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  • 1995-1999  (35)
  • 1980-1984  (25)
  • 1965-1969  (21)
  • 1915-1919  (1)
  • 1910-1914  (12)
  • General Chemistry  (79)
  • Analytical Chemistry and Spectroscopy  (11)
  • DNA
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Material
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Year
Keywords
  • 1
    Electronic Resource
    Electronic Resource
    Fluorometric determination of DNA in epidermis and cultured fibroblasts, using 4′-6-diamidino-2-phenylindole (DAPI) (1984)
    Springer
    Archives of dermatological research 276 (1984), S. 52-56 
    Details
    ISSN: 1432-069X
    Keywords: Fluorometry ; DNA ; 4′-6-Diamidino-2-phenylindole ; Fibroblasts ; Epidermis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary 4′-6-diamidino-phenylindole (DAPI) which is used for the detection of mycoplasmic infections of cell cultures or cytofluorometric investigations proved to be a sensitive agent for the fluorometric assay of alkali extracted DNA in cultured cells and human epidermis. Various experiments to optimize assay conditions led to the following procedure: 1 part of freeze dried tissue (1 mg/ml) and 1 part of 0.2 mol/l NaOH (w/v) were heated at 95° for 40 min. After cooling for 1 h to room temperature, 0.1 ml alkaline extract was mixed with 2.9 ml DAPI-solution (0.2 μg/ml in McIlvaine buffer containing 1 mmol/l MgCl2). After 10 min in the dark the emitted fluorescence was estimated at 453 nm with an excitation at 360 nm. This assay procedure allowed a detection limit of 25 ng DNA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00412563
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Hormonal control of tobacco protoplast nucleic acid metabolism during in vitro culture (1981)
    Springer
    Planta 152 (1981), S. 1-7 
    Details
    ISSN: 1432-2048
    Keywords: Auxin ; Cytokinin ; DNA ; Nicotiana RNA ; Protoplasts
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Tobacco mesophyll protoplasts cultivated in vitro do not synthesize a measurable quantity of chloroplastic ribosomal RNA, but actively synthesize cytoplasmic ribosomal RNA, polyadenylated RNA, and proteins. These syntheses are essentially independent of the presence of hormones in the culture medium and are thus related to the ageing phenomenon induced by isolation from the plant and in-vitro culture. At all stages of culture and in all culture media, protoplasts incorporate low levels of thymidine into their DNA. However, the incorporation of considerable quantities of thymidine, indicative of the S phase, only takes place after 25–30 h and requires the presence of auxin and cytokinin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00384976
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Practical breast carcinoma cell kinetics: Review and update (1984)
    Springer
    Breast cancer research and treatment 4 (1984), S. 79-88 
    Details
    ISSN: 1573-7217
    Keywords: breast ; carcinoma ; DNA ; kinetics ; ploidy ; receptors
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The S-phase fraction (SPF) measured by flow cytometry of DNA and the thymidine labeling index (TLI) measured autoradiographically indicate the proportion of carcinoma cells currently synthesizing DNA and reflect the rate of proliferation. The TLI and SPF are lognormally distributed. The median TLI performed to maximize precursor uptake is near 5% (5 labeled carcinoma cells per 100), the mean near 7%, and the range from less than 1% to near 40%. Corresponding values for the SPF measured by DNA flow cytometry are slightly higher when appropriate measures are taken to reduce background debris counts and other artefacts. Residual elevation of SPF above TLI may result from S-phase arrested cells. Flow cytometric histograms show that clearly aneuploid cell lines exist in 50–80% of primary breast carcinomas. Aneuploid breast carcinomas have higher mean TLI than diploid breast carcinomas, and therefore proliferate more rapidly. They also more frequently lack estrogen receptor (ER). Carcinomas with minimal nuclear anaplasia, particularly those of tubular, mucinous, infiltrating lobular and adenocystic types have low TLI and SPF, whereas carcinomas with highly anaplastic nuclei, including medullary carcinomas, have high TLI and SPF. TLI and SPF correlate inversely with ER and PgR content, have no relationship to axillary lymph nodal status, and have a weak positive correlation with tumor size and a weak negative correlation with age. High TLI predicts a high risk of early relapse after primary therapy for both node-negative and node-positive carcinomas. Carcinomas that produce brain metastases have particularly high TLI. Current evidence suggests that high SPF and aneuploidy may prove to have prognostic significance like TLI.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01806389
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  • 4
    Electronic Resource
    Electronic Resource
    The Formal Total Synthesis of Epothilone A (1999)
    Weinheim : Wiley-Blackwell
    Liebigs Annalen 1999 (1999), S. 2817-2823 
    Details
    ISSN: 1434-193X
    Keywords: Epothilone A ; Ring-closing metathesis ; Aldol reactions ; Lactones ; Diastereoselective alkylation ; Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The formal total synthesis of epothilone A is described. The key steps in the synthesis of the northern hemisphere are a Z-selective ten-membered ring-closing metathesis reaction (RCM) and the diastereoselective alkylation at C8. Aldehyde 3 is formed by introduction of the thiazole moiety by a Wittig reaction and subsequent functional group transformation. An efficient route to keto acid 5 is described.
