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  • 2000-2004  (10)
  • 1
    Electronic Resource
    Electronic Resource
    1/f noise of Bi2Sr2CaCu2Oy intrinsic Josephson junctions (2001)
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 90 (2001), S. 2911-2914 
    Details
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: We have measured the low-frequency voltage noise properties across mesa stacks of intrinsic Josephson junctions in Bi2Sr2CaCu2Oy (BSCCO) single crystals as a function of bias voltage. The BSCCO mesas with area of 160×40 μm2 were fabricated on the surface of cleaved BSCCO single crystals using standard photolithography and Ar ion-milling techniques. The measured noise voltage spectrum density SV(f ) had a 1/f dependence on frequency. The magnitude of the 1/f noise spectra was found to increase monotonically with the branch number in the I–V characteristic at fixed bias current, and no anomalously enhanced 1/f noise was observed. © 2001 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.1381048
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  • 2
    Electronic Resource
    Electronic Resource
    Two stage growth of microdiamond in UHP dolomite marble from Kokchetav Massif, Kazakhstan (2003)
    Oxford, UK : Blackwell Science Inc
    Journal of metamorphic geology 21 (2003), S. 0 
    Details
    ISSN: 1525-1314
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geosciences
    Notes: The abundance and morphology of microdiamond in dolomite marble from Kumdy-kol in the Kokchetav Massif, are unusual; a previous study estimated the maximum content of diamonds in dolomite marble to be about 2700 carat ton−1. Microdiamond is included primarily in garnet, and occasionally in diopside and phlogopite pseudomorphs after garnet. They are classified into three types on the basis of their morphology: (1) S-type: star-shaped diamond consisting of translucent cores and transparent subhedral to euhedral very fine-grained outer parts; (2) R-type: translucent crystals with rugged surfaces; and (3) T-type: transparent, very fine-grained crystals. The S-type is the most abundant.Micro-Laue diffraction using a 1.6-µm X-ray beam-size demonstrated that the cores of the star-shaped microdiamond represent single crystals. In contrast, the most fine-grained outer parts usually have different orientations compared to the core. Laser–Raman studies indicate that the FWHM (Full Width at Half Maximum) of the Raman band of the core of the S-type diamond is slightly larger than that for the outer parts. Differences in morphology, crystal orientations, and in the FWHM of the Raman band between the core and the fine-grained outer-parts of S-type microdiamond suggest that the star-shaped microdiamond was formed discontinuously in two distinct stages.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1525-1314.2003.00459.x
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  • 3
    Electronic Resource
    Electronic Resource
    Coexistence of micrometastatic melanoma cells and sarcoid granulomas in all regional lymph nodes in a patient with acral melanoma (2003)
    Oxford, UK : Blackwell Science Ltd
    Clinical and experimental dermatology 28 (2003), S. 0 
    Details
    ISSN: 1365-2230
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Summary A 68-year-old woman who had been diagnosed with sarcoidosis presented with acral lentiginous melanoma (Breslow's tumour thickness, 2.6 mm; Clark's level IV) on her right heel. She underwent surgery for excision of the primary tumour and sentinel lymph node biopsy. The two sentinel lymph nodes revealed numerous sarcoidal granulomas and small nests of metastatic melanoma cells in the subcapuslar lesions. She subsequently underwent ilio-inguinal lymph node dissection. Surprisingly, all of the nine dissected nodes were mostly replaced by sarcoidal granulomas and contained melanoma micrometastases.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2230.2003.01279.x
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  • 4
    Electronic Resource
    Electronic Resource
    Single nucleotide polymorphism genotyping of the barley waxy gene by polymerase chain reaction with confronting two-pair primers (2004)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 123 (2004), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: A high-throughput single nucleotide polymorphism (SNP) genotyping procedure was developed to select amylose-free barley mutants whose waxy genes had a C- to T-base substitution in exon 5, which converted Gln-89 of the wild-type gene into a termination codon. An F2 population carrying an amylose-free waxy gene was checked for segregation. Polymerase chain reaction with confronting two-pair primers (PCR-CTPP) produced allele-specific PCR products that have different sizes and are inherited in a co-dominant manner. Two alleles of the barley waxy gene with SNP were correctly identified in parental strains using the PCR-CTPP procedure. Segregation of the SNP as detected by PCR-CTPP in an F2 population fitted the expected 1:2:1 ratio. The PCR-CTPP procedure can provide a time saving and cost-effective alternative to derived cleaved amplified polymorphic sequence in marker-assisted selection.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0523.2004.00970.x
