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Notes: Abstract Chromian omphacite which contains up to 4 wt % Cr2O3 has been identified from low-grade metamorphic rocks in Nishisonogi, Kyushu, Japan. It occurs as aggregates, forming a thin horizon ([20 mm thick) in alayered metagabbro within a serpentinite melange zone, together with Cr-free omphacite, actino-lite, epidote and sphene. It may have been formed by the metasomatic introduction of Cr into the metagabbro from the serpentinite rather than by reaction with chromite. The structural formula, based on EPMA analyses, and the optical absorption spectrum of the chromian omphacite show that the Cr is positioned in the octahedral site.

Notes: Measurements of dry weight (wt), carbon (C), nitrogen (N) and calories were made on walleye pollock eggs (0.24 mg, 35.3% C, 8.3% N, and 4.6 kcal g−1 dry wt), larvae (0.16 g, 42.9% C, 11.1% N and 5.1 kcal g−1 dry wt) and juveniles (22.4 g, 47.2% C, 9.0% N and 5.6 kcal g−1 dry wt). For juvenile fish (9–360 g wet wt) the measured values were related to dry weight and Fulton's condition factor index (CFI) by regression models. The CFI was a better predictor of body composition than dry weight. As CFI improved from a minimum starvation level of 0.42 to a maximum of 1.16, body caloric content, percentage C, and the C/N ratio increased (kcal g−1 dry wt = 4.4 CFI + 1.7, percentage carbon = 49.7 CFI0.5, C/N ratio = 5.0 CFI + 0.9), while percentage N and percentage ash decreased (percentage N =−3.5 CFI + 12.1; percentage ash = 9.1 CFI−1.4). The results of this study suggest that seasonal C, N and caloric content of young pollock can be estimated from measurements of Fulton's condition factor index.

Notes: To determine whether a 5-day regimen with rabeprazole, clarithromycin and amoxicillin (RCA) was as effective as a 7-day regimen.〈section xml:id="abs1-2"〉〈title type="main"〉Methods:A total of 139 H. pylori-infected patients were randomized to receive either a 5-day or 7-day course of rabeprazole 10 mg b.d., clarithromycin 400 mg b.d. and amoxicillin 750 mg b.d. Eradication was assessed by CLO test, histology and 13C-urea breath test.〈section xml:id="abs1-3"〉〈title type="main"〉Results:On the intention-to-treat basis, eradication rates were 66% (46 out of 70) and 84% (58 out of 69) for the 5- and 7-day regimens, respectively (P 〈 0.05). Using per protocol analysis, eradication rates were 70% (46 out of 66) and 91% (58 out of 64) for the 5- and 7-day regimens, respectively (P 〈 0.01). Adverse events, which were observed in 14 patients from each group, caused discontinuation of treatment in only two patients, resulting in excellent compliance.〈section xml:id="abs1-4"〉〈title type="main"〉Conclusions:Our 5-day regimen of RCA yielded inferior results, whereas the 7-day regimen achieved an eradication rate exceeding 90% on the per protocol basis. Therefore, treatment regimens of less than 7 days for proton pump inhibitor–clarithromycin–amoxicillin therapies cannot be recommended.

Notes: Summary  Background Cultured epidermal autographs (CEAs) are currently used as a coverage treatment for burn wounds, for disfiguring burn scars involving depigmentation and in restoring the elasticity of the skin. The advantage of CEAs is that epidermal sheets prepared from small skin pieces can be enlarged sufficiently to cover large burn areas. Objectives We examined the correlation between recovery of skin texture, and elastic fibre formation and keratinocyte differentiation (assessed by immunohistochemistry) in CEAs used as replacement skin after tattoo excision in a Japanese patient. Methods The tattooed skin was excised down to the deep dermal layer and then CEA was transplanted onto the patient. The skin textures were evaluated by taking replicas of the skin surface, and histological changes of filaggrin, transglutaminase, involucrin, fibrillin and elastin in the autograft skin were examined by immunohistochemistry. Results The skin texture improved with time after grafting the CEA, and appeared similar to that of normal skin at 39 months. Among keratinocyte differentiation markers, filaggrin recovered to a normal pattern at around 6 months, and transglutaminase did so at 39 months, whereas involucrin expression remained abnormal at 39 months. Fibrillin expression appeared similar to that of normal skin by 39 months, except for sparse candelabra-like structures of short fibres. Elastin expression remained at a low level throughout. Conclusions Our results show that the recovery of skin texture after application of CEAs following tattoo excision is associated with the normalization of epidermal differentiation markers, except involucrin, and with the regeneration of elastic fibres in the dermis.