    Type of Medium: Electronic Resource
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    Paper (German National Licenses)
  • 5
    Electronic Resource
    Electronic Resource
    Analysis of C1-C3 alcohols in blood by high resolution headspace gas chromatography (1982)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 5 (1982), S. 377-378 
    Details
    ISSN: 0935-6304
    Keywords: Gas chromatography ; Glass capillary columns ; Headspace analysis ; Alcohols in blood ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240050709
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    Rapid and simple direct determination of porphyrins in urine by ion-pair reversed-phase high performance liquid chromatography (1980)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 3 (1980), S. 85-86 
    Details
    ISSN: 0935-6304
    Keywords: Liquid chromatography, HPLC ; Ion-pair reversed-phase ; Free porphyrin carboxylic acids in urine, complete separation ; Quantitation within 4.4-5.4% precision ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240030206
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  • 7
    Electronic Resource
    Electronic Resource
    Interface for capillary columns on an automatic headspace gas chromatographic system (1980)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 3 (1980), S. 357-358 
    Details
    ISSN: 0935-6304
    Keywords: Gas chromatography ; Capillary column, glass ; Interface, home-made ; Headspace analysis ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240030711
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  • 8
    Electronic Resource
    Electronic Resource
    Simultaneous Measurement of Hydrocarbons and Sulfur Compounds using Flame Ionization and Sulfur Chemiluminescence Detection for Sulfur Simulated Distillation (1999)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 22 (1999), S. 386-390 
    Details
    ISSN: 0935-6304
    Keywords: Sulfur chemiluminescence detection ; sulfur simulated distillation ; boiling point distribution ; simultaneous FID/SCD analysis ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A gas chromatographic system for the simultaneous acquisition of hydrocarbon and sulfur chromatograms was developed. Detection of sulfur compounds is achieved using a sulfur chemiluminescence detector (SCD) mounted in series with a flame ionization detector (FID). A constant fraction of the effluent of the FID is transferred to the SCD by means of a fixed restrictor. Unlike previous versions of this approach, the FID is not used to generate the chemiluminescent sulfur species. Rather, the FID is operated under optimum conditions for hydrocarbon analysis and a furnace is used to generate the chemiluminescent sulfur species. The system permits dual acquisition of the hydrocarbon and sulfur signals in a single analysis with a single column, since the detectors are operated in a serial fashion. The application of sulfur simulated distillation using this approach was examined, since this requires simultaneous universal and sulfur selective detection. Precision of absolute response of both the FID and SCD was typically less than 2% RSD for a standard reference material.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
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    Paper (German National Licenses)
  • 9
    Electronic Resource
    Electronic Resource
    Fused silica capillary columns-restoration of chromatographic efficiency for long chain alcohols (1982)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 5 (1982), S. 380-381 
    Details
    ISSN: 0935-6304
    Keywords: Gas chromatography ; Capillary, fused silica ; Thermal aging, and regeneration of fused silica capillaries ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240050711
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    Paper (German National Licenses)
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  • 10
    Electronic Resource
    Electronic Resource
    Clusters on Clusters: closo-Dodecaborate as a Ligand for Au55 Clusters (1999)
    Weinheim : Wiley-Blackwell
    Berichte der deutschen chemischen Gesellschaft 1999 (1999), S. 2051-2055 
    Details
    ISSN: 1434-1948
    Keywords: Gold clusters ; closo-Dodecaborate ; Conductivity ; Charging energy ; Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The exchange of PPh3 in Au55(PPh3)12Cl6 by Na2[B12H11SH] using a phase-transfer reaction from CH2Cl2 to water needs 6 weeks for reaction, but finally results in the quantitative formation of Au55[(B12H11SH)Na2]12Cl6 (2). Cluster 2, which is of considerable stability in aqueous solution, has been characterized by 1H-NMR, 11B-NMR, and IR spectroscopy as well as by transmission electron microscopy (TEM). The Na+ cations can be exchanged by (octyl)4N+ making the resulting cluster 4 soluble in polar organic solvents. The electrical properties of 2 and 4 are compared with those of Au55[Ph2PC6H4SO3N(octyl)4]12Cl6 (3), the ammonium derivative of the sodium salt. Cluster 3 shows the expected increase of activation energy in the temperature range of 130-250 K owing to the increase of cluster spacing compared with the sodium derivative. However, clusters 2 and 4, show electromigration in the electric field, caused by the huge amount of ionic charges in the ligand shell.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
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    Paper (German National Licenses)
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