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  • 1995-1999  (3)
  • Engineering General  (1)
  • Key words Nitrification  (1)
  • Laser scanning confocal microscopy  (1)
  • 57Q45
  • 73.40Q
  • Biochemistry and Biotechnology
  • Chemistry
Materialart
Erscheinungszeitraum
Jahr
Schlagwörter
  • 1
    ISSN: 1432-072X
    Schlagwort(e): Key words Nitrification ; Hydroxylamine-cytochrome c oxidoreductase ; Ammonia monooxygenase ; Denitrification ; Pseudomonas sp.
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Hydroxylamine oxidation was measured in four recently isolated heterotrophic nitrate-reducing bacteria belonging to the genera Pseudomonas, Moraxella, Arthrobacter and Aeromonas. A hydroxylamine-cytochrome c oxidoreductase activity was detected in periplasmic fractions of the Pseudomonas and Aeromonas spp. and in total soluble fractions of the Arthrobacter sp. A monomeric 19-kDa non-haem iron hydroxylamine-cytochrome c oxidoreductase was purified from the Pseudomonas species and shown to be similar to hydroxylamine-cytochrome c oxidoreductase of Paracoccus denitrificans.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Springer
    Protoplasma 191 (1996), S. 215-219 
    ISSN: 1615-6102
    Schlagwort(e): Laser scanning confocal microscopy ; Multiple cell layers ; Plant microtubules ; Plant microfilaments ; Roots ; Tissue clearing
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary A protocol was developed to observe plant microtubules and actin microfilaments in large tissue samples without physical sectioning. Rye (Secale cereale L. cv. Rymin) root tip pieces from two-day-old seedlings were fixed and processed for immunolabeling. Incubation times of 24–48 h were required to insure adequate penetration of fixatives, antibodies, and washing buffers. Clearing of the tissue with methyl salicylate reduced background auto-fluorescence that would otherwise interfere with the resolution of cytoskeletal structures. Microtubules or microfilaments in 5–7 cell layers were visualized using the optical-sectioning capability of laser scanning confocal microscopy (LSCM) and projected as three-dimensional images. The three-dimensional character of the cytoskeletal elements is retained when viewing stained cells of intact tissue. The net-like character of a microfilament array radiating out from a single point into the cytoplasm is maintained when the cells are stained in intact root tip pieces and imaging is accomplished in situ.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    International Journal for Numerical and Analytical Methods in Geomechanics 20 (1996), S. 331-349 
    ISSN: 0363-9061
    Schlagwort(e): elasticity ; buried pipes ; surface heading ; soil-structure interaction ; Engineering ; Engineering General
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Architektur, Bauingenieurwesen, Vermessung , Geologie und Paläontologie
    Notizen: A method is presented which may be used to compute the displacements, strains and moments (both in-plane and transverse) in buried structures such as pipelines and culverts subjected to longitudinal bending. This type of bending can occur if a surface loading such as a vehicular loading or an embankment loading is applied to the soil above the pipe or culvert.Fourier transforms are used to reduce the three-dimensional problem to one involving only two spatial directions, thereby reducing the data preparation and computation time. Conventional finite element analysis is used to approximate the field quantities in the transformed two-dimensional plane. Two Fourier integral element types have been developed which have many applications in geotechnical engineering.
    Zusätzliches Material: 10 Ill.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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