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  • 2000-2004  (1)
  • 1995-1999  (3)
  • 2003  (1)
  • 1999  (3)
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  • 2000-2004  (1)
  • 1995-1999  (3)
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  • 1
    Electronic Resource
    Electronic Resource
    Analysis of sequential immunoglobulin E-binding epitope of Japanese cedar pollen allergen (Cry j 2) in humans, monkeys and mice (2003)
    Oxford, UK : Blackwell Science Ltd
    Clinical & experimental allergy 33 (2003), S. 0 
    Details
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background Japanese cedar (Cryptomeria japonica; CJ) pollinosis has been reported to occur naturally in Japanese monkeys (Macaca fuscata) as well as in humans. Most human patients and monkeys with pollinosis have specific IgE for Cry j 2, a major allergen of CJ pollen.Objective The main purpose of this study was to identify IgE B cell epitopes of Cry j 2 using a synthetic peptide in humans, monkeys and mice.Methods We synthesized 38 overlapping peptides that span the entire length of Cry j 2. We examined the B cell epitopes of Cry j 2 that are recognized by IgE in the sera of human patients and monkeys with pollinosis and immunized mice using synthetic peptides of Cry j 2. We also examined the reaction of Cry j 2-specific mouse monoclonal IgG antibodies to the peptides. Furthermore, we conducted a histamine release assay with leucocytes from a pollinosis patient using human serum albumin (HSA) conjugated with the peptides as a B cell epitope.Results We found that 16 of the 20 pollinosis patients who had specific IgE to Cry j 2 also exhibited IgE reaction with some Cry j 2 peptides. Of these 16 patients, 10 exhibited IgE reaction with Cry j 2 peptide no. 13 (121GQCKWVNGREICNDRDRPTA140). Five of the seven monkeys with CJ pollinosis exhibited a reaction with peptide no. 13. Furthermore, IgE in mice immunized with Cry j 2 and two mouse monoclonal IgG antibodies reacted with peptide no. 13. Peptide no. 13-conjugated HSA showed the release of histamine from basophils. Furthermore, to determine the minimum epitope in peptide no. 13, we conducted an enzyme-linked immunosorbent assay inhibition test. The core of the epitope in humans, monkeys and mice was 124KWVNGREI131.Conclusion We found that 124KWVNGREI131 is an important B cell epitope recognized by IgE in humans, monkeys and mice.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2222.2003.01579.x
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  • 2
    Electronic Resource
    Electronic Resource
    ISO Far-Infrared Deep Survey of the Lockman Hole (1999)
    Springer
    Astrophysics and space science 266 (1999), S. 313-318 
    Details
    ISSN: 1572-946X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1002675831744
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Genetic diversity of HIV-1 group M from Cameroon and Republic of Congo (1999)
    Springer
    Archives of virology 144 (1999), S. 2291-2311 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary.  We analyzed 57 HIV-1 isolates from Cameroon and the Republic of Congo, with respect to the env C2V3 and/or the pol integrase regions. The results indicated that the topology of the pol tree correlated well with that of the env tree for four clusters of subtype D, F G and H, suggesting that these trees reflect the true evolution of the overall genome structures of these subtypes. However, of 22 Cameroonian isolates that were classified as subtype A based on env, 20 of them diverged in their pol sequence into two lineages that were completely different from the prototypical subtype A, tentatively designated as subtypes A1 and A2. The subtype A1 isolates (6 out of 22) were related in their env C2V3 regions with prototypical subtype A strain, but in their pol regions, they formed an independent cluster that diverged from known HIV-1 subtypes so far reported (except for subtypes I and J). The subtype A2 isolates (14 out of 22), which represent the major epidemic type of HIV-1 in Cameroon, clustered distinctly in both the env and pol trees with the recently described A/G mosaic strains from Nigeria and Djibouti. These two lineages were not spreading in the neighboring Republic of Congo.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s007050050645
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Encapsulation of nitrophenol into AIPO4-5: Effect of isomers on optical second harmonic generations (1999)
    Springer
    Journal of materials science 34 (1999), S. 5509-5512 
    Details
    ISSN: 1573-4803
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract Three isomers of nitrophenol molecules were loaded in one-dimensional channels of AlPO4-5 by vapor-phase diffusion process. The resulting materials (o-NP/,m-NP/ and p-NP/AlPO4-5) have been characterized by thermoanalytic and spectroscopic methods, and their nonlinear optical properties have been investigated in powder form. From the results, these materials were found to exhibit second harmonic generation (SHG). The order of SHG intensity was m-NP/AlPO4-5 〈 p-NP/AlPO4-5 〈 o-NP/AlPO4-5, and SHG intensity of o-NP/AlPO4-5 remarkably depended on amount of loadings.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1004764528890
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    Paper (German National Licenses)
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