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  • 2000-2004  (4)
  • 1995-1999  (5)
  • 1965-1969  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Quantitative assessment of feline epidermal Langerhans cells (1997)
    Oxford, UK : Blackwell Publishing Ltd
    British journal of dermatology 136 (1997), S. 0 
    Details
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The densities of feline epidermal dendritic cells expressing CD18. MHC class II and CD1a antigens were determined for four anatomical locations in 19 cats of European breed in blind conditions. The densities (±SD) of CD1a+ Langerhans cells in the skin of the abdominal wall (269±68 cells/mm2), the back (363±19), the internal side of the ear (572±30) and the external side of the ear (502±32) were significantly different, with young and old animals displaying less stained cells than adults. No significant differences in the mean densities were found with regard to sex, colour or antibody used.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2133.1997.01822.x
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  • 2
    Electronic Resource
    Electronic Resource
    Chloroplast DNA variation in cultivated and wild Prunus avium L: a comparative study (2003)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 122 (2003), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Chloroplast DNA variation in 96 Prunus avium L. cultivars was assessed and compared with the results of a previous study of cpDNA diversity in 23 wild populations of the species. The polymerase chain reaction-restriction fragment length polymorphism method was used in these studies. Approximately 9% of the chloroplast genome was analyzed, using five universal primer pairs and three restriction enzymes. Ten polymorphic fragments were common to both the wild and sweet cherry; eight polymorphic fragments were found only in the wild cherry. In the cultivars, all mutations were small (5-30 bp) indels. In the wild populations, a point mutation was also detected in addition to indels. The mutational combinations revealed three haplotypes in the cultivars, which are the main haplotypes in the wild cherry populations. Chloroplast DNA diversity in wild cherry is higher (16 haplotypes) than in sweet cherry cultivars (three haplotypes). The probable wild origin of the sweet cherry cultivars in the maternal line, on the basis of haplotypic similarity, was discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1439-0523.2003.00768.x
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  • 3
    Electronic Resource
    Electronic Resource
    Granulomatous ulcerative colitis: a re-appraisal of the mucosal granuloma in the distinction of Crohn's disease from ulcerative colitis (2002)
    Oxford UK : Blackwell Science Ltd
    Histopathology 41 (2002), S. 0 
    Details
    ISSN: 1365-2559
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Granulomatous ulcerative colitis: a re-appraisal of the mucosal granuloma in the distinction of Crohn's disease from ulcerative colitis Aims: To determine whether the presence and location of giant cells or granulomas in relation to crypts distinguishes between ulcerative colitis and Crohn's disease. Methods and results: Twenty-nine large bowel mucosal biopsy specimens showing giant cells and/or granulomas in a background more typical of ulcerative colitis than Crohn's disease were collected between 1986 and 1996. Each was subject to detailed independent analysis by three histopathologists. Follow-up of the cases was by examination of all previous and subsequent gastrointestinal surgical or biopsy material and by scrutiny of the clinical notes by a gastroenterologist. On the basis of the accumulated histological data 10 of these 29 cases were accorded the diagnosis of ulcerative colitis. In nine of these 10 cases the clinical diagnosis, where known, was in keeping with this and all nine contained only crypt-associated giant cells and/or granulomas. The tenth case contained a solitary free-standing granuloma and clinically the patient had perianal disease, suggesting that the true diagnosis was Crohn's disease. Conclusions: Isolated giant cells and well-defined epithelioid granulomas distant from crypts do not, as a rule, occur in ulcerative colitis, and hence their presence in a colonoscopic biopsy showing features of chronic inflammatory bowel disease is a strong pointer towards the diagnosis of Crohn's disease. Crypt-associated giant cells and granulomas can occur in ulcerative colitis and in themselves are unreliable features for the discrimination between Crohn's disease and ulcerative colitis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2559.2002.01416.x
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  • 4
    Electronic Resource
    Electronic Resource
    A thermoresistant strain of feline immunodeficiency virus (FIV) with an altered cytopathic effect and a restricted cell tropism (1998)
    Springer
