Library

feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • 1995-1999  (3)
  • 42.60  (1)
  • Cryptic gene  (1)
  • D14S299  (1)
Material
Years
  • 1995-1999  (3)
Year
  • 1
    ISSN: 1437-1596
    Keywords: Key words Short tandem repeat ; D14S299 ; Japanese ; Population database ; Homoplasy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Law
    Notes: Abstract In order to increase the discriminating power of DNA analysis in personal identification, we evaluated the forensic utility of the microsatellite locus D14S299 (wg1c5) in the Japanese population and also in the Chinese and Caucasian populations. Twelve different alleles were identified in length by gel electrophoresis with silver staining. The major alleles in Japanese were sequenced and designated as the numbers of the variable repeats (GGAT or GGAA). There were five variable regions and extensive homoplasy was found. However, the allele fragment lengths were in 4 bp increments and no “interalleles” were found. The estimated heterozygosity and the polymorphism information content (PIC) were 0.726 and 0.689, respectively in Japanese. Those in Chinese (0.743 and 0.704) were similar to those in Japanese, while those in Caucasians (0.812 and 0.781) were much higher. After adjacent alleles were combined to yield at least five entries, statistical analysis was performed. The power of discrimination (PD) was 0.887 in Japanese, 0.895 in Chinese and 0.935 in Caucasians and no significant deviations from the Hardy-Weinberg equilibrium were found in the three populations. We retyped all apparently homozygous samples using an alternative pair of flanking primers and found them to be true homozygotes. D14S299 appears to be a useful STR locus for forensic practice.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 2
    ISSN: 1432-0649
    Keywords: 42.55 ; 42.60
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract We report a comparative study of a pulsed as well as continuous-wave (cw) injection seeding of a Ti:Sapphire laser pumped by aQ-switched frequency-doubled Nd3+:YAG laser for achieving narrow spectral bandwidth. The results have indicated that the Ti:Sapphire laser using either a pulsed or a cw injection seeding could achieve efficient energy extraction in a narrow spectral bandwidth. In the case of pulsed injection seeding, the injection energy required for the complete injection seeding critically depended upon the timing of the Ti:Sapphire laser with respect to the delayed onset of the slave laser. On the other hand, in the case of cw injection seeding, the spectral bandwidth of the Ti:Sapphire laser was efficiently narrowed down to approximately 0.01 cm−1 with an injection power of less than 1 mW. In both types of injection seeding, characteristics observed experimentally were compared with those obtained by a numerical simulation code based on the one-dimensional rate-equation model.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 3
    ISSN: 1617-4623
    Keywords: Streptomyces setonii ISP5395 ; Cryptic gene ; Carotenogenesis ; Sigma factor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In many species of actinomycetes, carotenogenesis can be photoinduced. The capacity to respond to photoinduction is, however unstable and, in various strains of Streptomyces, is lost at a relatively high frequency. In Streptomyces setonii ISP5395, which normally produces no carotenoids, carotenoid-producing mutants can be obtained following protoplast regeneration. We report here the characterization of a gene, crtS, which was isolated from one such mutant and can confer on wild-type S. setonii ISP5395 cells the capacity to synthesize carotenoids. Sequence analysis of crtS reveals an open reading frame, which shows homology to genes that encode alternative sigma factors in Bacillus subtilis. We propose that crtS encodes a sigma factor which is necessary for the expression of a cryptic gene(s) for carotenoid biosynthesis in S. setonii ISP5395.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...