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  • 11
    Electronic Resource
    Electronic Resource
    Association between interleukin-18 gene polymorphism 105A/C and asthma (2003)
    Oxford, UK : Blackwell Science Ltd
    Clinical & experimental allergy 33 (2003), S. 0 
    Details
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background IL-18 has been shown to exert anti-allergic or allergy-promoting activities, but the existence of genetic polymorphisms in the coding regions of IL-18 gene has not been demonstrated.Objective The aim of this study was to investigate whether polymorphism is present in the coding regions of the IL-18 gene and, if so, to further analyse the association between polymorphism and asthma in a case–control study.Methods We screened the coding regions of the IL-18 gene for polymorphisms by using PCRsingle-stranded conformation polymorphism and direct sequencing of PCR products, followed by analysis of the association between polymorphism and asthma.Results We identified one polymorphism (105A/C) in the coding regions. The frequency of the 105A allele was significantly higher in asthmatic patients than in controls (P〈0.01; odds ratio (OR)=1.83 (1.37–2.26)). Significant linkage disequilibrium was observed between the 105A/C and −137G/C polymorphisms in the 5′ flanking region of the IL-18 gene (D=0.58, P〈0.0001). However, in asthmatic patients the 105A allele was not associated with either total serum IgE or IL-18 levels.Conclusion The 105A/C polymorphism of the IL-18 gene may be associated with the pathogenesis of asthma.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2222.2003.01739.x
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  • 12
    Electronic Resource
    Electronic Resource
    S-IV-02 
C2C12 Cells Differente in Three Dimensional Culture in Collagen Gel (2004)
    Oxford, UK; Malden, USA : Blackwell Science Inc
    Wound repair and regeneration 12 (2004), S. 0 
    Details
    ISSN: 1524-475X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Aim: The movements of the cell population are different between 2-D culture and 3-D culture. From the observation of morphology of skeletal muscle cell 3-D culture, We expect that skeletal muscle cells differentiation is accelerate in the collagen gel 3-D culture, and the proliferation is suppressed. The purpose of this study is to investigate the difference between 2-D culture and 3-D culture of C2C12 cells. Methods: C2C12 skeletal muscle cells are incubated following three difference conditions for 48 hours, plastic dish 2-D culture, collagen coated dish 2-D culture and collagen gel 3-D culture. The culture medium is Dulbecco's modified Eagle medium containing 10% fetal bovine serum and 1% penicillin/streptomycin. Collagens are removed by collagenase treatment and cells are homogenized. After centrifugation the top clear layer is used for CPK assay and protein development analysis by Western blotting Results: After 48 hour incubation, we observed cell morphology by a phase contract microscope. Cell fusion was observed in collagen gel 3-D culture. The fusion cells have many nucleus in the cytoplasm called synthetium. But in plastic dish 2-D culture and in collagen coated dish 2-D culture synthetiums were not observed and cells were mononuclear and monolayer. Cell prolieration was suppressed in collagen gel 3-D culture. CPK activity was five times activated in collagen gel 3-D culture than in plastic dish 2-D culture. Conclusions: We suggest skeletal muscle cells C2C12 are activate differentiation by collagen gel 3-D culture.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1067-1927.2004.abstractj.x
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  • 13
    Electronic Resource
    Electronic Resource
    Bonding abutments to cast metal post/cores: comparison of pre-treatment effects (2003)
    Oxford, UK : Blackwell Science Ltd
    Journal of oral rehabilitation 30 (2003), S. 0 
    Details
    ISSN: 1365-2842
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: summary  Bond strengths were evaluated for (1) metal primer systems when the metal was contaminated by a dentin conditioner and (2) a dentin adhesive system when dentin was contaminated by metal primers. Disc specimens were cast in a silver–palladium–copper–gold (Ag–Pd–Cu–Au) alloy and dentin specimens were prepared by grinding the labial surface of bovine teeth. Specimens were treated with (1) metal primer alone, (2) dentin conditioner alone, (3) metal primer followed by dentin conditioner and (4) dentin conditioner followed by metal primer. A resin cement was poured into a mould over a restricted bonding area and allowed to set. Metal specimens were shear stressed to failure after thermocycling (4–60 °C; 20 000 cycles). Dentin specimens were stressed in the same manner after 24 h of immersion in 37 °C water. The results were compiled and analysed by anova. Data for dentin specimens treated with dentin conditioner only or with the combination of dentin conditioner and metal primer were not significantly different, statistically. Post-thermocycled groups indicated that bond strengths to the alloy significantly decreased (P 〈 0·05) when the primed metal surface was contaminated with dentin conditioner regardless of the timing of its application.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2842.2003.01016.x
