to be a promising technique for cryopreservation of orchids.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050314
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Cryopreservation of in vitro-grown shoot tips of taro (Colocasia esculenta (L.) Schott) by vitrification. 1. Investigation of basic conditions of the vitrification procedure (1997)
    Springer
    Plant cell reports 16 (1997), S. 594-599 
    Details
    ISSN: 1432-203X
    Keywords: Key words Shoot tips ; Cryopreservation ; Vitrification ; Taro [Colocasia esculenta (L.) Schott.] ; Tropical crops
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In vitro-grown shoot tips of taro (Colocasia esculenta (L.) Schott.) were successfully cryopreserved by vitrification. Excised shoot tips precultured on solidified MS supplemented with 0.3 M sucrose and maintained under a 16 h phtoperiod at 25°C for 16 h were loaded with a mixture of 2 M glycerol plus 0.4 M sucrose for 20 min at 25°C. The shoot tips were then sufficiently dehydrated with a highly concentrated vitrification solution (PVS2) for 20 min at 25°C prior to immersion into liquid nitrogen. Successfully vitrified and warmed shoot tips resumed growth within 7 days and developed shoots directly without intermediate callus formation. The average rate of shoot recovery amounted to around 80%, and the vitrification protocol appeared to be very promising for the cryopreservation of taro germplasm.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050285
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Cryopreservation of encapsulated shoot primordia induced in horseradish (Armoracia rusticana) hairy root cultures (1997)
    Springer
    Plant cell reports 16 (1997), S. 469-473 
    Details
    ISSN: 1432-203X
    Keywords: Cryopreservation ; Encapsulation-dehydration ; Encapsulation-vitrification ; Hairy roots ; Horseradish shoot primordia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Shoot primordia induced inArmoracia rusticana Gaertn. Mey. et Scherb. (horseradish) hairy root cultures were successfully cryopreserved by two cryogenic procedures. Encapsulated shoot primordia were precultured on solidified Murashige-Skoog medium supplemented with 0.5M sucrose for 1 day and then dehydrated with a highly concentrated vitrification solution (PVS2) for 4 h at 0°C prior to a plunge into liquid nitrogen. The survival rate of encapsulated vitrified primordia amounted to 69%. In a revised encapsulation-dehydration technique, the encapsulated shoot primordia were precultured with a mixture of 0.5M sucrose and 1M or 1.5M glycerol for 1 day to induce dehydration tolerance and then subjected to air-drying prior to a plunge into liquid nitrogen. The survival rate of encapsulated dried primordia was more than 90%, and the revived primordia produced shoots within 2 weeks after plating. A long-term preservation of shoot primordia was also achieved by the technique. Thus, this revised encapsulation-dehydration technique appears promising as a routine method for the cryopreservation of shoot primordia of hairy roots.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01092768
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    Cryopreservation of invitro-grown shoot tips of taro (Colocasia esculenta (L.) Schott) by vitrification. 1. Investigation of basic conditions of the vitrification procedure (1997)
    Springer
    Plant cell reports 16 (1997), S. 594-599 
    Details
    ISSN: 1432-203X
    Keywords: Shoot tips ; Cryopreservation ; Vitrification ; Taro [Colocasia esculenta (L.) Schott.] ; Tropical crops
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Invitro-grown shoot tips of taro (Colocasia esculenta (L.) Schott.) were successfully cryopreserved by vitrification. Excised shoot tips precultured on solidified MS supplemented with 0.3M sucrose and maintained under a 16 h phtoperiod at 25°C for 16 h were loaded with a mixture of 2M glycerol plus 0.4M sucrose for 20 min at 25°C. The shoot tips were then sufficiently dehydrated with a highly concentrated vitrification solution (PVS2) for 20 min at 25°C prior to immersion into liquid nitrogen. Successfully vitrified and warmed shoot tips resumed growth within 7 days and developed shoots directly without intermediate callus formation. The average rate of shoot recovery amounted to around 80%, and the vitrification protocol appeared to be very promising for the cryopreservation of taro germplasm.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01275498
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    Paper (German National Licenses)