    Archives of virology 143 (1998), S. 2093-2107 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary.  A thermoresistant strain, designated m41, of feline immunodeficiency virus (FIV) was selected after 31 successive passages of chronically infected IRC4 cells at 41 °C. The wild-type virus (wt) which served as a control was cultivated the same number of times at 37 °C. In Crandell feline kidney cells (CrFK), the replication of m41 was similar at 37 °C and 41 °C, whereas wt multiplied only at 37 °C. Furthermore, m41 was more resistant than the wt strain at temperatures ranging from 37 to 56 °C. Syncytia formation was observed with m41 when the CrFK were incubated at 41 °C whereas neither m41 nor wt produced syncytia at 37 °C. The level of replication of wt and m41 on feline lymphoid primary cells at 37 °C was similar. In contrast to wt, m41 was unable to infect bone marrow macrophages. Since one or several mutations in the envelope (env) gene could be involved in changes of cell fusion properties and of cellular tropism, the nucleotide sequence of the env gene derived from wt and m41 respectively was determined. Ten mutations were found in the env gene of m41, thus leading to 9 amino acid modifications in the envelope glycoproteins. These results suggest that structural modifications of the viral envelope proteins are prerequisites for the replication of a thermoresistant FIV strain at elevated temperature and are correlated with the newly acquired viral phenotype.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s007050050446
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  • 5
    Electronic Resource
    Electronic Resource
    Chloroplast DNA diversity within and among populations of the allotetraploid Prunus spinosa L. (2000)
    Springer
    Theoretical and applied genetics 100 (2000), S. 1304-1310 
    Details
    ISSN: 1432-2242
    Keywords: Key words Prunus spinosa L. ; cpDNA diversity ; PCR-RFLP ; Universal primers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  High chloroplast DNA (cpDNA) diversity was found within and among populations of Prunus spinosa sampled from seven European deciduous forests. A study of 12% of the total chloroplast genome detected 44 mutations, which were distributed over 24 haplotypes; four were common to two or more populations and the rest were unique haplotypes. The most-abundant and widely distributed haplotype was H2 (frequency = 41% approximately). Six of the seven populations were polymorphic. All of the six polymorphic populations had ”private” haplotypes (frequency 〈 5%) in addition to common haplotypes. The UPGMA dendrogram demonstrated a correlation between populations and their geographical locations. The total diversity was high (hT = 0.824) and a major portion of it was within populations (hs = 0.663). The level of population subdivision for unordered alleles was low (GST = 19.5%) and for ordered alleles was lower (NST = 13.6%). No phylogeographic structure could be demonstrated in the present geographical scale. High polymorphism in the cpDNA of P. spinosa has to be considered carefully when planning phylogenetic studies involving this species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051439
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  • 6
    Electronic Resource
    Electronic Resource
    Genetic relationships among species of the genus Diplotaxis (Brassicaceae) using inter-simple sequence repeat markers (2000)
    Springer
    Theoretical and applied genetics 101 (2000), S. 1234-1241 
    Details
    ISSN: 1432-2242
    Keywords: Key words Diplotaxis ; Brassicaceae ; ISSR-PCR ; Genetic relationships ; Molecular taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Inter-simple sequence repeat (ISSR) amplification was evaluated for its applicability as a genetic marker system to establish relationships among ten Diplotaxis species. ISSR amplification generated multiple banding profiles with the 12 primers from all DNA samples, with an average of 41.2 fragments per primer. This average was clearly higher for the 5´ triple-anchored primers than for other primers. The banding profiles were highly repeatable across separate PCR runs. DNA mixing procedures were found to be appropriate strategies to generate banding patterns representative of each species studied. Similarity values were calculated considering 494 ISSR bands, and a dendrogram was constructed based on the similarity matrix. The ten Diplotaxis species were clustered into two major groups. The first group consists of five species, Diplotaxis tenuifolia and Diplotaxis cretacea, and Diplotaxis muralis with their putative parents (D. tenuifolia and Diplotaxis viminea). In the second group three species are clustered that are closely related (Diplotaxis virgata, Diplotaxis catholica and Diplotaxis siettiana), in addition to Diplotaxis harra, and Diplotaxis erucoides, which has lowest similarity values with the rest of the species studied. The two groups defined in the present work may be concordant with the idea suggested by several authors of a biphyletic origin for Diplotaxis. The genetic relationships among the ten Diplotaxis species estimated by the polymorphism of ISSR markers are in agreement with those previously inferred by other morphological, biochemical and molecular data, indicating the reliability of the ISSR approach for this purpose.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051602
