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  • 11
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    British journal of dermatology 130 (1994), S. 0 
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Summary It has been proposed that basic fibroblast growth factor (bFGF) is an autocrine growth factor of melanoma cells, in contrast with normal melanocytes where bFGF acts as a paracrine growth factor. As this notion is mostly based on the different requirements for bFGF in cultures of benign and malignant pigment cells in vitro, we performed an immunohistochemical study to examine bFGF expression in vivo using paraffin sections from naevus cell naevi (NCN) and malignant melanoma (MM). All the NCN (n=7) showed strong and homogeneous expression of bFGF protein, whereas the primary MMs (n=5) showed heterogeneous expression, with a population of negative cells. Metastatic MMs (n=5) also showed heterogeneous expression, and had a greater population of negative cells. These results suggest that bFGF has some, as yet unidentitied, role in the growth of benign NCN, and that overexpression of bFGF is neither a prerequisite for melanoma genesis nor for progression to metastatic MM.
    Type of Medium: Electronic Resource
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  • 12
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    British journal of dermatology 126 (1992), S. 0 
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: We report the case of a 49-year-old woman suffering from a malignant neoplasm of Langerhans cells (LC), documented by immunohistochemical and ultrastructural analysis, and review the literature to examine and characterize the clinical and laboratory features, therapy, and prognosis of malignant neoplasms of LC. Langerhans cell histiocytosis is now regarded as a disorder of immune regulation or an inflammatory process, rather than as a malignant neoplasm. Although LC share certain features in common with ordinary histiocytes or interdigitating dendritic cells, they also differ significantly from these cells in other respects. Therefore, we propose designating a malignant neoplasm of LC ‘malignant Langerhans cell tumour’ and that it should be considered as a separate entity from Langerhans cell histiocytosis or other malignant histiocytoses.
    Type of Medium: Electronic Resource
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  • 13
    ISSN: 1476-5535
    Keywords: Keywords: Sphingomonas; macromolecule transport; pit; polysaccharide lyase; ABC transporter
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A bacterium isolated from soil as an alginate lyase producer shows characteristic morphological and taxonomical properties consistent with being classified in the genus Sphingomonas. The bacterium utilizes high molecular weight (HMW)-alginate for growth by depolymerization of the polymer with intracellular alginate lyases, which are generated from a common precursor protein through autoregulated post-translational modifications. Electron microscopic observations of the cell surface and of thin sections of cells grown on HMW-alginate revealed dynamic changes in both cell surface and membrane structures. The most remarkable change is recognized in the formation of mouth-like pits which open and close depending on the presence or absence of HMW-alginate. Enzymatic and genetic analyses of HMW-alginate incorporation processes confirmed the presence of a pit-dependent and macromolecule-specific ABC transporter system in cells of Sphingomonas species A1. This is the first description of a bacterium with a pit on the cell surface and a pit-dependent endocytosic uptake system for macromolecules.
    Type of Medium: Electronic Resource
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  • 14
    ISSN: 1432-069X
    Keywords: Hyperpigmentation ; UVB ; UVA ; Allergic contact dermatitis ; Guinea pig
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In moderately colored guinea-pig skin, UVB, PUVA, and allergic contact dermatitis were shown to induce hyperpigmentation that resembled the pigmentary changes observed in mongoloid human skin. Using this model, we examined the effects of chemical agents, including tyrosinase inhibitors and sunscreen agents, on the color changes induced by UV irradiation. The daily exposure of brownish guinea-pig skin to UVB irradiation at a variety of energies for 3 successive days induced clearly visible black pigmentation on the irradiated rectangular areas of the flank within a few days of irradiation, the maximum being reached about 1 week after irradiation, i.e., similar to the changes that occur in pigmented human skin. Split epidermal sheets prepared from untreated pigmented guinea pigs exhibited 200–400 melanocytes/mm2; 1 week after UV irradiation, the applied areas show an increased number of strongly dopa-positive melanocytes with stout dendrites (800–1,000 cells/mm2). UVA irradiation following an intraperitoneal injection of 8-methoxypsoralen (8-MOP) also produced black pigmentation 1 week after irradiation, and this was paralleled by a marked increase in the number of dopa-positive melanocytes in dopa-reacted split epidermal sheets. Allergic contact dermatitis produced by the application of 1-phenylazo-2-naphtol induced hyperpigmentation after an interval of about 14 days in 10 of the 21 allergy-acquiring animals examined. This induced pigmentation was accompanied by an increase in the number of dopa-positive melanocytes as compared to the number seen in controls. In contrast, allergic contact dermatitis produced by the application of dinitrochlorobenzene failed to induce such a high ratio of postpigmentation, with only 3 of the 21 allergy-acquiring animals showing hyperpigmentation and 5 showing depigmentation; in the latter, there was a slight decrease in the number of dopa-positive melanocytes. To study the preventive effect of tyrosine inhibitors on UVB-induced pigmentation, daily topical applications of these compounds were performed after three daily UVB irradiations. Treatment with 10% hydroquinone for 10 days interrupted UVB-induced pigmentation and resulted in a marked reduction in the number of epidermal melanocytes as compared to the number found in UVB-irradiated, untreated control skin.
