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  • 1
    Electronic Resource
    Electronic Resource
    Altered expression of src tyrosine kinase after axotomy (1997)
    Springer
    Acta neuropathologica 93 (1997), S. 438-442 
    Details
    ISSN: 1432-0533
    Keywords: Key words src ; Tyrosine kinase ; Axotomy ; Nerve ; regeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Although most peripheral neurons show regenerative changes after axotomy, neurons of some nuclei show degenerative changes. To study the mechanisms responsible for such differences in neuronal reactions after axotomy, we investigated the changes of pp60c-src and phosphotyrosine (pTyr) in the dorsal motor nucleus of the vagus nerve and the hypoglossal nucleus after transection of each nerve in adult rats using immunohistochemistry. Polyclonal antibody against pp60v-src stained the cytoplasm of neurons homogeneously in both the severed and non-severed sides of the vagal and hypoglossal nuclei from days 1 to 56 after axotomy. On the other hand, the monoclonal antibody against pp60v-src (mAb327, which recognizes src homology 3 region in pp60src as an epitope) showed different immunoreactivities. No pp60c-src immunoreactivity was observed in neurons in either the vagal and hypoglossal nuclei of the non-severed side between days 1 and 56, whereas in the severed side of the hypoglossal nucleus intense pp60c-src immunoreactivity was observed along the plasma membrane of neurons from days 5 to 28 after axotomy. In contrast, in the severed side of the vagal nucleus pp60c-src immunoreactivity appeared along the plasma membrane of neurons on day 5 and remained until day 7. Conformational changes accompanying the activation of pp60c-src are suggested to cause differences in immunoreactivities of these antibodies. The expression (or activation) of pp60c-src in the neurons was stronger and longer lasting in the hypoglossal nucleus, which regenerates successfully after axotomy, than in the dorsal motor nucleus of the vagus nerve, which undergoes necrobiotic reaction. These results indicate that pp60c-src plays an important role in regeneration after axotomy.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004010050637
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  • 2
    Electronic Resource
    Electronic Resource
    p53 accumulation and apoptosis in embolized meningiomas (1997)
    Springer
    Acta neuropathologica 93 (1997), S. 599-605 
    Details
    ISSN: 1432-0533
    Keywords: Key words Apoptosis ; Meningioma ; MIB-1 ; p21 ; p53
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Preoperative embolization of meningiomas is performed to decrease blood loss at surgery. While it is also expected to reduce tumor recurrence by producing necrosis at the site of dural attachment, very little has been described about what happens to the non-necrotic tumor cells. We investigated how the proliferative activities of meningiomas were modified after embolization. In nine meningiomas which were embolized preoperatively, proliferative potentials and expression of cell cycle inhibitors were assessed immunohistochemically using MIB-1, anti-p53 (DO-1 and DO-7), and anti-p21 (WAF1/CIP1) monoclonal antibodies. To determine whether a cell underwent apoptotic death besides necrosis, we applied the terminal deoxynucleotidyl transferase-mediated dUTP-digoxigenin nick end labeling method. Results were compared with control meningiomas without embolization. MIB-1-positive cells often gathered in perinecrotic areas, although the mean MIB-1 staining index of the embolized meningiomas was not significantly different from the control. p53 and its downstream effector p21 accumulated mainly in the perinecrotic areas in eight of the nine embolized meningiomas. Apoptosis was also observed in the concomitant areas. Double staining for both MIB-1 and p21 frequently showed positive cells for both antibodies. The accumulation of MIB-1-positive cells in the embolized meningiomas may not be a sign of fast growth or malignancy, but it may implicate arrest of cell cycle by the p21. This study indicates that embolized meningiomas exhibit not only necrosis but also apoptosis and cell cycle arrest. The latter effects appear to be at least partly p53 dependent.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004010050657
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  • 3
    Electronic Resource
    Electronic Resource
    Potential doubling time and tumour doubling time in meningiomas and neurinomas (1996)
    Springer
    Acta neurochirurgica 138 (1996), S. 763-770 
    Details
    ISSN: 0942-0940
    Keywords: Cell kinetics ; Ki-67 ; meningioma ; neurinoma
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Cell kinetic study plays an important role in treatment planning of brain tumour patients. MIB-1 antibody has recently become available, which detects Ki-67 antigen even in the formalin-fixed paraffin-embedded specimens. We performed MIB-1 immunostaining in 50 meningiomas and 50 neurinomas, and estimated the cell cycle time (tc) and potential doubling time (Tpot) from MIB-1 staining index (MIB-1 SI) and mitotic index (MI). MIB-1 SI logarithmically correlated with MI in both meningiomas and neurinomas. The tc and the Tpot were expressed as a function of the mitosis time (tm), while the tm is known to be around one hour and not exceeding two hours. When the tm was assumed to be one hour, the average tcs of meningiomas and neurinomas were 6.53±3.56 days and 7.67±3.27 days, respectively. The Tpots were447 × (MIB-1 SI)−1.29 × tm in meningiomas, and490 × (MIB-1 SI) −0.98 × tm in neurinomas. The tumour doubling times (Tds) were calculated from serial imaging studies in 22 neurinomas and 15 meningiomas. The Tds were formulated as794 × (MIB-1 SI) −0.83 in meningiomas and1380 × (MIB-1 SI) −0.97 in neurinomas. Most of the Tds correlated well with the Tpots in meningiomas and neurinomas, and exceeded values of the Tpot when the tm is assumed to be one hour, although a few tumours showed unexpectedly longer Tds. The Tpot and the tc estimated from MIB-1 SI and MI are clinically useful parameters for predicting the growth potential of meningiomas and neurinomas where no other simple methods are available.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01411485
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  • 4
    Electronic Resource
    Electronic Resource
    Intradiploeic inclusion cyst lined with upper respiratory epithelium (1997)
    Springer
    Acta neurochirurgica 139 (1997), S. 793-794 
    Details
    ISSN: 0942-0940
    Keywords: Inclusion cyst ; skull tumour ; children
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01420057
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  • 5
    Electronic Resource
    Electronic Resource
    Transplantation of basal forebrain cells of foetal rats into the subarachnoid space: Improvement of disturbance of passive avoidance memory due to injury of nucleus basalis magnocellularis (1995)
    Springer
    Acta neurochirurgica 133 (1995), S. 68-72 
    Details
    ISSN: 0942-0940
    Keywords: Alzheimer's disease ; nucleus basalis magnocellularis ; neural transplantation ; subarachnoid space ; passive avoidance memory
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Basal forebrain cells of foetal rats were transplanted into the subarachnoid space of adult rats harbouring a kainic acid-induced unilateral lesion in the nucleus basalis magnocellularis. Passive avoidance response tests were performed eight weeks after the transplantation, and the results were compared with those of lesioned but non-transplanted rats and of non-lesioned control rats. Although acquisition impairments did not improve, retention impairments were significantly ameliorated in the transplanted rats. Histologically, transplanted foetal neurons survived and grew very well over the cortical surface, and exhibited facilitated neuritic elongation on acetylcholinesterase staining. Choline acetyl-transferaseimmunoreactive neurons were found along the needle track as well as in the subarachnoid graft tissues. The results seem to indicate that not the re-innervation from the graft to the host cortex but the diffusional supply of neurotransmitters and/or their synthetic enzymes and neurotrophic factors were responsible for improvement of memory deficits. The subarachnoid space proved to be an adequate place for growth of transplanted neuronal and glial cells for reasons of ample supply of oxygen and nutrition and of low tissue pressure.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01404951
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