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  • 7
    Electronic Resource
    Electronic Resource
    Cryopreservation of encapsulated shoot primordia induced in horseradish (Armoracia rusticana) hairy root cultures (1997)
    Springer
    Plant cell reports 16 (1997), S. 469-473 
    Details
    ISSN: 1432-203X
    Keywords: Key words Cryopreservation ; Encapsulation-dehydration ; Encapsulation-vitrification ; Hairy roots ; Horseradish shoot primordia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Shoot primordia induced in Armoracia rusticana Gaertn. Mey. et Scherb. (horseradish) hairy root cultures were successfully cryopreserved by two cryogenic procedures. Encapsulated shoot primordia were precultured on solidified Murashige-Skoog medium supplemented with 0.5 M sucrose for 1 day and then dehydrated with a highly concentrated vitrification solution (PVS2) for 4 h at 0°C prior to a plunge into liquid nitrogen. The survival rate of encapsulated vitrified primordia amounted to 69%. In a revised encapsulation-dehydration technique, the encapsulated shoot primordia were precultured with a mixture of 0.5 M sucrose and 1 M or 1.5 M glycerol for 1 day to induce dehydration tolerance and then subjected to air-drying prior to a plunge into liquid nitrogen. The survival rate of encapsulated dried primordia was more than 90%, and the revived primordia produced shoots within 2 weeks after plating. A long-term preservation of shoot primordia was also achieved by the technique. Thus, this revised encapsulation-dehydration technique appears promising as a routine method for the cryopreservation of shoot primordia of hairy roots.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01092768
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    Paper (German National Licenses)
    Fulltext
  • 8
    Electronic Resource
    Electronic Resource
    Preferential degradation of plastid DNA with preservation of mitochondrial DNA in the sperm cells ofPelargonium zonale during pollen development (1997)
    Springer
    Protoplasma 197 (1997), S. 217-229 
    Details
    ISSN: 1615-6102
    Keywords: Biparental inheritance ; Fluorescence microscopy ; Immunoelectron microscopy ; Maternal inheritance ; Pelargonium zonale ; Pollen grains
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In the present study, we studied changes in organellar DNA in the sperm cells of maturing pollen ofPelargonium zonale, a plant typical to exhibit biparental inheritance, by fluorescence microscopy after staining with 4′,6-diamidino-2-phenylindole (DAPI) and by immunogold electron microscopy using anti-DNA antibody. Fluorescence intensities of DAPI-stained plastid nuclei in generative and sperm cells at various developmental stages were quantified with a video-intensified microscope photon counting system (VIMPCS). Results indicated that the amount of DNA per plastid in generative cells increased gradually during pollen development and reached a maximum value (about 70 T per plastid; 1 T represents the amount of DNA in a particle of T4 phage) in young sperm cells at 5 days before flowering. However, the DNA content of plastids was subsequently reduced to about 20% of the maximum value on the day of flowering. Moreover, the DNA content of the plastid further decreased to 4% of the maximum value when pollen grains were cultured for 6 h in germination medium. In contrast, the amount of DNA per mitochondrion did not decrease significantly around the flowering day. Similar results were also obtained by immunogold electron microscopy using anti-DNA antibody. The density of gold particles on plastids decreased during pollen maturation whereas labelling density on mitochondria remained relatively constant. The number of plastids and mitochondria per generative cell or per pair of sperm cells did not change significantly, indicating that the segregation of DNA by plastid division was not responsible for the decrease in the amount of DNA per plastid. These results indicate that the plastid DNA is preferentially degraded, but the mitochondrial DNA is preserved, in the sperm cells ofP. zonale. While the plastid DNA of the sperm cells decreased before fertilization, it was also suggested that the low DNA contents that remain in the plastids of the sperm cells are enough to account for the biparental inheritance of plastids inP. zonale.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01288031
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    Paper (German National Licenses)
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