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  • 1
    ISSN: 1432-0533
    Keywords: Key words Aciculin ; Dystrophin ; Binding site ; Normal and dystrophic muscles ; Ultrastructural ¶localization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Aciculin is a novel adherens junction antigen extracted from human uterine smooth muscle that is reported to associate biochemically with dystrophin. We attempted to determine (i) the immunostainability of anti-aciculin antibody for the 6 histochemically normal human muscles and seven muscles from boys with Duchenne muscular dystrophy(DMD) and 11 disease control muscles, (ii) the ultrastructural localization of aciculin in normal skeletal myofibers, (iii) aciculin’s spacial relationship with dystrophin and β-spectrin, and (iv) if the aciculin is ultrastructurally colocalized with dystrophin, the distance from the aciculin epitope to the epitope of the dystrophin N- or C-terminal domain. For this, rabbit anti-aciculin antibody was generated against the synthetic peptide of aciculin fragment D [4]. Immunohistochemical staining showed that the immunostainability of DMD muscles for anti-aciculin antibody was markedly decreased as compared with normal and disease control muscles. Single and double immunogold labeling electron microscopy of 6 histochemically normal human quadriceps femoris muscles revealed that aciculin was present along the inner surface of muscle plasma membrane and that aciculin formed doublets more frequently with dystrophin (23.5 ± 1.8%; group mean ± SE) than with β-spectrin (12.8 ± 1.1%; P 〈 0.01 two tailed t test). Rabbit anti-aciculin antibody frequently formed doublets with monoclonal antibodies against the N- or C-terminal domain of dystrophin at the muscle cell surface. These results suggest that aciculin is associated with dystrophin and may interact with both the N- and C-terminal domains of dystrophin.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Environmental geology 39 (2000), S. 279-291 
    ISSN: 1432-0495
    Keywords: Key words Longwall mining ; Channel morphology ; Landslides ; Aquatic habitat ; Wasatch Plateau
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences
    Notes: Abstract  Short-term geomorphic and hydrologic effects of subsidence induced by longwall mining under Burnout Creek, Utah were evaluated. During the year after longwall mining, 0.3–1.5 m of subsidence was measured near impacted reaches of the mountain stream channel. The major channel changes that occurred in a 700-m reach of Burnout Creek that was subsided from 1992 to 1993 were: (1) increase in lengths of cascades and to a lesser extent glides; (2) increases in pool length, numbers and volumes; (3) increase in median particle diameter of bed sediment in pools; and (4) some constriction in channel geometry. Most of the changes appeared short-lived, with channel recovery approaching pre-mining conditions by 1994. In a 300-m reach of the South Fork that was subsided from 1993 to 1994, only channel constriction was observed, although any impacts on pool morphology may have been confounded by heavy grazing in the riparian reaches during the dry summer of 1994. Similar near-channel sedimentation and loss of pool volume between 1993 and 1994 were noted throughout Burnout Creek and in adjacent, unmined James Creek. Subsidence during the 3-year period had no effect on baseflows or near-channel landslides.
    Type of Medium: Electronic Resource
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