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  • 14
    Electronic Resource
    Electronic Resource
    Multilocular giant epidermal cyst (2004)
    Oxford, UK : Blackwell Science Ltd
    British journal of dermatology 151 (2004), S. 0 
    Details
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2133.2004.06227.x
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  • 15
    Electronic Resource
    Electronic Resource
    Bilateral Bowen's disease (2004)
    Oxford, UK : Blackwell Science Ltd
    British journal of dermatology 151 (2004), S. 0 
    Details
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Multiple Bowen's disease may be difficult to differentiate from bowenoid papulosis because of its clinicopathological resemblance to bowenoid papulosis. We experienced a case of bilaterally and symmetrically developed multiple bowenoid lesions in a 71-year-old man previously diagnosed as having chronic lymphocytic lymphoma (CLL). Based on histological findings and the results of human papillomavirus examinations, we finally diagnosed this case as bilateral Bowen's disease. We speculate that the underlying immunosuppressive state due to CLL may have been associated with onset of the disease. We report the unique clinical picture, the differential diagnosis and the aetiology.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2133.2004.06039.x
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  • 16
    Electronic Resource
    Electronic Resource
    Administration of anti-interleukin 18 antibody fails to inhibit development of dermatitis in atopic dermatitis-model mice NC/Nga (2003)
    Oxford, UK : Blackwell Science Ltd
    British journal of dermatology 149 (2003), S. 0 
    Details
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Summary Background Interleukin (IL)-18 has been shown to activate basophils to produce histamine and IL-4 and to induce naive T cells to differentiate into T-helper (Th) 2 cells. However, when expressed together with IL-12, IL-18 induces Th1 cell development and inhibits IgE synthesis. Previously we reported that serum IL-18 levels were elevated in the sera from atopic dermatitis-model mice NC/Nga, prior to the onset and during the development of dermatitis. Objectives We studied whether neutralization of IL-18 activity might affect dermatitis in NC/Nga mice, to investigate the role of IL-18 on dermatitis. Methods NC/Nga mice were given weekly anti-IL-18 antibody starting at 5 weeks of age to 13 weeks and development of dermatitis, scratching behaviour and serum IgE concentrations were evaluated. Results Continuous injections of anti-IL-18 antibody failed to inhibit the onset and development of dermatitis and IgE elevation. The treatment, rather, tended to lead to an exacerbation of dermatitis and scratching behaviour. In addition, the administration of anti-IL-18 antibody did not ameliorate the responsiveness of lymphocytes to IL-4, which was previously demonstrated as an immunological abnormality in the mouse. Conclusion This study demonstrates that, at least in NC/Nga mice, IL-18, although excessively expressed before the onset of dermatitis, shows antiallergic actions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2133.2003.05406.x
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  • 17
    Electronic Resource
    Electronic Resource
    Oral administration of persimmon leaf extract ameliorates skin symptoms and transepidermal water loss in atopic dermatitis model mice, NC/Nga (2002)
    Oxford, UK : Blackwell Science, Ltd
    British journal of dermatology 146 (2002), S. 0 
    Details
