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  • 1
    Electronic Resource
    Electronic Resource
    Long-term follow-up of prednisolone and cyclophosphamide therapy in IgA nephropathy (1999)
    Melbourne, Australia : Blackwell Science Asia Pty. Ltd.
    Nephrology 5 (1999), S. 0 
    Details
    ISSN: 1440-1797
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1440-1797.1999.00099.x
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  • 2
    Electronic Resource
    Electronic Resource
    Forensic toxicological implication of acute fatal poisoning cases due to benfuracarb ingestion (1999)
    Springer
    International journal of legal medicine 112 (1999), S. 268-270 
    Details
    ISSN: 1437-1596
    Keywords: Key words Poisoning ; Benfuracarb ; Carbofuran ; Human ; Blood ; Urine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Law
    Notes: Abstract We describe here three cases involving acute fatalities due to benfuracarb ingestion and the forensic toxicological implications. Benfuracarb, a carbamate insecticide and its main metabolite carbofuran, were detected using thin layer chromatography (TLC) and gas chromatography/mass spectrophotometry (GC/MS) after extraction with ethyl acetate and then quantified using gas chromatography (GC) equipped with NPD. The blood levels of benfuracarb and carbofuran were in the range of 0.30∼2.32 μg/ml and 1.45∼1.47 μg/ml, respectively. Benfuracarb was not detected in urine, but carbofuran was detected in the range of 0.53∼2.66 μg/ml.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004140050247
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  • 3
    Electronic Resource
    Electronic Resource
    Antitumor activity of delocalized lipophilic cation, MKT-077 in human carcinomas obtained from fresh surgical specimens (1999)
    Springer
    International journal of clinical oncology 4 (1999), S. 65-68 
    Details
    ISSN: 1437-7772
    Keywords: Key words MKT-077 ; Human gastric cancer ; Chemosensitivity ; MTT assay ; DLC
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Background. MKT-077, a delocalized lipophilic cation, accumulates in mitochondria according to the negative potential inside the mitochondria and exerts cytotoxicity. We investigated the antitumor activity and spectrum of MKT-077 in fresh surgical specimens. Methods. Single cell suspensions of fresh surgical specimens obtained from patients with gastric, colon, and hepatocellular carcinoma were treated with MKT-077 at a cutoff concentration of 30 μg/ml. Cell viability was detected by 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyl-2H tetrazolium bromide (MTT) assay. Results. When the cutoff concentration was 30 μg/ml, the efficacy rates (sensitive cases/evaluated cases in percent) of MKT-077 were 52.6% for gastric cancer, 77.8% for colon cancer, and 35.7% for hepatocellular carcinoma, resulting in an overall efficacy rate of 52.4% (22/42). This was higher than the rates for other agents, including mitomycin C, doxorubicin, 5-fluorouracil, and cisplatin. The antitumor spectrum of MKT-077 was correlated with that of doxorubicin, while the antitumor spectra of mitomycin C, 5-fluorouracil, and cisplatin were completely independent of that of MKT-077. Conclusion. Since MKT-077 seemed to have a different antitumor spectrum from that of the conventionally available agents, further investigation should be conducted, including studies of the combined effect of MKT-077 and other antitumor agents.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s101470050028
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  • 4
    Electronic Resource
    Electronic Resource
    Pyrimidine nucleoside phosphorylase and dihydropyrimidine dehydrogenase indicate chemosensitivity of human colon cancer specimens to doxifluridine and 5-fluorouracil, respectively (1999)
    Springer
    Journal of infection and chemotherapy 5 (1999), S. 144-148 
    Details
    ISSN: 1437-7780
    Keywords: Key words Colon cancer ; Pyrimidine nucleoside phosphorylase (PyNPase) ; Dihydropyrimidine dehydrogenase (DPD) ; 5-Fluorouracil (5-FU) ; Doxifluridine (5′-DFUR)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract We have assessed pyrimidine nucleoside phosphorylase (PyNPase) and dihydropyrimidine dehydrogenase (DPD) activity to compare the chemosensitivity of 5-fluorouracil (5-FU) and doxifluridine (5′-DFUR). Tumor samples were prepared from fresh surgical specimens of 28 patients with advanced colon carcinoma. The activity levels of the two enzymes were assessed as indicators of chemosensitivity to 5′-DFUR and 5-FU. PyNPase activity was analyzed using the HPLC method, and DPD activity was assessed according to the methods of Naguib et al. (1985). A histoculture drug response assay (HDRA) was conducted according to the methods described by Furukawa et al. (1992). The mean and standard deviation of PyNPase activity in the tumor tissue was 110 ± 48.6 μg 5-FU/mg protein/h, which was statistically higher than the corresponding value obtained in normal tissue (60 ± 43.1 μg 5-FU/mg protein/h) (P 〈 0.005). When chemosensitivity to the two drug forms was compared in 16 samples obtained from 16 cases with colon cancer, 1 specimen was sensitive to both drug forms, 3 specimens were exclusively sensitive to 5′-DFUR, 5 specimens were exclusively sensitive to 5-FU, and the other 7 specimens were insensitive to both drugs, without significance. High PyNPase activity was associated with a high chemosensitivity to 5′-DFUR, and high DPD activity correlated with a low chemosensitivity to 5-FU. However, the converse relationship was not found. We suggest that the activity of PyNPase and DPD represents a reliable indicator for the chemosensitivity of colon cancer to 5′-DFUR and 5-FU, respectively.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s101560050024
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  • 5
    Electronic Resource
    Electronic Resource
    Pt L3-edge XANES studies about the hydrogen adsorption on small Pt particles (1999)
    Chester : International Union of Crystallography (IUCr)
    Journal of synchrotron radiation 6 (1999), S. 439-441 
    Details
    ISSN: 1600-5775
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Geosciences , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1107/S0909049598017774
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  • 6
    Electronic Resource
