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  • 1995-1999  (2)
  • 1980-1984  (5)
  • Biochemistry and Biotechnology  (5)
  • Anoxia  (1)
  • Cemented tungsten carbide  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Flow microfluorometry measurements of multicomponent cell composition during batch bacterial growth (1980)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 22 (1980), S. 457-462 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260220216
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Flow cytometric analysis of plasmid heterogeneity in Escherichia coli populations (1983)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 25 (1983), S. 2485-2490 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260251017
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Continuous cultivation of fission yeast: Analysis of single-cell protein synthesis kinetics (1981)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 23 (1981), S. 2315-2331 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A fundamental problem in microbial reactor analysis is identification of the relationship between environment and individual cell metabolic activity. Population balance equations provide a link between experimental measurements of composition frequency functions in microbial populations on the one hand and macromolecular synthesis kinetics and cell division control parameters for single cells on the other. Flow microfluorometry measurements of frequency functions for single-cell protein content in Schizosaccharomyces pombe in balanced exponential growth have been analyzed by two different methods. One approach utilizes the integrated form of the population balance equation known as the Collins-Richmond equation, and the other method involves optimization of parameters in assumed kinetic and cell division functional forms in order to best fit measured frequency functions with corresponding model solutions. Both data interpretation techniques indicate that rates of protein synthesis increase most in small protein content cells as the population specific growth rate increases, leading to parabolic single-cell protein synthesis kinetics at large specific growth rates. Utilization of frequency function data for an asynchronous population is shown in this case to be a far more sensitive method for determination of single-cell kinetics than is monitoring the metabolic dynamics of a single cell or, equivalently, synchronous culture analyses.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260231014
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Characterization of intracellular accumulation of poly-β-hydroxybutyrate (PHB) in individual cells of Alcaligenes eutrophus H16 by flow cytometry (1984)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 26 (1984), S. 982-987 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Poly-β-hydroxybutyrate (PHB) accumulates in individual cells of Alcaligenes eutrophus in the form of refractile bodies which alter the light-scattering properties of individual cells. Flow cytometry has been applied to measure the distributions of single-cell light-scattering intensity in Alc. eutrophus populations during batch cultivation of the organism. These measurements clearly identify heterogeneities in the inoculum which influence the lag interval prior to beginning of exponential growth. Light-scattering distributions show greater homogeneity and are extremely similar during balanced, exponential growth. After exhaustion of the nitrogen source and with carbon source still available, significant PHB accumulations occur and the flow cytometry measurements reveal extreme heterogeneity in single-cell light-scattering properties. These measurements clearly demonstrate the potential advantages of single-cell light-scattering measurements by flow cytometry for analysis and control of certain fermentation processes. Single-cell light-scat light-scattering measurements in conjunction with flow sorting instrumentation have been applied to demonstrate enrichment of PHB-producing cells, initially present in a number concentration of 0.01%by a factor of 300 in a single pass. Flow cytometry-cell sorting technology should find significant application in strain improvement and mutant selection.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260260824
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Immobilization of enzymes in porous supports: Effects of support-enzyme solution contacting (1984)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 26 (1984), S. 892-900 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Proteins have been immobilized in porous support particles held in a fixed-bed reactor through which protein solution is continuously circulated. Changing the recirculation flow rate alters the observed immobilization kinetics and the maximum enzyme loading which can be achieved for glucose oxidase and glucoamylase on carbodiimide-treated activated carbon and for glucoamylase immobilized on CNBr-Sepharose 4B. Direct microscopic examination of FITC-labelled protein in sectioned Sepharose particles and indirect activity-loading studies with activated carbon-enzyme conjugates all indicate that immobilized enzyme is increasingly localized near the outer surface of the support particles at larger recirculation flow rates. Restricted diffusion of enzymes may be implicated in this phenomenon. These contacting effects may be significant considerations in the scaleup of processes for protein impregnation in porous supports, since apparent activity and stability of the final preparation depend on internal protein distribution.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260260812
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    High-temperature wear of cemented tungsten carbide tools while machining particleboard and fiberboard (1999)
    Springer
    Journal of wood science 45 (1999), S. 445-455 
    Details
    ISSN: 1611-4663
    Keywords: Cemented tungsten carbide ; Machining ; Wear mechanism ; Particleboard ; Fiberboard
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract Published research on the wear processes of cemented tungsten carbide tools used for machining reconstituted wood products was reviewed, and the current state of knowledge in this area was evaluated. Underlying assumptions and conclusions regarding high-temperature oxidation/corrosion wear during machining were examined in view of known reaction kinetics of cemented tungsten carbide alloys in oxidative and corrosive environments at temperatures that may occur at the cutting edge. This examination indicated that some wear mechanisms other than high-temperature oxidation/corrosion are likely to be rate-controlling when machining reconstituted wood products such as particleboard and fiberboard.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00538952
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  • 7
    Electronic Resource
    Electronic Resource
    Short-term anoxia does not impair protein turnover in isolated perfused turtle heart (1995)
    Springer
    Journal of comparative physiology 164 (1995), S. 622-628 
    Details
    ISSN: 1432-136X
    Keywords: Protein turnover ; Normoxia ; Anoxia ; Perfused heart ; Turtle, Trachemys sp
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Protein turnover was studied under conditions of normoxia and anoxia in isolated perfused turtle (Trachemys (=Pseudemys) scripta elegans) hearts at 15 °C. Protein synthesis was measured by 3H-labelled phenylalanine incorporation into the protein pool. Protein degradation was studied using the protein synthesis inhibitor cycloheximide and measuring phenylalanine release from the heart. Heart rate, cardiac output and ventricle pressure development were unaffected by 2 h of anoxia. Protein synthesis rate was tenfold lower in turtle heart than in rat heart at their respective body temperatures. However, 1 h of anoxia did not affect the rate of protein synthesis in turle heat. RNA content of both ventricle and atria decreased after anoxic perfusion. Protein synthesis rates in atria were higher than those of ventricle under conditions of either anoxia or normoxia. Protein degradation rate did not change in cycloheximide-treated turtle hearts after 2 h of anoxia. These findings indicate that lack of oxygen per se does not affect protein turnover in the isolated perfused turtle heart. This feature presumably allows protein turnover to proceed in vivo during routine dives at elevated temperatures. Also, the turtle heart is a good model system to study the effects of anoxia on protein turnover without the potentially confounding factor of contractile failure. These hearts are very resistant to anoxia and well able to maintain contractility for extended periods of time.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00389803
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    Paper (German National Licenses)
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