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  • 7
    Electronic Resource
    Electronic Resource
    Influence of high and low exchangeable Mg and Ca percentages at different degrees of base saturation on growth and chemical composition of citrus plants (1965)
    Springer
    Plant and soil 22 (1965), S. 65-80 
    Details
    ISSN: 1573-5036
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01377690
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  • 8
    Electronic Resource
    Electronic Resource
    Analysis of radioactive metal ions in environmental and liquid effluent samples (1998)
    Springer
    Journal of radioanalytical and nuclear chemistry 228 (1998), S. 5-13 
    Details
    ISSN: 1588-2780
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract This paper describes the development and validation of analytical procedures for the separation and determination of90Sr,90Y,238Pu,239/240Pu,241Am,241Cm and243/244Cm in liquid effluents and environmental samples. The procedures use supported reagents for extraction chromatography (reversed phase partition chromatography) that enable the separation of the analytes from a large number of other radionuclides present.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02387291
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  • 9
    Electronic Resource
    Electronic Resource
    High-resolution, low-voltage SEM for true surface imaging and analysis (1995)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 353 (1995), S. 378-382 
    Details
    ISSN: 1618-2650
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract A novel design concept for the electron optical column has been implemented in the realization of a new ultra-high performance SEM. A compound magnetic/ electrostatic objective lens is at the heart of the high-performance column: the imaging aberrations of this new lens type decrease with decreasing beam energy. Any beam cross-over between the electron source (Schottky FE-gun) and the sample has been eliminated in order to avoid broadening of the beam energy spread (Boersch effect). A high beam energy is maintained throughout the column regardless of the electron probe energy selected by the operator. This protects the beam against the effect of stray fields and minimizes any loss of beam brigthness due to stochastic electron-electron interactions. The new SEM achieves outstanding resolution, particularly at the low beam energies (3 nm achievable at EPE = 1 keV). The secondary electrons emitted by the sample are detected with very high efficiency by an internal annular detector situated above the final lens. Due to the low imaging aberration level, a high current can easily be focused in a very small probe, thus making the new SEM ideally suited for high-resolution, quantitative X-ray analysis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s0021653530378
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  • 10
    Electronic Resource
    Electronic Resource
    High-resolution, low-voltage SEM for true surface imaging and analysis (1995)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 353 (1995), S. 378-382 
    Details
    ISSN: 1618-2650
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract A novel design concept for the electron optical column has been implemented in the realization of a new ultra-high performance SEM. A compound magnetic/electrostatic objective lens is at the heart of the high-performance column: the imaging aberrations of this new lens type decrease with decreasing beam energy. Any beam cross-over between the electron source (Schottky FE-gun) and the sample has been eliminated in order to avoid broadening of the beam energy spread (Boersch effect). A high beam energy is maintained throughout the column regardless of the electron probe energy selected by the operator. This protects the beam against the effect of stray fields and minimizes any loss of beam brightness due to stochastic electron-electron interactions. The new SEM achieves outstanding resolution, particularly at the low beam energies (3 nm achievable at EPE=1 keV). The secondary electrons emitted by the sample are detected with very high efficiency by an internal annular detector situated above the final lens. Due to the low imaging aberration level, a high current can easily be focused in a very small probe, thus making the new SEM ideally suited for high-resolution, quantitative X-ray analysis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00322073
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