    Electronic Resource
    Alterations of Calcium/Calmodulin-Dependent Protein Kinase II Activity in Ischaemia-Induced Neuronal Death and Neuronal Protection Against Ischaemia in the Gerbil Hippocampus (1999)
    Springer
    Acta neurochirurgica 141 (1999), S. 287-294 
    Details
    ISSN: 0942-0940
    Keywords: Keywords: Transient forebrain ischaemia; delayed neuronal death; neuronal protection; calcium/calmodulin-dependent protein kinase II.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary  To clarify the relation between neuronal protection against ischaemia and calcium/calmodulin-dependent protein kinase II (CaM kinase II) activity, we investigated temporal alterations of the kinase activity in the hippocampus after transient forebrain ischaemia under neuroprotective conditions, employing the gerbil bilateral carotid artery occlusion model.  The hippocampal CA1 neuronal density at 2 hours after 5 minutes of forebrain ischaemia was 214.7±25.8 /mm (mean±S.D.), and did not differ from the control significantly; however, it decreased to 11.7±4.2 /mm at 7 days after the ischaemia. The neuronal density at 7 days after the ischaemia was 185.1±18.5 under the hypothermic conditions, 128.7±19.6 with the brief ischaemic pretreatment, 65.0±13.4 with administration of MK-801, and 20.5±4.2 with the repetitive hyperthermic pretreatment, respectively.  The Ca2+/calmodulin-dependent activity of CaM kinase II in the hippocampal cytosolic fraction was decreased to 47.5% of the control value at 2 hours after the ischaemia, when CA1 neuronal death was not observed. In contrast, the activity was 98.8% of the control under the hypothermic conditions, 91.4% with the brief ischaemic pretreatment, 71.2% with administration of MK-801, and 47.9% with the repetitive hyperthermic pretreatment, respectively.  These results indicated that the preservation of the Ca2+/calmodulin-dependent activity of cytosolic CaM kinase II after ischaemia parallelled the neuroprotective effect in the gerbil hippocampus. Thus, it is suggested that the preservation of the activity may be involved in the mechanism of neuronal protection against ischaemia.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s007010050300
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  • 7
    Electronic Resource
    Electronic Resource
    A new assay for the analysis of X-chromosome inactivation based on methylation-specific PCR (1999)
    Springer
    Human genetics 〈Berlin〉 104 (1999), S. 49-55 
    Details
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The pattern of X-chromosome inactivation in females is currently evaluated by assays of differential methylation in the genes between the active and the inactive X chromosomes, with methylation-sensitive enzymes. We report a new assay in the human androgen receptor (HUMARA) locus involving a methylation-specific polymerase chain reaction (M-PCR) technique, independent of the use of restriction enzymes. The assay involves the chemical modification of DNA with sodium bisulfite and subsequent PCR. By using the assay with specific primers for the methylated allele, we obtained an X-inactivation pattern based on the ratio of the maternal inactive X to the paternal inactive X. These patterns were consistent with those obtained by conventional PCR assay at the same locus in 48 female cases. We also obtained another X-inactivation pattern based on the ratio of the maternal active X to the paternal active X by using specific primers for the unmethylated allele. The latter pattern was complementary to the former pattern, and a combination of these patterns produced a reliable X-inactivation pattern. The assay revealed that 12 (11%) of the 105 normal females had non-random inactivation patterns (〉80:20 or 〈20:80). Four patients with an X; autosome translocation showed extremely non-random patterns, and these results were consistent with those obtained by previous molecular/cytogenetic studies. We conclude that M-PCR provides an accurate assay for X-inactivation and that it can be performed on various DNA samples unsuitable for restriction digestion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004390050909
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  • 8
    Electronic Resource
    Electronic Resource
    Histopathological changes in iridocorneal angle of inherited glaucoma in rabbits (1999)
    Springer
    Graefe's archive for clinical and experimental ophthalmology 237 (1999), S. 654-660 
    Details
    ISSN: 1435-702X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  · Background: We examined morphologically the angular region of eyes affected by inherited glaucoma in rabbits genetically developed by crossbreeding in order to investigate the etiologic changes in the iridocorneal angle and to establish whether this strain of rabbit is a suitable animal model of goniodysgenetic glaucoma in humans. · Methods: The angular regions of both normal and glaucomatous eyes from four rabbits having unilateral inherited glaucoma were observed with light and electron microscopy. · Results: In the glaucomatous eyes angular region, the aqueous plexus corresponding to Schlemm’s canal in humans was open and located far peripherally to the peripheral margin of the anterior chamber angle, although the plexus of one glaucomatous eye was poorly developed with a small lumen. In the angular meshwork, which corresponds to the trabecular meshwork in humans, a thick abnormal tissue with round cells embedded in the extracellular matrix was located just beneath the plexus. A large amount of extracellular matrix of basal lamina-like material was observed in the thick tissue. In the normal eyes, the angular region consisted of well-developed trabecular sheets with neither a thick tissue nor accumulations of extracellular matrix in the angular meshwork. · Conclusion: The findings observed in the glaucomatous eyes are much the same as those observed in goniodysgenetic glaucoma in humans, suggesting that this strain of inherited glaucoma rabbits is a suitable animal model of goniodysgenetic glaucoma in humans. The present study also supports the hypothesis that the presence of a thick subcanalicular tissue due to maldevelopment of the iridocorneal angle is one of the main causes of this type of glaucoma.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004170050293
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