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  • 1995-1999  (6)
  • 82.50  (2)
  • Breast tumor metastases  (2)
  • sympathetic hyperactivity  (2)
  • cDNA cloning
  • 1
    ISSN: 1432-0649
    Keywords: 07.75 ; 33.00 ; 82.50
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract A MultiPhoton-Ionization Time-Of-Flight Mass Spectrometry (MPI/TOFMS) apparatus was developed for real-time measurement of the uranium isotopic ratio in nascent UF5 formed by the 266 nm photolysis of effusive UF6 ( 〈 300 K, ≈ 1.3 × 10−4 Pa). The UF5 was selectively and efficiently multiphoton ionized by 532 nm radiation at appreciably low fluences ( 〈 10 J/cm2). The main ions observed, U+ and U2+, were subsequently analyzed with a TOFMS with mass resolution of 1190 to separate235U n+ and238U n+ completely. The isotopic ratio measurements showed good precision resulting from the excellent agreement which was observed between the isotopic ratios in UF5 products and those in a parent UF6 sample. These results suggested that the MPI/TOFMS method can be applied to the real-time analysis of separation factors in the molecular laser isotope separation of uranium by ionization of UF5 following the infrared photodissociation of UF6.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-0649
    Keywords: 82.50 ; 33.00
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract In infrared multiphoton dissociation of supercooled UF6 irradiated with multifrequency para-H2 Raman laser beams, separation factors and contrast ratios were measured by selective multiphoton ionization of the photoproduct UF5 at 532 nm followed by time-of-flight mass spectrometric analysis. The relationship between separation factor and contrast ratio is discussed theoretically and quantitatively investigated in the experiments. From this relationship, we obtain the irradiation conditions of the laser-beam fluences for attaining a high separation factor and estimate the value of the intrinsic separation factor in multifrequency dissociation of UF6.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-0533
    Keywords: Key words Stress-response protein 90 ; Heat-shock ; protein 90 ; Brain tumors ; Meningiomas ; Breast tumor metastases
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract This retrospective study deals with the expression of stress-response (heat-shock) protein 90 (srp 90) in a series of 148 human brain tumors. Immunohistochemical procedures were employed; cells of the human breast cancer line MCF 7 exposed to hyperosmolar stress served as positive controls. Deposits of reaction products were found in the cytoplasm and they displayed a granular pattern. srp 90 was detected in 14/31 meningiomas and 5/10 breast cancer metastases to the brain. The protein was also present in 6/13 glioblastomas and 7/18 astrocytomas. In addition, a positive reaction was found in 2/10 medulloblastomas, 2/14 primitive neuroectodermal tumors, 1/11 pituitary tumor, 2/21 schwannomas and 2/11 lung tumor metastases; however, oligodendrogliomas and primary malignant lymphomas were not stained. The srp 90 was detected in Western blots of meningioma tissue homogenates. No significant immunohistochemical reaction was seen with sections of normal human cerebra, brain stem, cerebella, pituitary glands and spinal cords. These results document the expression of srp 90 by a variety of primary and metastatic intracranial tumors.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1432-0533
    Keywords: Stress-response protein 90 ; Heat-shock protein 90 ; Brain tumors ; Meningiomas ; Breast tumor metastases
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract This retrospective study deals with the expression of stress-response (heat-shock) protein 90 (srp 90) in a series of 148 human brain tumors. Immunohistochemical procedures were employed; cells of the human breast cancer line MCF 7 exposed to hyperosmolar stress served as positive controls. Deposits of reaction products were found in the cytoplasm and they displayed a granular pattern. srp 90 was detected in 14/31 meningiomas and 5/10 breast cancer metastases to the brain. The protein was also present in 6/13 glioblastomas and 7/18 astrocytomas. In addition, a positive reaction was found in 2/10 medulloblastomas, 2/14 primitive neuroectodermal tumors, 1/11 pituitary tumor, 2/21 schwannomas and 2/11 lung tumor metastases; however, oligodendrogliomas and primary malignant lymphomas were not stained. The srp 90 was detected in Western blots of meiningioma tissue homogenates. No significant immunohistochemical reaction was seen with sections of normal human cerebra, brain stem, cerebella, pituitary glands and spinal cords. These results document the expression of srp 90 by a variety of primary and metastatic intracranial tumors.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1619-1560
    Keywords: amyotrophic lateral sclerosis ; down-regulation ; adrenoceptors ; sympathetic hyperactivity ; circulatory collapse ; norepinephrine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Intravenous norepinephrine infusion test was performed in eight patients with amyotrophic lateral sclerosis (ALS) supported by respirators and nine control subjects, to examine α-adrenoceptor function of peripheral resistant blood vessels. Baseline plasma norepinephrine concentrations in ALS patients were significantly higher than those in control subjects, indicating basal sympathetic hyperactivity (normal 218.2 ± 59.7 pg/ml; ALS 450.0 ± 288.4 pg/ml). The stimulus-response curves in the patients were similar to those in control subjects, and there were no significant differences between mean gains of the stimulus—response curves in both groups (normal 18.7 ± 5.5; ALS 15.2 ± 11.2). However, three ALS patients, two of whom had circulatory fluctuation and sympathetic hyperactivity, revealed lower gain levels than the mean minus 2 SD in control subjects (4.7, 1.1 and 3.7). This indicates hyposensitivity or down-regulation of the α-adrenoceptor function of peripheral blood vessels in these ALS patients. For early detection of sympathetic hyperactivity and prediction of circulatory collapse, it would be useful to measure the plasma norepinephrine concentration and the gain of the norepinephrine infusion curve in respirator-dependent ALS patients.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1619-1560
    Keywords: amyotrophic lateral sclerosis ; hypertensive attacks ; sympathetic hyperactivity ; phentolamine ; propranolol
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Blood pressure changes in response to intravenous drug administration were examined in a respirator-dependent 49-year-old patient with sporadic amyotrophic lateral sclerosis (ALS) who developed severe hypertensive attacks. She showed severe hypertension and tachycardia during the daytime and nocturnal hypotension without compensatory tachycardia, which were consistent with the autonomic phenomenon reported in ALS patients. Infusion of phentolamine (2.5 mg) induced an abrupt 90 mmHg decrease in systolic pressure and slight increase in heart rate. Propranolol (1 mg) infusion induced decreases in both systolic pressure (36 mmHg) and heart rate (17 beats/min), although the pressure decrease was transient while the heart rate remained at the decreased level. Infusion of diazepam (10 mg) induced a 47 mmHg decrease in systolic pressure and a 23 beats/min increase in heart rate. These vasomotor responses indicate the distinct participation of abnormally augmented sympathetic tone, and especially of α-sympathetic hyperactivity rather than of β-sympathetic hyperactivity, in the hypertensive attacks occurring in this ALS patient.
    Type of Medium: Electronic Resource
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