    Type of Medium: Electronic Resource
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  • 15
    Electronic Resource
    Electronic Resource
    Springer
    Archives of dermatological research 278 (1986), S. 329-334 
    ISSN: 1432-069X
    Keywords: Melanoma metastasis ; Interferon ; Time-dependent suppression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The effect of murine α/β interferon (IFN) on experimental metastasis was investigated using B16-F10 melanoma cells. Since the outcome of metastasis of blood-borne tumor cells is mainly determined within the first 24 h after i.v. inoculation of tumor cells, i.p. injection of IFN was focused on this critical early phase. The inhibition of pulmonary metastases by IFN was found to be maximal when given 3 h prior to tumor cell inoculation, while mice with 24-h and 12-h pretreatment and simultaneous IFN treatment also showed a reduction in metastases, but to a lesser extent. However, mice receiving IFN 2 h after tumor cell inoculation did not show any reduction. Tumor cells cultured for 24 h in IFN-containing medium showed no reduction in metastases. Administration of anti-asialo GMl prior to IFN treatment was found to eliminate the inhibitory effect of IFN 3 h pretreatment. However, natural killer (NK) cell activity in vitro measured at 3 h, 13 h and 24 h after IFN administration was enhanced to the same extent, not paralleling the inhibitory effect on pulmonary metastases. These data indicate that prepared host status against blood-borne tumor cells is established by IFN pretreatment, being maximal when injected several hours prior to tumor cell inoculation, and that this effect is substantially dependent on NK cell activity, though the implication of other factors is not excluded.
    Type of Medium: Electronic Resource
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  • 16
    Electronic Resource
    Electronic Resource
    Springer
    Surgical and radiologic anatomy 13 (1991), S. 89-104 
    ISSN: 1279-8517
    Keywords: Gallbladder ; Lymphatic system ; Carcinoma ; Macroscopic anatomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Résumé A partir de la dissection fine des lymphatiques de la vésicule effectuée sur 4 sujets adultes, trois voies de drainages ont pu être schématisées. 1) La voie cholécysto-rétro pancréatique qui peut être considérée comme la principale, comporte deux trajets, l'un descendant en spirale de la face antérieure de la voie biliaire principale vers son bord postéro-droit, et l'autre descendant directement à la face postérieure du conduit biliaire. Dans leur segment rétroportal, ces voies convergent vers un important nœud lymphatique qui apparaît comme le principal nœud terminal de cette voie. Nous l'avons dénommé le nœud principal rétroportal. 2) La voie cholécysto-cœliaque est utilisée par quelques lymphatiques de la vésicule biliaire qui se dirigent vers la gauche à travers le ligament hépato-duodénal pour rejoindre les nœuds cœliaques. 3) La voie cholécysto-mésentérique est empruntée par des lymphatiques qui descendent vers la gauche en avant de la veine porte et rejoignant les nœuds situés à l'origine de l'artère mésentérique supérieure. Ces trois voies convergent vers les nœuds lymphatiques abdomino-aortiques situés à proximité de la veine rénale gauche; les nœuds situés dans l'espace inter-aortico-cave seront particulièrement importants.
    Notes: Summary Based upon the detailed dissections of the lymphatic system of four adult cadavers, the lymphatic drainage of the gallbladder was divided into three pathways. 1) The cholecysto-retropancreatic pathway, which can be regarded as the main pathway, had two routes, one running spirally and posteriorly from the anterior surface of the common bile duct to the right, and the other running almost straight down from the posterior surface of the common bile duct. At the retroportal segment, these routes converged at a large lymph node, which appeared critical as the main terminal lymph node of this pathway. We designated this node the principal retroportal node. 2) The cholecysto-celiac pathway was the route by which some of the lymphatics from the gallbladder ran to the left through the hepatoduodenal ligament to reach the celiac nodes. 3) The cholecysto-mesenteric pathway was the route by which some of the lymphatics ran to the left in front of the portal vein and connected with the nodes at the superior mesenteric root. These three pathways converged with the abdomino-aortic lymph nodes near the left renal vein; in particular, the nodes in the interaortico-caval space were considered important.
