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  • 1
    Electronic Resource
    Electronic Resource
    Crystallization and preliminary X-ray crystallographic analysis of archaeal O6-methylguanine–DNA methyltransferase (1998)
    Copenhagen : International Union of Crystallography (IUCr)
    Acta crystallographica 54 (1998), S. 1395-1396 
    Details
    ISSN: 1399-0047
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Notes: Crystals of archaeal O6-methylguanine–DNA methyltransferase (MGMT) from hyperthermophilic archaeon Pyrococcus kodakaraensis strain KOD1 have been grown at room temperature using polyethylene glycol as a precipitant. The diffraction pattern of the crystal extends to 2.0 Å resolution at room temperature upon exposure to Cu K\alpha radiation. The crystal belongs to the space group P212121 with unit-cell dimensions of a = 52.8, b = 86.6 and c = 39.9 Å. The presence of one molecule per asymmetric unit gives a crystal volume per protein mass (Vm) of 2.3 Å3 Da−1 and a solvent content of 48% by volume. A full set of X-ray diffraction data was collected to 2.0 Å Bragg spacings from the native crystal.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1107/S0907444998001346
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  • 2
    Electronic Resource
    Electronic Resource
    Crystallographic study of intein homing endonuclease II encoded in the archaeal DNA polymerase gene (2000)
    Copenhagen : International Union of Crystallography (IUCr)
    Acta crystallographica 56 (2000), S. 1185-1186 
    Details
    ISSN: 1399-0047
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Notes: Intein homing endonucleases are proteins spliced out from a precursor protein and site-specific enzymes that make double-strand breaks in inteinless alleles. Crystals of intein homing endonuclease II from the hyperthermophilic archaeon Pyrococcus kodakaraensis strain KOD1 (PI-PkoII) have been grown at room temperature using ammonium sulfate as a precipitant. The diffraction pattern of the crystal extends to 3.0 Å resolution at room temperature upon exposure to synchrotron X-rays at KEK-PF, Japan. The crystals have symmetry consistent with space group C2221, with unit-cell parameters a = 107.6, b = 150.5, c = 146.8 Å. A full set of X-ray diffraction data were collected to 3.0 Å Bragg spacing from a native crystal with an overall Rmerge of 4.8% and a completeness of 96.6%.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1107/S0907444900008672
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Promoted proliferation of an SOD-deficient mutant of Escherichia coli under oxidative stress induced by photoexcited TiO2 (2004)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 236 (2004), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: An SOD null mutant of Escherichia coli (IM303) and its wild-type strain (MM294) were cultivated with or without sublethal oxidative stress generated from photoexcited TiO2. Concerning maximum specific growth rate of the cells, μm, measured under various conditions, the μm value of IM303 cells increased notably in the presence of TiO2 illuminated with light (I=12.5 W m−2), being about two times higher than that of the cells grown in the absence of TiO2 and light. The μm value of IM303 cells under the oxidative condition restored to a level comparable to that of wild-type MM294 cells, which coincided with the finding that the content of reactive oxygen species lowered in IM303 cells under the oxidative stress. Colony isolation was conducted to obtain the cells prevailing in the early culture phase of IM303 cells in the presence of TiO2 and light. It was found that the isolates exhibited the outgrowing properties with the increased μm values under both the conditions with and without TiO2 and light. It was also indicated that in the culture of typically selected isolate, the cells started to grow with a relatively short lag in a threonine-minus medium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.2004.tb09635.x
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    Paper (German National Licenses)
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