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  • 5
    Electronic Resource
    Electronic Resource
    Inheritance and stability of the virus-resistant gene in the progeny of transgenic sweet potato (2002)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 121 (2002), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Viral diseases of sweet potato are very prevalent and often seriously damaging to the plants. In particular, the severe strain of the sweet potato feathery mottle virus (SPFMV-S) causes ‘obizyo-sohi’ disease in Japan. In order to confer viral resistance against SPFMV using current biotechnology, a transgenic sweet potato has been produced, introducing hygromycin-resistant (hpt) and SPFMV-S coat protein (CP) genes, which have shown a significant resistance to SPFMV-S. In the breeding programme, it is important to confirm that the viral resistance conferred in T0 plants can be inherited by their progeny. In the present study, progeny were obtained from crosses between the transgenic T0 and a non-transgenic variety of sweet potato. The results showed that the CP gene was inherited by the next generation and that the stability of viral resistance was also confirmed. Thus, this production system for the virus-resistant transgenic sweet potato is useful in practical breeding.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1439-0523.2002.00709.x
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  • 6
    Electronic Resource
    Electronic Resource
    The lymphoproliferative response to enzymatically digested gelatin in subjects with gelatin hypersensitivity (2000)
    Oxford BSL : Blackwell Science Ltd
    Clinical & experimental allergy 30 (2000), S. 0 
    Details
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: This study was designed to evaluate the immunogenic characteristics of enzymatically digested gelatin, ‘FreAlagin’, employing the lymphoproliferative response in subjects with gelatin hypersensitivity. Our purpose was to assess the response of primed lymphocytes to the newly developed FreAlagin and compare it to the response to conventional gelatin.〈section xml:id="abs1-2"〉〈title type="main"〉MethodsA gelatin-specific lymphocyte proliferation test (LPT) was performed in 110 children with adverse reactions to gelatin-containing vaccines, who showed positive gelatin-specific cell-mediated immunity and were thus diagnosed as having gelatin hypersensitivity. Gelatin-specific IgE was measured in all subjects. The antigenic activity of FreAlagin to lymphocytes was compared with that of conventional bovine gelatin. Positive and negative control specimens were obtained from the patients with anaphylaxis and from subjects inoculated with gelatin-free vaccine who showed no adverse reactions in order to establish the fluorometric ELISA system to determine IgE antibody to gelatin and LPT.〈section xml:id="abs1-3"〉〈title type="main"〉ResultsThe lymphocyte activity against FreAlagin was much less than that to Wako gelatin and more than half of the subjects who reacted positively to Wako gelatin had a negative LPT to FreAlagin. Although 47% of the subjects had positive LPTs to FreAlagin, all but two still had lower SIs to FreAlagin compared with Wako gelatin.〈section xml:id="abs1-4"〉〈title type="main"〉ConclusionWe conclude that the antigenic activity of FreAlagin as measured by the cell-mediated immune response is significantly less than that of conventional bovine gelatin. However, it is still necessary to perform clinical trials to show a reduced or absent clinical reactivity to FreAlagin in sensitized patients to conventional gelatin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2222.2000.00940.x
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  • 7
    Electronic Resource
    Electronic Resource
    Gelatin-specific cellular immune responses persist for more than 3 years after priming with gelatin containing DTaP vaccine (2002)
    Oxford, UK : Blackwell Science, Ltd
    Clinical & experimental allergy 32 (2002), S. 0 
    Details