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Summary Background  We have previously shown that persimmon leaf extract and its major flavonoid constituent, astragalin, inhibited histamine release by basophils and that oral administration of these substances prior to the onset into an atopic dermatitis (AD) model mouse, NC/Nga, prevented development of dermatitis.Objectives  This study was designed to assess the clinical therapeutic effect of persimmon leaf extract and astragalin in NC/Nga mice suffering from dermatitis and the dose–response preventive effects of persimmon leaf extract on dermatitis and transepidermal water loss (TEWL).Methods  The efficacy of persimmon leaf extract or astragalin in NC/Nga mice was judged by measurement of skin severity, scratching behaviour, serum IgE levels or TEWL.Results  Oral administration of persimmon leaf extract (250 mg kg−1) or astragalin (1·5 mg kg−1) for 4 weeks into NC/Nga mice with overt dermatitis resulted in a decrease in the severity of the condition. The preventive effect of persimmon leaf extract on the dermatitis was dose-dependent and continuous intake of persimmon leaf extract significantly decreased its onset and development. In addition, TEWL was also suppressed at a persimmon leaf extract dose of 250 mg kg−1. No significant adverse reaction by these substances could be observed.Conclusions  These observations suggest that persimmon leaf extract or the flavonoid astragalin may be alternative substances for the management of AD.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2133.2002.04557.x
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  • 18
    Electronic Resource
    Electronic Resource
    Epidermolysis bullosa acquisita sera react with distinct epitopes on the NC1 and NC2 domains of type VII collagen: study using immunoblotting of domain-specific recombinant proteins and postembedding immunoelectron microscopy (2004)
    Oxford, UK : Blackwell Science Ltd
    British journal of dermatology 150 (2004), S. 0 
    Details
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background  The sera of epidermolysis bullosa acquisita (EBA) react with type VII collagen, a major component of anchoring fibrils, in which the major epitopes have been considered to be present in the N-terminal noncollagenous (NC) 1 domain.Objectives  To determine whether there are also epitopes in the C-terminal NC2 domain, and to determine their ultrastructural localization.Methods  Immunoblotting using recombinant proteins of the NC1 and NC2 domains of type VII collagen, and postembedding immunoelectron microscopy.Results  Twenty of 28 EBA sera tested reacted with the NC1 domain and eight sera reacted with the NC2 domain. The sera that reacted with the NC1 domain showed immunoreactivity within the lamina densa and the sera that reacted with the NC2 domain showed immunoreactivity in the dermis 300–360 nm below the lamina densa.Conclusions  This study clearly identified the presence of epitopes in the NC2 domain, and showed that the epitope in the NC1 domain is present in the lamina densa and that the epitope in the NC2 domain is in the dermis below the lamina densa.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2133.2004.05933.x
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  • 19
    Electronic Resource
    Electronic Resource
    Compound heterozygous group A xeroderma pigmentosum patient with a novel mutation and an inherited reciprocal translocation (2000)
    Oxford, UK : Blackwell Science Ltd
    British journal of dermatology 143 (2000), S. 0 
    Details
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The severity of neurological abnormalities in Japanese group A xeroderma pigmentosum (XP-A) patients correlates with the sites of non-sense mutation in the XP-A gene. We describe a patient who presented with a more severe photosensitivity and neurological abnormality than those in typical Japanese XP-A patients with a splicing mutation in intron 3. The patient was compound heterozygous for the splicing mutation in intron 3, which resulted in formation of a non-sense codon in exon 4, and a novel non-sense mutation at codon 208 in exon 5, a C to T transition creating a stop codon TAG. Although the combination of these mutations might have been thought to cause only mild neurological signs, the longer truncated XP-A proteins than those of typical XP-A patients may have resulted in severe neurological symptoms. This phenomenon may be explained by a translocation of chromosome (1;10)(q25·3;q22·3) inherited from his father.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2133.2000.03611.x
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  • 20
    Electronic Resource
    Electronic Resource
    The La–Ce geochronometer: a new dating method (1982)
    [s.l.] : Nature Publishing Group
    Nature 300 (1982), S. 515-518 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Radioactivity of 138La has been predicted, because of Mat-tauch's rule concerning the stability of isobaric atomic nuclei CffBa-^La-^fCe) with contiguous atomic numbers and the half-life has been measured1'11'25. 138La decays by /3 emission to an excited level of 138Ce which emits 789 keV y ray and ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/300515a0
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