    Type of Medium: Electronic Resource
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  • 17
    Electronic Resource
    Electronic Resource
    Springer
    Journal of materials science 25 (1990), S. 4247-4254 
    ISSN: 1573-4803
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract Silicon carbide fibres (Nicalon) were coated with pure aluminium and aluminium alloys containing silicon. The coated fibres were annealed to produce an interfacial reaction zone between the coated layer and the fibre. The effect of this reaction zone on the tensile strength of the fibre was investigated. During the early stages of growth the reaction zone of the fibre is thin, and the strength of the fibre is controlled by inherent defects so that the fibre retains its original strength. After the early stages, notches are formed in the reaction zone of the fibre on loading at a small strain and the fibre fractures when a notch extends into the fibre. In this stage the fibre strength is dependent on the thickness of the reaction zone. An alloying addition of 1 or 5 at % Si to the aluminium matrix was found to be effective in retarding the growth rate of the reaction zone.
    Type of Medium: Electronic Resource
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  • 18
    Electronic Resource
    Electronic Resource
    Springer
    Journal of materials science 25 (1990), S. 3273-3280 
    ISSN: 1573-4803
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract An investigation is carried out on the interfacial wetting behaviour and reactions between aluminium and alumina fibres (85mass% Al2O3 and 15mass% SiO2). Aluminium is coated onto alumina fibres by a vacuum evaporation technique and the surface of the fully coated fibres and the edge of the partially coated fibres are examined by scanning electron microscope after heat treatments at various temperatures. Within a temperature regime between 943 and 1273 K, occurrence of such interfacial reactions as 4Al(I) + Al2O3(s) → 3Al2O3(g) and 4Al(I) + 3SiO2(s) → 2Al2O3(s) + 3Si(s) are detected. It is found that molten aluminium can cover the alumina fibre surface but it peels off near the edge of the coating film on a partially coated fibre, showing the very weak interface cohesion. This is ascribed to the lack of a stable compound formation at the interface. Results of tensile test show that the strength of the coated fibres is degraded after heat-treating at above the melting point of aluminium. The culprits for the tensile failure of alumina fibres are evaluated by the Weibull distribution theory.
    Type of Medium: Electronic Resource
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  • 19
    Electronic Resource
    Electronic Resource
    Springer
    Langenbeck's archives of surgery 372 (1987), S. 399-399 
    ISSN: 1435-2451
    Keywords: Colectomy ; Proctomucosectomy with ileo-anal anastomosis ; Maximal anal canal pressure at rest ; Colektomie ; Proktomucosektomie mit ileo-analer Anastomose ; Maximaler Analkanaldruck in der Ruhephase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Seit 1978 wurden 54 Patienten (37 Adenomatosis coli und 17 Colitis ulcerosa) durch Colektomie, Proktomucosektomie mit ileo-analer Anastomose operiert. Dabei wurde die rectale Mucosa ausgehend von einem Punkt oberhalb des peritonealen Bodens, und hinab bis zur Linea dentata abgeschält. Die Patienten, bei denen der maximale Analkanaldruck in der Ruhephase nach der Operation grössber als 60 cm Wassersäule war, zeigten alle ausgezeichnete bis gute faecale Tätigkeit. Hinsichtlich des Taschentyps ist eine J-förmige Tasche in der rectalen Mucosastulpe vorzuziehen.
    Notes: Summary Since 1978, 54 patients (37 adenomatosis coli and 17 colitis ulcerosa) have undergone colectomy, proctomucosectomy with ileo-anal anastomosis. The rectal mucosa was stripped out beginning at the level above the peritoneal floor and down to just above the dentate line. All the patients, in which the maximal anal canal pressure at rest was more than 60 cm water column after the operation, showed excellent or good fecal function. As to the pouch type constructed, J-formed pouch is most favourable.
    Type of Medium: Electronic Resource
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  • 20
    Electronic Resource
    Electronic Resource
    Springer
    Langenbeck's archives of surgery 372 (1987), S. 915-915 
    ISSN: 1435-2451
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
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