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background Gelatin-specific cell-mediated immunity develops in subjects inoculated with gelatin containing DTaP vaccine. However, it is not yet known whether such established sensitization to gelatin disappears or persists with time.Objective The aim of this study was to follow the patients with gelatin sensitization elicited by DTaP vaccination for their lymphocyte responsiveness and IgE, IgG antibody specific to gelatin over several years and to compare the activities with those at the time of enrolment into the study.Methods We studied 28 subjects who developed positive lymphocyte proliferation test (LPT) after receiving gelatin containing DTaP vaccine and eight subjects who had a negative LPT after inoculation of non-gelatin DTaP. Determination of IgE, IgG antibodies and specific lymphoproliferative response directed against gelatin were performed at enrolment and on follow up.Results None of the subjects had antibody to gelatin at enrolment and none developed gelatin IgE or IgG during follow-up. There was no significant difference in the SIs of the subjects receiving gelatin DTaP (P = 0.150, 95% CI, −0.198–0.032), whereas lymphocyte activity to gelatin increased between enrolment and follow-up in the subjects with non-gelatin DTaP (P = 0.011, 95% CI, 0.063–0.338).Conclusion Gelatin-specific lymphocyte activity persists at comparable levels for more than 3 years in subjects who acquire a positive LPT response to gelatin after receiving primary DTaP vaccine containing gelatin. Furthermore, five out of eight subjects initially with negative LPT to gelatin have been shown to acquire specific LPT with time.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2745.2002.01505.x
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  • 8
    Electronic Resource
    Electronic Resource
    Measurement of Dermatophagoides mite allergens on bedding and human skin surfaces (2003)
    Oxford, UK : Blackwell Science Ltd
    Clinical & experimental allergy 33 (2003), S. 0 
    Details
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background The level of the Dermatophagoides mite group 1 (Der 1) allergens in reservoir dust has been used as an index of exposure in most studies. However, the mite allergen level in reservoir dust cannot directly reflect the personal exposure level.Objective We sought to develop a new method for quantifying the Der 1 allergens on bedding and human skin surfaces as an index of exposure to mite allergens.Methods Samples were obtained with a small adhesive tape from the forearm skin of 30 healthy volunteers and from their regularly used mattresses. The level of Der 1 allergens collected onto the adhesive tape was measured by a newly developed sensitive fluorometric ELISA for Der p 1 and Der f 1.Results The Der 1 allergens could be detected in all the samples from bedding surfaces and in 28 of the 30 samples from skin surfaces. The Der 1 levels by adhesive tape sampling from the mattresses correlated with those by reservoir dust sampling. The sampling of the skin and bedding surface with adhesive tape correlated, but skin sampling did not correlate with reservoir sampling.Conclusion The Der 1 allergens on bedding surfaces and on human skin surfaces could be quantified with a very simple sampling technique. The system developed in this study will provide a new tool for the assessment of mite allergen exposure in daily life.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2222.2003.01820.x
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  • 9
    Electronic Resource
    Electronic Resource
    Light modulation by biodegradatic poly-L-lactic acid film (2000)
    Springer
    Journal of materials science 19 (2000), S. 1537-1539 
    Details
    ISSN: 1573-4811
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006764820368
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  • 10
    Electronic Resource
    Electronic Resource
    p24/ING1-ALT1 and p47/ING1-ALT2, distinct alternative transcripts of p33/ING1 (2000)
    Springer
    Journal of human genetics 45 (2000), S. 177-181 
    Details
    ISSN: 1435-232X
    Keywords: Key words ING1 ; p33 ; Alternative splicing ; p24/ING1-ALT1 ; p47/ING1-ALT2 ; Chromosome band 13q34
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract p33/ING1s (the growth inhibitor ING1 and candidate tumor suppressor ING1) are key players in the suppressive pathways for human tumorigenesis. We analyzed their complete transcripts, primary structures, and expression. The results led us to discover two novel and related alternatively spliced transcripts encoding p24/ING1-ALT1 and p47/ING1-ALT2. They share C-terminal residues with the candidate tumor suppressors p33/ING1. The candidate tumor suppressors p33/ING1 and p24/ING1-ALT1 were coexpressed in a variety of fetal and adult human tissues, but p47/ING1-ALT2 was minimally expressed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s100380050206
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