ISSN:
1399-0047
Source:
Crystallography Journals Online : IUCR Backfile Archive 1948-2001
Topics:
Chemistry and Pharmacology
,
Geosciences
,
Physics
Notes:
Crystals of archaeal O6-methylguanine–DNA methyltransferase (MGMT) from hyperthermophilic archaeon Pyrococcus kodakaraensis strain KOD1 have been grown at room temperature using polyethylene glycol as a precipitant. The diffraction pattern of the crystal extends to 2.0 Å resolution at room temperature upon exposure to Cu K\alpha radiation. The crystal belongs to the space group P212121 with unit-cell dimensions of a = 52.8, b = 86.6 and c = 39.9 Å. The presence of one molecule per asymmetric unit gives a crystal volume per protein mass (Vm) of 2.3 Å3 Da−1 and a solvent content of 48% by volume. A full set of X-ray diffraction data was collected to 2.0 Å Bragg spacings from the native crystal.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1107/S0907444998001346