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  • 1
    Electronic Resource
    Electronic Resource
    Functional serotonin 5-HT1D receptors and 5-HT1Dβ receptor mRNA expression in human umbilical vein endothelial cells (1995)
    Springer
    Naunyn-Schmiedeberg's archives of pharmacology 352 (1995), S. 580-582 
    Details
    ISSN: 1432-1912
    Keywords: 5-Hydroxytryptamine (5-HT, serotonin) ; 5-HT1D receptors ; 5-HT1Dβ receptors ; Endothelial cells ; Cyclic AMP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Pharmacological evidence has suggested the presence of 5-hydroxytryptamine (5-HT, serotonin), 5-HT1D receptors on endothelial cells but these receptors have never been identified unamiguously on this type of cells. We now report that human umbilical vein endothelial cells (HUVEC) express 5-HT1D receptors coupled to inhibitiion of cyclic AMP formatin. 5-HT and the 5-HT1D receptor agaonists 5-carboxamidotryptamine (5-CT) and sumatriptan were approximately equipotent at inhibiting forskolin-stimulated cyclic AMP accumulation in HUVEC (mean pEC50 7.6–8.2, maximal effect 30% inhibition). The 5-HT1A receptor agonist, 8-OH-DPAT was clearly less potent (pEC50 6.2) and less efficacious. The selective 5-HT1D receptor antagonist, GR127935 (1 nM) markedly inhibited the effct pf 5-HT (apparent pKB 10.8). Reverse transcription-polymerase chain reaction analysis showed the mRNA for 5-HT1Dβ receptors to be expressed in HUVEC. These results demonstrate the presence of functional 5-HT1D receptors and the expression of 5-HT1Dβreceptor mRNA in HUVEC. They support he involvement of 5-HT1Dβ receptors in endothelial-mediated responses to 5-HT.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00169394
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Functional serotonin 5-HT1D receptors and 5-HT1Db receptor mRNA expression in human umbilical vein endothelial cells (1995)
    Springer
    Naunyn-Schmiedeberg's archives of pharmacology 352 (1995), S. 580-582 
    Details
    ISSN: 1432-1912
    Keywords: Key words 5-Hydroxytryptamine (5-HT ; serotonin) ; 5-HT1D receptors ; 5-HT1Dβ receptors ; Endothelial cells ; Cyclic AMP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Pharmacological evidence has suggested the presence of 5-hydroxytryptamine (5-HT, serotonin), 5-HT1D receptors on endothelial cells but these receptors have never been identified unambiguously on this type of cells. We now report that human umbilical vein endothelial cells (HUVEC) express 5-HT1D receptors coupled to inhibition of cyclic AMP formation. 5-HT and the 5-HT1D receptor agonists 5-carboxamidotryptamine (5-CT) and sumatriptan were approximately equipotent at inhibiting forskolin-stimulated cyclic AMP accumulation in HUVEC (mean pEC50 7.6–8.2, maximal effect 30% inhibition). The 5-HT1A receptor agonist, 8-OH-DPAT was clearly less potent (pEC50 6.2) and less efficacious. The selective 5-HT1D receptor antagonist, GR127935 (1 nM) markedly inhibited the effect of 5-HT (apparent pKB 10.8). Reverse transcription-polymerase chain reaction analysis showed the mRNA for 5-HT1Dβ receptors to be expressed in HUVEC. These results demonstrate the presence of functional 5-HT1D receptors and the expression of 5-HT1Dβ receptor mRNA in HUVEC. They support the involvement of 5-HT1Dβ receptors in endothelial-mediated responses to 5-HT.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00169394
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Gas chromatographic-mass spectrometric determination of natural profiles of androgens, progestogens, and glucocorticoids in muscle tissue of male cattle (1999)
    Springer
    European food research and technology 209 (1999), S. 393-399 
    Details
    ISSN: 1438-2385
    Keywords: Key words Hormone pattern ; Gas chromatography-mass spectrometry ; Beef ; Bulls ; Steers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract  A rapid and economical method was developed for the simultaneous determination of androgens, progestogens, and glucocorticoids and their precursors and metabolites in meat. Steroids were isolated with liquid extraction followed by C18-solid-phase extraction (C18-SPE). Corticoids were separated from the less polar steroids by Si-SPE. The non-polar steroids required additional purification by filtration through an NH2 cartridge. The fractions were combined and derivatized with a universally applicable trimethylsilylation of both hydroxyl and keto groups. Determination was performed with gas chromatography-mass selective detection (determination limits: 0.02–0.1 μg/kg). Hormone profiles of 15 muscle tissue samples of both bulls and steers were analysed. Beef from bulls contained higher concentrations of testosterone and epitestosterone (P〈0.001), 11-deoxycorticosterone (P〈0.01), androstenedione (P〈0.05) and cortisone (P〈0.05), and significantly lower proportions of cortisol (P〈0.001) than beef from steers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002170050515
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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