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  • 2000-2004  (4)
  • 1970-1974  (2)
  • 1
    ISSN: 1399-0047
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Notes: Intein homing endonucleases are proteins spliced out from a precursor protein and site-specific enzymes that make double-strand breaks in inteinless alleles. Crystals of intein homing endonuclease II from the hyperthermophilic archaeon Pyrococcus kodakaraensis strain KOD1 (PI-PkoII) have been grown at room temperature using ammonium sulfate as a precipitant. The diffraction pattern of the crystal extends to 3.0 Å resolution at room temperature upon exposure to synchrotron X-rays at KEK-PF, Japan. The crystals have symmetry consistent with space group C2221, with unit-cell parameters a = 107.6, b = 150.5, c = 146.8 Å. A full set of X-ray diffraction data were collected to 3.0 Å Bragg spacing from a native crystal with an overall Rmerge of 4.8% and a completeness of 96.6%.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1399-0047
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Notes: Glutathione-dependent dehydroascorbate reductase (GSH-DHAR) catalyzes the reduction of dehydroascorbate to ascorbate using reduced glutathione as the electron donor. GSH-DHAR from spinach chloroplasts produced in Escherichia coli was crystallized by the hanging-drop vapour-diffusion method. The crystals were monoclinic, space group C2, with unit-cell parameters a = 98.25, b = 39.96, c = 106.86 Å, β = 110.46°. The asymmetric unit contained two molecules, giving a crystal volume per enzyme mass (VM) of 2.06 Å3 Da−1 and a solvent content of 40.3%. A full set of X-ray diffraction data were collected to 2.2 Å Bragg spacing from three native crystals with an overall Rmerge of 6.5% and a completeness of 93.4%.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1072-8368
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Medicine
    Notes: [Auszug] Here we report the crystal structures of human hematopoietic prostaglandin (PG) D synthase bound to glutathione (GSH) and Ca2+ or Mg2+. Using GSH as a cofactor, prostaglandin D synthase catalyzes the isomerization of PGH2 to PGD2, a mediator for allergy response. The enzyme is a homodimer, and Ca2+ ...
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  • 4
    ISSN: 1573-5028
    Keywords: chitinase function ; flower-predominant ; gene expression ; molecular cloning ; monocotyledon ; promoter
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A flower-predominant cDNA for a gene, termed OsChia1;175, was isolated from a cDNA library of rice pistils. Northern blot and RT-PCR analyses revealed that the OsChia1;175 gene is highly expressed in floral organs (pistils, stamens and lodicules at the heading stage) but not or at an extremely low level in vegetative organs. OsChia1;175 encodes a protein that consists of 340 amino acid residues, and the putative mature protein shows 52% to 63% amino acid identity to class I chitinases of rice or other plants. The phylogenetic tree shows that the OsChia1;175 protein is a new type of plant class I chitinase in rice. The expression of OsChia1;175 in vegetative organs is not induced by several chemicals, UV, and wounding. The soluble putative mature OsChia1;175 protein expressed in Escherichia coli exhibited chitinase activity in the assay with colloidal chitin as a substrate. Genomic Southern analysis revealed that the OsChia1;175 gene was organized as a low-copy gene family. The rice genomic library was screened and a genome clone corresponding to OsChia1;175 was isolated. The transcription start sites of the OsChia1;175 gene were mapped by primer extension analysis. The 1.2 kb putative promoter region of the OsChia1;175 gene was fused to the GUS (β-glucuronidase) gene, and this chimeric gene was introduced to rice by Agrobacterium-mediated transformation. The flower-predominant gene expression was identified also in the transgenic rice plants. The high promoter activity was detected in the stigmas, styles, stamens and lodicules in transgenic plants. The possible functions of OsChia1;175 are discussed.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Digestive diseases and sciences 18 (1973), S. 670-678 
    ISSN: 1573-2568
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Cholesterol solubility was determined in model systems of unconjugated and conjugated bile salts and lecithin at physiologic concentrations. Conjugated bile salts had somewhat less dissolving power than unconjugated forms, with taurine conjugates showing less power than glycine conjugates. Lecithin increased the dissolving power of each bile salt species proportionally up to a lecithin-bile salt molar ratio of 1.0, at which point the amount of cholesterol dissolved was triple that in the absence of lecithin. At most physiologic ratios of lecithin-bile salt, however, lecithin only doubles the amount of cholesterol dissolved. Lecithin reduces, but does not eliminate, significant differences in the cholesterol dissolving power of both unconjugated and conjugated bile salt species. Mixtures of unconjugated bile salts show a simple additive effect in the absence of lecithin, but when lecithin is present the dissolving power of deoxycholate predominates over cholate, and of cholate over chenodeoxycholate. Mixtures of conjugated bile salt species produce a simple additive effect on dissolving power in both the presence and the absence of lecithin. Our cholesterol saturation curves for glycodeoxycholate and glycocholate at a total bile salt concentration of 150 mM, which we consider representative of the cholesterol dissolving power of human gallbladder bile, showed less dissolving power at lecithin-bile salt ratios of 0.25 to 0.50 than did the curve of Adminrand and Small; our curves were reasonably similar to those of Hegardt and Dam on the trilinear graph. Our studies show that changes in total bile salt concentration encountered in human gallbladder bile do produce significant shifts in the saturation curve.
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Digestive diseases and sciences 18 (1973), S. 1067-1074 
    ISSN: 1573-2568
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Reflux of pancreatic secretions and bacterial infection have been suggested as important factors in gallstone formation in some instances by introducing into bile phospholipases hydrolyzing lecithin to lysolecithin, mono- and diglycerides, and free fatty acids. Since there is little data on free fatty acids, we studied the effect of sodium oleate, the soap of one of the major fatty acid derivatives of lecithin, on cholesterol solubility in unconjugated bile salt-lecithin model solutions to see if an increase in this component might lead to saturation of bile with cholesterol. In the absence of lecithin, sodium oleate decreased cholesterol solubility in bile salt solutions at concentrations physiologic for bile, although cholesterol solubility was increased by oleate at higher oleate-bile salt ratios. In the presence of lecithin, sodium oleate decreased cholesterol solubility at all concentrations studied. Significant differences in cholesterol solubility were found for all comparable concentrations of sodium cholate and deoxycholate studied, both in the presence and absence of lecithin. Our studies showed that an increase in free fatty acid concentration can increase cholesterol saturation significantly in unconjugated bile salt-lecithin model solutions. Whether or not free fatty acid concentrations in pathologic bile reach levels sufficient to contribute to cholesterol saturation and gallstone formation cannot be determined until more adequate data on the minor lipid composition of bile becomes available.
    Type of Medium: Electronic Resource
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