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  • 1
    Electronic Resource
    Electronic Resource
    Hydrogen peroxide-induced cellular injury is associated with increase in endogenous fluorescence from rat gastric mucosal epithelial cell culture: A new method for detecting oxidative cellular injury by fluorescence measurement (1998)
    Springer
    Journal of gastroenterology 33 (1998), S. 318-325 
    Details
    ISSN: 1435-5922
    Keywords: Key words: autofluorescence ; oxygen radical ; gastric mucosal cell ; RGM1 ; cellular injury
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract: To develop a new method of detecting cellular injury caused by oxygen radicals, we studied endogenous fluorescence from the cultured cells of a rat gastric mucosal epithelial cell line. Measurement with an ultra-high sensitivity camera-image processor system under an inverted epifluorescence microscope showed that the fluorescence intensity of the cells increased time- and dose-dependently after the addition of hydrogen peroxide (H2O2), an oxygen radical precursor, to the medium. This increase was inhibited by the presence of catalase. Phase-contrast and fluorescence microscopy revealed that the fluorescence was emitted from granular substances in the cytoplasm of the injured cells. The spectral pattern of excitation and emission indicated that the fluorescent substances were flavins. In cell-free experiments, glutathione reductase which has flavin adenine dinucleotide (FAD) at the active site, increased in fluorescence after incubation with H2O2 in the presence of reduced glutathione and glutathione peroxidase. These findings indicate that FAD in the cytoplasm of cells injured by H2O2 increased in endogenous fluorescence according to the extent of injury, and suggest that fluorescence measurement may be a simple method in cellular toxicology to detect oxygen radical-induced injuries.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s005350050090
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Primary tubular adenocarcinoma arising in the duodenal limb of reconstructed gastroduodenostomy for signet-ring cell carcinoma of the stomach (1995)
    Springer
    Journal of gastroenterology 30 (1995), S. 657-660 
    Details
    ISSN: 1435-5922
    Keywords: duodenal cancer ; gastric cancer ; metachronous cancer ; adenocarcinoma
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Adenocarcinoma of the small intestine is uncommon. Due to this paucity and the lack of specificity of symptoms, patients are usually seen late in the course of their illness, when curative therapy, mainly represented by extensive surgical resection, is unlikely. The authors report a case of primary well-differentiated tubular adenocarcinoma (T4N0M0) arising in the duodenal limb of a reconstructed Billroth I gastroduodenostomy, 9 years after a distal gastrectomy for signet-ring cell carcinoma of the stomach (T4N0M0). Evidence for excluding the possibility of a recurrence of the primary gastric cancer was based on the different histologic pattern, the long disease-free interval, and other features of the second neoplasm. Relatively early diagnosis of the neoplasm, followed by curative surgical therapy was made possible by the early onset of the obstructive symptoms and the favorable anatomical location of the tumor.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02367794
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Autofluorescence in indomethacin-induced gastric mucosal lesions in rats (2000)
    Springer
    Journal of gastroenterology 35 (2000), S. 510-517 
    Details
    ISSN: 1435-5922
    Keywords: Key words: autofluorescence ; gastric injury ; non-steroidal anti-inflammatory drugs ; oxygen radicals ; porphyrins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract: Autofluorescence observations enable scientists to sensitively identify various lesions. Non-steroidal anti-inflammatory drugs such as aspirin and indomethacin are well known to induce gastric mucosal injuries. Our purpose was to clarify whether the observation of mucosal autofluorescence could help us to recognize indomethacin-induced gastric lesion formation. Gastric mucosal fluorescence intensity and gastric lesion scores were time-sequentially measured after indomethacin treatment in rats. To identify the localization of autofluorescent substances, stomach cryosections were observed with an epifluorescence microscope. Fluorescent substances from damaged tissue were also analyzed by high-performance liquid chromatography. In addition, to elucidate whether oxidative stress directly generates fluorescent substances from heme, we investigated the reaction between hydrogen peroxide and hemoglobin in a cell-free system. Treatment with indomethacin induced gastric lesions by tissue peroxidation, with mucosal fluorescence intensity increasing time-dependently. The fluorescence products were mesoporphyrin and protoporphyrin, and they were localized in disrupted mucosal tissue. In the cell-free system, porphyrins were directly generated by hydrogen peroxide from hemoglobin. These findings indicate that indomethacin treatment increased the intensity of porphyrin fluorescence. Gastric mucosal lesion formation can be sensitively detected with fluorescence observations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s005350070073
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  • 4
    Electronic Resource
    Electronic Resource
    Genomic organization and mapping of the human activin receptor type IIB (hActR-IIB) gene (1998)
    Springer
    Journal of human genetics 43 (1998), S. 132-134 
    Details
    ISSN: 1435-232X
    Keywords: Key words Genomic structure ; Activin receptor ; Large-scale DNA sequencing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Activins, members of a family of proteins that includes transforming growth factor-beta (TGF-beta), are gonadal polypeptide hormones that stimulate secretion of follicle-stimulating hormone (FSH). During large-scale sequencing analysis of a 1.2-Mb fragment of human genomic DNA on 3p22–p21.3, we found the gene encoding activin receptor type IIB (hActR-IIB). Comparison of its reported cDNA sequence with this genomic sequence showed that the hActR-IIB gene consists of 11 exons and spans about 30 kb of genomic DNA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s100380050054
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    Paper (German National Licenses)
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