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  • 2005-2009
  • 1990-1994  (2)
  • Agropyron cristatum  (1)
  • Electrophoresis  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 79 (1990), S. 646-653 
    ISSN: 1432-2242
    Keywords: Triticale ; Interspecific hybridization ; Genome rearrangement ; Biochemical markers ; Electrophoresis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Fifty-two progenies originating from a cross between 8x and 4x triticale were submitted to cytogenetic analyses and to various electrophoretic studies [high-molecular-weight (HMW) glutelins, HMW secalins, γ-secalins, α and ω-gliadins, β-amylases] to identify new genetic structures, more specifically the input of the D genome in a genetic context other than the wheat one. Markers of the rye genome (HMW and ω-secalins) were identified in all of the triticale lines, but they originated either from the 4x or from the 8x parent, or from both. Chromosomes 4A, 1B, and 2R, present in both parents, showed the same banding patterns in all progenies. Chromosomes 1R and 5R, present in both parents, showed heterogeneous labelling. The expression of chromosomes 6A, 1D, and 4D, present in the 8x parent only, was more complex with a possible involvement of a regulatory system. Several hexaploid progenies had introgressed part of the D genome, suggesting that crossing 8x and 4x triticale was a practicable approach for transferring D chromosomes into hexaploid triticale.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; Agropyron cristatum ; Alien addition ; RFLP ; Non-radioactive labelling
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A non-radioactive digoxigenin-labelled DNA method was used successfully to identify RFLP markers in 54 Triticum aestivum cv ‘Chinese Spring’ — Agropyron cristatum (2n=28, genome PPPP) P-genome monosomic addition lines. Southern analysis using a set of 14 DNA probes identifying each homoeologous chromosome arm, combined with two restriction enzymes HindIII and EcoRI, indicated that six A. cristatum chromosomes (1P, 2P, 3P, 4P, 5P and 6P) and five A. cristatum chromosome arms (2PS, 2PL, 5PL, 6PS and 6PL) have been individually added to the wheat genome. The added chromosomes of three lines were Agropyron translocated chromosomes. It was also found that two addition plants possessed an Agropyron-wheat translocation. These results showed that RFLP analysis using the set of assigned wheat probes was a powerful tool in detecting and establishing homoeology of alien A. cristatum chromosomes, or arms, added to wheat, as well as in screening the alien addition material. The creation of the monosomic addition lines should be useful for the transfer of disease-resistance genes from A. cristatum to wheat.
    Type of Medium: Electronic Resource
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