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  • 1995-1999  (4)
  • Transcription termination  (2)
  • Activin receptor  (1)
  • CGH  (1)
Source
Material
Years
  • 1995-1999  (4)
Year
Keywords
  • 1
    Electronic Resource
    Electronic Resource
    Localization of nusA-suppressing amino acid substitutions in the conserved regions of the β′ subunit of Escherichia coli RNA polymerase (1996)
    Springer
    Molecular genetics and genomics 251 (1996), S. 699-706 
    Details
    ISSN: 1617-4623
    Keywords: Key words RNA polymerase ; β′ subunit ; NusA ; Transcription termination ; Rho factor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Escherichia coli RNA polymerase is composed of four different subunits, α (present in two copies), β, β′ and σ. Among these, the β′ polypeptide shares nine conserved regions with the largest subunits of eukaryotic RNA polymerases, but its role is poorly understood. We isolated novel mutations in a plasmid-borne copy of rpoC, which encodes β′, as dominant suppressors of two temperature-sensitive nusA alleles. All 20 suppressors of nusA11 (single missense mutation) isolated had either of two specific substitutions: Lys for Glu-402 (rpoC10) and Thr for Ala-904 (rpoC111) in the β′ subunit. In vivo and in vitro transcription assays revealed that the rpoC10 allele of β′ participates in Rho-dependent transcription termination. On the other hand, of 20 suppressors of nusA134 (deletion of C-terminal one-third) scattered at 18 distinct sites, 16 were assigned to one of six conserved regions C-I. These results suggested that the conserved domains of the β′ subunit of E. coli RNA polymerase are involved in transcript termination or interaction with termination factor(s).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02174119
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Localization ofnusA-suppressing amino acid substitutions in the conserved regions of theβ′ subunit ofEscherichia coli RNA polymerase (1996)
    Springer
    Molecular genetics and genomics 251 (1996), S. 699-706 
    Details
    ISSN: 1617-4623
    Keywords: RNA polymerase ; β′ subunit ; NusA ; Transcription termination ; Rho factor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Escherichia coli RNA polymerase is composed of four different subunits,α (present in two copies),β, β′ andσ. Among these, theβ′ polypeptide shares nine conserved regions with the largest subunits of eukaryotic RNA polymerases, but its role is poorly understood. We isolated novel mutations in a plasmidborne copy ofrpoC, which encodesβ′, as dominant suppressors of two temperature-sensitivenusA alleles. All 20 suppressors ofnusA11 (single missense mutation) isolated had either of two specific substitutions: Lys for Glu-402 (rpoC10) and Thr for Ala-904 (rpoC111) in theβ′ subunit. In vivo and in vitro transcription assays revealed that therpoC10 allele ofβ′ participates in Rho-dependent transcription termination. On the other hand, of 20 suppressors ofnusA134 (deletion of C-terminal one-third) scattered at 18 distinct sites, 16 were assigned to one of six conserved regions C-I. These results suggested that the conserved domains of theβ′ subunit ofE. coli RNA polymerase are involved in transcript termination or interaction with termination factor(s).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02174119
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Genomic organization and mapping of the human activin receptor type IIB (hActR-IIB) gene (1998)
    Springer
    Journal of human genetics 43 (1998), S. 132-134 
    Details
    ISSN: 1435-232X
    Keywords: Key words Genomic structure ; Activin receptor ; Large-scale DNA sequencing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Activins, members of a family of proteins that includes transforming growth factor-beta (TGF-beta), are gonadal polypeptide hormones that stimulate secretion of follicle-stimulating hormone (FSH). During large-scale sequencing analysis of a 1.2-Mb fragment of human genomic DNA on 3p22–p21.3, we found the gene encoding activin receptor type IIB (hActR-IIB). Comparison of its reported cDNA sequence with this genomic sequence showed that the hActR-IIB gene consists of 11 exons and spans about 30 kb of genomic DNA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s100380050054
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Chromosomal imbalances in adult T-cell leukemia revealed by comparative genomic hybridization: gains at 14q32 and 2p16-22 in cell lines (1999)
    Springer
    Journal of human genetics 44 (1999), S. 357-363 
    Details
    ISSN: 1435-232X
    Keywords: Key words ATL ; CGH ; 14q32 ; TCL1 ; 2p ; HTLF
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Comparative genomic hybridization was used to identify chromosomal imbalances in eight cell lines and 12 blood samples from patients with adult T-cell leukemia/lymphoma (ATL). The chromosomes most often over-represented in the cell lines were 2p (6 cases), 7q (4 cases), and 14q (4 cases), with minimal common regions at 2p16-22, 7q21-36, and 14q32, respectively. Distinct imbalances were detected in only 7 of the clinical samples. Chromosomes 14q32 and 2p16-22 harbor TCL1 and a transcription factor, HTLF (human T-cell leukemia virus enhancer factor), respectively. FISH analysis revealed that TCL1 did not juxtapose to TCRA, and we detected no expression of TCL1 in any of the ATL cell lines despite the 14q32 amplifications. Moreover, expression of HTLF was not elevated in the ATL cell lines bearing multiplication of 2p. These results suggest that chromosomal regions 2p16-22 and 14q32 harbor genes other than HTLF and TCL1 that are involved in cellular immortalization or in the pathogenesis of ATL.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s100380050178
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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