Notes: Background  Laminin 5 is known to induce the adhesion, spreading and migration of human keratinocytes. In skin wound healing, laminin 5 deposition beneath migrating keratinocytes occurs early and is followed by the formation of hemidesmosomes and then basement membrane.Objectives  To identify factors that regulate the synthesis and secretion of laminin 5 by human keratinocytes during acute wound healing.Methods  Laminin 5 synthesis by human keratinocytes was determined by a specific sandwich enzyme-linked immunosorbent assay. To determine the total amount of laminin 5 synthesized, laminin 5 deposited on culture dishes and inside cells was solubilized by detergent solution and determined separately from conditioned medium, and the total laminin 5 synthesis was calculated. A quantitative polymerase chain reaction method was used to measure the expression levels of laminin 5 genes, LAMA3, LAMB3 and LAMC2, which correspond to the α3, β3 and γ2 chains of laminin 5. We also examined the effects of lysophospholipids, proinflammatory cytokines and growth factors, which are components in acute wound fluids, on laminin 5 synthesis in keratinocytes.Results  Human acute wound fluid at days 1, 2 and 3 stimulated laminin 5 synthesis in cultured human keratinocytes in a concentration-dependent manner, although findings are restricted to one case. Human serum also increased laminin 5 production by human keratinocytes as strongly as the wound fluid did, suggesting that the major active components in acute wound fluid may be derived from those in human serum. Lysophospholipids such as lysophosphatidic acid (LPA), lysophosphatidylcholines (LPCs) and sphingosine-1-phosphate (S1P) increased laminin 5 synthesis in a concentration-dependent manner. Among growth factors, epidermal growth factor, insulin-like growth factor-1, interferon-γ and keratinocyte growth factor increased laminin 5 production in keratinocytes, while platelet-derived growth factor, hepatocyte growth factor and basic fibroblast growth factor were ineffective. Although interleukin-1α had no effect, transforming growth factor (TGF)-α, tumour necrosis factor (TNF)-α and TGF-β1 also stimulated laminin 5 synthesis, and TGF-α and TGF-β1 showed a synergistic effect. Neutralizing antibodies to TGF-α and TGF-β1 markedly inhibited the enhanced laminin 5 synthesis by human serum, suggesting that TGF-α and TGF-β1 are important components to increase laminin 5 in human serum. In line with the increase of laminin 5 synthesis, the expression levels of all three laminin 5 genes were also augmented by TGF-α and TGF-β1.Conclusions  Laminin 5 synthesis in human keratinocytes was augmented by inflammatory cytokines and growth factors such as TGF-α, TGF-β1 and TNF-α, and lysophospholipids such as S1P, LPA and LPCs, which are supposed to be present in acute wound fluid. The increased laminin 5 protein in the wound area presumably enhances wound repair by stimulating adhesion and migration of keratinocytes on the wound bed and by facilitating basement membrane formation at the dermal–epidermal junction.

Notes: Summary The brain of a 69-year-old man exhibited extensive granulomatous inflammation in the walls of arteries in the leptomeninges, associated with amyloid deposition in the media of the involved arteries. The extracranial arteries exhibited neither granulomatous inflammation nor amyloid deposition in their walls.

Notes: Summary Immunoreactive TRH-containing neurons and their synaptic associations were studied electron microscopically in the paraventricular nucleus (PVN) and dorsomedial nucleus (DMH) of the rat hypothalamus. In propylthiouracil (PTU)-treated rats, the immunoreactive cell bodies in the PVN appeared to be activated, showing a hypertrophic perikaryon, well developed Golgi bodies and numerous secretory granules. No such alterations were evident in the TRH neurons in the DMH. These findings suggest that the PVN-TRH neurons are involved in the hypothalamic-hypophysial-thyroid axis. Further, it was shown that unlabeled nerve terminals containing small and large clear vesicles make synaptic contacts with the TRH perikarya in the PVN. Thus it is likely that PVN-TRH neurons are regulated both by thyroid hormones and by other neuronal signals. In the DMH, unlabeled nerve terminals containing small and large clear vesicles, and immunoreactive terminals form synapses with TRH neurons. Thus the DMH-TRH neurons may be under dual neuronal control. It was further noted that in the DMH and PVN, TRH nerve terminals make synaptic contacts with other unlabeled neurons. It is evident that TRH acts as a neurotransmitter or neuromodulator, although the origin of TRH terminals should be elucidated.